Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_431

Experiment: phage 93.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_429 and Ac3H11_430 are separated by 20 nucleotidesAc3H11_430 and Ac3H11_431 are separated by 9 nucleotidesAc3H11_431 and Ac3H11_432 are separated by 43 nucleotidesAc3H11_432 and Ac3H11_433 are separated by 21 nucleotides Ac3H11_429 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabZ form (EC 4.2.1.59), at 115,541 to 115,981 _429 Ac3H11_430 - Acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase (EC 2.3.1.129), at 116,002 to 116,790 _430 Ac3H11_431 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 116,800 to 117,963 _431 Ac3H11_432 - Ribonuclease HII (EC 3.1.26.4), at 118,007 to 118,555 _432 Ac3H11_433 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 118,577 to 119,350 _433 Position (kb) 116 117 118Strain fitness (log2 ratio) -1 0 1at 118.171 kb on + strand, within Ac3H11_432at 118.171 kb on + strand, within Ac3H11_432at 118.172 kb on - strand, within Ac3H11_432at 118.172 kb on - strand, within Ac3H11_432at 118.434 kb on - strand, within Ac3H11_432at 118.434 kb on - strand, within Ac3H11_432at 118.434 kb on - strand, within Ac3H11_432at 118.508 kb on + strandat 118.508 kb on + strandat 118.508 kb on + strandat 118.508 kb on + strandat 118.572 kb on + strandat 118.572 kb on + strandat 118.572 kb on + strandat 118.573 kb on - strandat 118.788 kb on - strand, within Ac3H11_433

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Per-strain Table

Position Strand Gene LocusTag Fraction phage 93.1
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118,171 + Ac3H11_432 0.30 +0.1
118,171 + Ac3H11_432 0.30 +0.7
118,172 - Ac3H11_432 0.30 +0.9
118,172 - Ac3H11_432 0.30 +1.5
118,434 - Ac3H11_432 0.78 +0.0
118,434 - Ac3H11_432 0.78 +0.6
118,434 - Ac3H11_432 0.78 +1.0
118,508 + -1.4
118,508 + +0.1
118,508 + +1.6
118,508 + +1.4
118,572 + +1.0
118,572 + +1.1
118,572 + +1.1
118,573 - -0.1
118,788 - Ac3H11_433 0.27 -0.2

Or see this region's nucleotide sequence