Experiment: phage 93.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_222 and Ac3H11_223 are separated by 14 nucleotides Ac3H11_223 and Ac3H11_224 are separated by 124 nucleotides Ac3H11_224 and Ac3H11_225 are separated by 181 nucleotides Ac3H11_225 and Ac3H11_226 are separated by 171 nucleotides
Ac3H11_222 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 36,032 to 37,144
_222
Ac3H11_223 - hypothetical protein, at 37,159 to 37,731
_223
Ac3H11_224 - Heavy metal RND efflux outer membrane protein, CzcC family, at 37,856 to 39,136
_224
Ac3H11_225 - Cobalt-zinc-cadmium resistance protein czcI precursor, at 39,318 to 39,674
_225
Ac3H11_226 - hemin ABC transporter ATP binding protein, at 39,846 to 40,691
_226
Position (kb)
37
38
39
40 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 36.968 kb on + strand, within Ac3H11_222 at 36.969 kb on - strand, within Ac3H11_222 at 36.969 kb on - strand, within Ac3H11_222 at 37.532 kb on + strand, within Ac3H11_223 at 37.532 kb on + strand, within Ac3H11_223 at 37.533 kb on - strand, within Ac3H11_223 at 37.533 kb on - strand, within Ac3H11_223 at 37.587 kb on + strand, within Ac3H11_223 at 37.587 kb on + strand, within Ac3H11_223 at 37.675 kb on + strand at 37.676 kb on - strand at 37.782 kb on + strand at 37.840 kb on + strand at 37.841 kb on - strand at 37.841 kb on - strand at 39.602 kb on - strand, within Ac3H11_225 at 39.602 kb on - strand, within Ac3H11_225 at 39.689 kb on + strand at 39.689 kb on + strand at 39.690 kb on - strand at 39.690 kb on - strand at 39.690 kb on - strand at 39.741 kb on + strand at 39.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phage 93.1 remove 36,968 + Ac3H11_222 0.84 +0.2 36,969 - Ac3H11_222 0.84 -0.1 36,969 - Ac3H11_222 0.84 -0.6 37,532 + Ac3H11_223 0.65 -0.9 37,532 + Ac3H11_223 0.65 -3.0 37,533 - Ac3H11_223 0.65 -1.8 37,533 - Ac3H11_223 0.65 -1.4 37,587 + Ac3H11_223 0.75 -0.5 37,587 + Ac3H11_223 0.75 -1.0 37,675 + +1.2 37,676 - +0.7 37,782 + +0.7 37,840 + -1.8 37,841 - -0.1 37,841 - -0.8 39,602 - Ac3H11_225 0.80 +0.5 39,602 - Ac3H11_225 0.80 +0.7 39,689 + +0.2 39,689 + +0.6 39,690 - -0.3 39,690 - -0.4 39,690 - -0.3 39,741 + +0.5 39,865 + +0.6
Or see this region's nucleotide sequence