Experiment: M9 plate, Mixed culture; Micromonospora sp. SAI_152 1:1 starding OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00525 and MPMX20_00526 overlap by 4 nucleotides MPMX20_00526 and MPMX20_00527 are separated by 85 nucleotides MPMX20_00527 and MPMX20_00528 are separated by 19 nucleotides
MPMX20_00525: MPMX20_00525 - D-glucosaminate-6-phosphate ammonia lyase, at 540,828 to 541,946
_00525
MPMX20_00526: MPMX20_00526 - 2-dehydro-3-deoxy-phosphogluconate aldolase, at 541,943 to 542,683
_00526
MPMX20_00527: MPMX20_00527 - 6-phosphogluconolactonase, at 542,769 to 543,902
_00527
MPMX20_00528: MPMX20_00528 - putative licABCH operon regulator, at 543,922 to 545,832
_00528
Position (kb) 542
543
544 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 541.792 kb on - strand, within MPMX20_00525 at 541.809 kb on - strand, within MPMX20_00525 at 541.809 kb on - strand, within MPMX20_00525 at 541.863 kb on + strand at 541.871 kb on - strand at 541.871 kb on - strand at 541.873 kb on + strand at 541.881 kb on - strand at 541.920 kb on + strand at 541.920 kb on + strand at 541.920 kb on + strand at 541.921 kb on + strand at 541.929 kb on - strand at 541.929 kb on - strand at 542.048 kb on - strand, within MPMX20_00526 at 542.117 kb on - strand, within MPMX20_00526 at 542.117 kb on - strand, within MPMX20_00526 at 542.120 kb on - strand, within MPMX20_00526 at 542.135 kb on - strand, within MPMX20_00526 at 542.169 kb on + strand, within MPMX20_00526 at 542.187 kb on - strand, within MPMX20_00526 at 542.224 kb on + strand, within MPMX20_00526 at 542.354 kb on - strand, within MPMX20_00526 at 542.361 kb on + strand, within MPMX20_00526 at 542.361 kb on + strand, within MPMX20_00526 at 542.369 kb on - strand, within MPMX20_00526 at 542.369 kb on - strand, within MPMX20_00526 at 542.369 kb on - strand, within MPMX20_00526 at 542.438 kb on + strand, within MPMX20_00526 at 542.446 kb on - strand, within MPMX20_00526 at 542.512 kb on - strand, within MPMX20_00526 at 542.532 kb on + strand, within MPMX20_00526 at 542.556 kb on + strand, within MPMX20_00526 at 542.556 kb on + strand, within MPMX20_00526 at 542.564 kb on - strand, within MPMX20_00526 at 542.585 kb on + strand, within MPMX20_00526 at 542.653 kb on + strand at 542.659 kb on - strand at 542.691 kb on + strand at 542.697 kb on + strand at 542.705 kb on - strand at 542.705 kb on - strand at 542.717 kb on - strand at 542.717 kb on - strand at 542.717 kb on - strand at 542.723 kb on + strand at 542.723 kb on + strand at 542.723 kb on + strand at 542.723 kb on + strand at 542.723 kb on + strand at 542.723 kb on + strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.731 kb on - strand at 542.776 kb on + strand at 542.776 kb on + strand at 542.784 kb on - strand at 542.835 kb on - strand at 542.839 kb on - strand at 542.863 kb on - strand at 542.877 kb on - strand at 542.877 kb on - strand at 542.882 kb on - strand at 542.884 kb on + strand, within MPMX20_00527 at 542.930 kb on + strand, within MPMX20_00527 at 542.930 kb on + strand, within MPMX20_00527 at 542.938 kb on - strand, within MPMX20_00527 at 542.938 kb on - strand, within MPMX20_00527 at 542.942 kb on + strand, within MPMX20_00527 at 542.950 kb on - strand, within MPMX20_00527 at 542.950 kb on - strand, within MPMX20_00527 at 542.957 kb on + strand, within MPMX20_00527 at 543.001 kb on + strand, within MPMX20_00527 at 543.001 kb on + strand, within MPMX20_00527 at 543.009 kb on - strand, within MPMX20_00527 at 543.027 kb on - strand, within MPMX20_00527 at 543.029 kb on + strand, within MPMX20_00527 at 543.043 kb on + strand, within MPMX20_00527 at 543.043 kb on + strand, within MPMX20_00527 at 543.053 kb on + strand, within MPMX20_00527 at 543.066 kb on - strand, within MPMX20_00527 at 543.082 kb on + strand, within MPMX20_00527 at 543.090 kb on - strand, within MPMX20_00527 at 543.097 kb on + strand, within MPMX20_00527 at 543.117 kb on + strand, within MPMX20_00527 at 543.118 kb on + strand, within MPMX20_00527 at 543.125 kb on - strand, within MPMX20_00527 at 543.125 kb on - strand, within MPMX20_00527 at 543.195 kb on - strand, within MPMX20_00527 at 543.218 kb on - strand, within MPMX20_00527 at 543.307 kb on - strand, within MPMX20_00527 at 543.353 kb on + strand, within MPMX20_00527 at 543.366 kb on - strand, within MPMX20_00527 at 543.394 kb on + strand, within MPMX20_00527 at 543.521 kb on + strand, within MPMX20_00527 at 543.534 kb on + strand, within MPMX20_00527 at 543.542 kb on - strand, within MPMX20_00527 at 543.576 kb on - strand, within MPMX20_00527 at 543.583 kb on - strand, within MPMX20_00527 at 543.716 kb on - strand, within MPMX20_00527 at 543.733 kb on + strand, within MPMX20_00527 at 543.741 kb on - strand, within MPMX20_00527 at 543.756 kb on - strand, within MPMX20_00527 at 543.756 kb on - strand, within MPMX20_00527 at 543.775 kb on + strand, within MPMX20_00527 at 543.789 kb on + strand at 543.833 kb on - strand at 543.844 kb on + strand at 543.847 kb on - strand at 543.847 kb on - strand at 543.852 kb on - strand at 543.854 kb on + strand at 543.864 kb on - strand at 543.896 kb on + strand at 543.897 kb on + strand at 543.912 kb on + strand at 543.912 kb on + strand at 543.912 kb on + strand at 543.912 kb on + strand at 543.920 kb on - strand at 543.920 kb on - strand at 543.920 kb on - strand at 543.920 kb on - strand at 543.920 kb on - strand at 544.014 kb on - strand at 544.080 kb on + strand at 544.141 kb on - strand, within MPMX20_00528 at 544.151 kb on + strand, within MPMX20_00528 at 544.151 kb on + strand, within MPMX20_00528 at 544.151 kb on + strand, within MPMX20_00528 at 544.151 kb on - strand, within MPMX20_00528 at 544.160 kb on + strand, within MPMX20_00528 at 544.177 kb on + strand, within MPMX20_00528 at 544.259 kb on - strand, within MPMX20_00528 at 544.261 kb on + strand, within MPMX20_00528 at 544.364 kb on + strand, within MPMX20_00528 at 544.372 kb on - strand, within MPMX20_00528 at 544.432 kb on + strand, within MPMX20_00528 at 544.441 kb on + strand, within MPMX20_00528 at 544.567 kb on + strand, within MPMX20_00528 at 544.567 kb on + strand, within MPMX20_00528 at 544.575 kb on - strand, within MPMX20_00528 at 544.577 kb on + strand, within MPMX20_00528 at 544.607 kb on + strand, within MPMX20_00528 at 544.658 kb on - strand, within MPMX20_00528 at 544.767 kb on - strand, within MPMX20_00528 at 544.781 kb on - strand, within MPMX20_00528
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, Mixed culture; Micromonospora sp. SAI_152 1:1 starding OD to Enterobacter_TBS_079_ML3; Collection-direct remove 541,792 - MPMX20_00525 0.86 -1.0 541,809 - MPMX20_00525 0.88 +0.6 541,809 - MPMX20_00525 0.88 -1.9 541,863 + -1.0 541,871 - -0.9 541,871 - -0.0 541,873 + +0.6 541,881 - -0.0 541,920 + -1.1 541,920 + +0.2 541,920 + -2.6 541,921 + -0.7 541,929 - -0.9 541,929 - -1.0 542,048 - MPMX20_00526 0.14 +0.7 542,117 - MPMX20_00526 0.23 +2.3 542,117 - MPMX20_00526 0.23 -1.1 542,120 - MPMX20_00526 0.24 +0.3 542,135 - MPMX20_00526 0.26 -0.3 542,169 + MPMX20_00526 0.30 +3.3 542,187 - MPMX20_00526 0.33 -0.3 542,224 + MPMX20_00526 0.38 +2.3 542,354 - MPMX20_00526 0.55 -0.2 542,361 + MPMX20_00526 0.56 -0.5 542,361 + MPMX20_00526 0.56 +0.4 542,369 - MPMX20_00526 0.57 -0.5 542,369 - MPMX20_00526 0.57 +2.3 542,369 - MPMX20_00526 0.57 -0.6 542,438 + MPMX20_00526 0.67 -1.3 542,446 - MPMX20_00526 0.68 -1.1 542,512 - MPMX20_00526 0.77 -1.3 542,532 + MPMX20_00526 0.79 -0.7 542,556 + MPMX20_00526 0.83 +2.3 542,556 + MPMX20_00526 0.83 +2.3 542,564 - MPMX20_00526 0.84 +2.3 542,585 + MPMX20_00526 0.87 +1.9 542,653 + -1.4 542,659 - -0.7 542,691 + -0.3 542,697 + -0.0 542,705 - +4.0 542,705 - -0.3 542,717 - +1.7 542,717 - -0.4 542,717 - -1.5 542,723 + -0.7 542,723 + +0.4 542,723 + +0.5 542,723 + +2.2 542,723 + -0.6 542,723 + -1.5 542,731 - +0.4 542,731 - +0.7 542,731 - -1.4 542,731 - -0.5 542,731 - -0.6 542,731 - -0.7 542,731 - -0.5 542,731 - -0.7 542,731 - -0.3 542,731 - +3.2 542,731 - -1.7 542,776 + -0.7 542,776 + -2.0 542,784 - -0.2 542,835 - -0.5 542,839 - +2.3 542,863 - -0.1 542,877 - +0.9 542,877 - -0.9 542,882 - +0.5 542,884 + MPMX20_00527 0.10 +0.7 542,930 + MPMX20_00527 0.14 -0.7 542,930 + MPMX20_00527 0.14 +2.3 542,938 - MPMX20_00527 0.15 +0.2 542,938 - MPMX20_00527 0.15 +2.2 542,942 + MPMX20_00527 0.15 +0.2 542,950 - MPMX20_00527 0.16 -1.0 542,950 - MPMX20_00527 0.16 +3.3 542,957 + MPMX20_00527 0.17 +2.3 543,001 + MPMX20_00527 0.20 +1.0 543,001 + MPMX20_00527 0.20 +2.3 543,009 - MPMX20_00527 0.21 +1.3 543,027 - MPMX20_00527 0.23 -0.8 543,029 + MPMX20_00527 0.23 +1.6 543,043 + MPMX20_00527 0.24 +1.3 543,043 + MPMX20_00527 0.24 -0.6 543,053 + MPMX20_00527 0.25 +2.3 543,066 - MPMX20_00527 0.26 -0.6 543,082 + MPMX20_00527 0.28 +0.8 543,090 - MPMX20_00527 0.28 +1.3 543,097 + MPMX20_00527 0.29 -0.1 543,117 + MPMX20_00527 0.31 +0.4 543,118 + MPMX20_00527 0.31 -0.5 543,125 - MPMX20_00527 0.31 +1.1 543,125 - MPMX20_00527 0.31 -0.7 543,195 - MPMX20_00527 0.38 -0.3 543,218 - MPMX20_00527 0.40 +0.7 543,307 - MPMX20_00527 0.47 -0.0 543,353 + MPMX20_00527 0.51 -0.3 543,366 - MPMX20_00527 0.53 +0.3 543,394 + MPMX20_00527 0.55 -0.5 543,521 + MPMX20_00527 0.66 +1.7 543,534 + MPMX20_00527 0.67 +1.8 543,542 - MPMX20_00527 0.68 +0.6 543,576 - MPMX20_00527 0.71 -1.0 543,583 - MPMX20_00527 0.72 +0.5 543,716 - MPMX20_00527 0.84 -1.0 543,733 + MPMX20_00527 0.85 -1.3 543,741 - MPMX20_00527 0.86 +0.6 543,756 - MPMX20_00527 0.87 +0.3 543,756 - MPMX20_00527 0.87 -0.2 543,775 + MPMX20_00527 0.89 +5.2 543,789 + +2.3 543,833 - +4.4 543,844 + -0.6 543,847 - +0.5 543,847 - -0.4 543,852 - -0.6 543,854 + +0.7 543,864 - -1.6 543,896 + -0.0 543,897 + -0.0 543,912 + +2.3 543,912 + -1.0 543,912 + -0.9 543,912 + +0.6 543,920 - +0.0 543,920 - +0.3 543,920 - -0.5 543,920 - -0.3 543,920 - -0.6 544,014 - -0.3 544,080 + -1.4 544,141 - MPMX20_00528 0.11 -1.4 544,151 + MPMX20_00528 0.12 -0.0 544,151 + MPMX20_00528 0.12 -1.3 544,151 + MPMX20_00528 0.12 -0.2 544,151 - MPMX20_00528 0.12 -1.0 544,160 + MPMX20_00528 0.12 -2.3 544,177 + MPMX20_00528 0.13 -2.9 544,259 - MPMX20_00528 0.18 -0.4 544,261 + MPMX20_00528 0.18 +0.8 544,364 + MPMX20_00528 0.23 -0.4 544,372 - MPMX20_00528 0.24 -1.5 544,432 + MPMX20_00528 0.27 -0.6 544,441 + MPMX20_00528 0.27 -0.8 544,567 + MPMX20_00528 0.34 -1.0 544,567 + MPMX20_00528 0.34 -0.7 544,575 - MPMX20_00528 0.34 +0.1 544,577 + MPMX20_00528 0.34 +0.3 544,607 + MPMX20_00528 0.36 -1.4 544,658 - MPMX20_00528 0.39 +2.3 544,767 - MPMX20_00528 0.44 -1.3 544,781 - MPMX20_00528 0.45 -1.2
Or see this region's nucleotide sequence