Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_948 and Pf6N2E2_949 overlap by 1 nucleotides Pf6N2E2_949 and Pf6N2E2_950 are separated by 516 nucleotides
Pf6N2E2_948 - Multidrug efflux transporter MexF, at 1,115,446 to 1,118,637
_948
Pf6N2E2_949 - Outer membrane protein OprN, at 1,118,637 to 1,120,055
_949
Pf6N2E2_950 - Type IV secretory pathway, VirB4 components, at 1,120,572 to 1,123,322
_950
Position (kb)
1118
1119
1120
1121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1117.718 kb on + strand, within Pf6N2E2_948 at 1117.719 kb on - strand, within Pf6N2E2_948 at 1117.719 kb on - strand, within Pf6N2E2_948 at 1117.719 kb on - strand, within Pf6N2E2_948 at 1117.726 kb on + strand, within Pf6N2E2_948 at 1117.726 kb on + strand, within Pf6N2E2_948 at 1117.727 kb on - strand, within Pf6N2E2_948 at 1117.727 kb on - strand, within Pf6N2E2_948 at 1117.727 kb on - strand, within Pf6N2E2_948 at 1117.727 kb on - strand, within Pf6N2E2_948 at 1117.727 kb on - strand, within Pf6N2E2_948 at 1117.741 kb on + strand, within Pf6N2E2_948 at 1117.741 kb on + strand, within Pf6N2E2_948 at 1117.741 kb on + strand, within Pf6N2E2_948 at 1117.741 kb on + strand, within Pf6N2E2_948 at 1117.742 kb on - strand, within Pf6N2E2_948 at 1117.742 kb on - strand, within Pf6N2E2_948 at 1117.742 kb on - strand, within Pf6N2E2_948 at 1117.775 kb on - strand, within Pf6N2E2_948 at 1117.775 kb on - strand, within Pf6N2E2_948 at 1117.918 kb on - strand, within Pf6N2E2_948 at 1117.918 kb on - strand, within Pf6N2E2_948 at 1117.924 kb on + strand, within Pf6N2E2_948 at 1118.035 kb on - strand, within Pf6N2E2_948 at 1118.063 kb on - strand, within Pf6N2E2_948 at 1118.063 kb on - strand, within Pf6N2E2_948 at 1118.258 kb on + strand, within Pf6N2E2_948 at 1118.258 kb on + strand, within Pf6N2E2_948 at 1118.259 kb on - strand, within Pf6N2E2_948 at 1118.259 kb on - strand, within Pf6N2E2_948 at 1118.265 kb on - strand, within Pf6N2E2_948 at 1118.265 kb on - strand, within Pf6N2E2_948 at 1118.598 kb on + strand at 1118.598 kb on + strand at 1118.598 kb on + strand at 1118.602 kb on - strand at 1118.654 kb on - strand at 1118.709 kb on + strand at 1118.764 kb on - strand at 1118.813 kb on + strand, within Pf6N2E2_949 at 1118.814 kb on - strand, within Pf6N2E2_949 at 1118.814 kb on - strand, within Pf6N2E2_949 at 1119.117 kb on + strand, within Pf6N2E2_949 at 1119.117 kb on + strand, within Pf6N2E2_949 at 1119.117 kb on + strand, within Pf6N2E2_949 at 1119.395 kb on + strand, within Pf6N2E2_949 at 1119.659 kb on + strand, within Pf6N2E2_949 at 1119.659 kb on + strand, within Pf6N2E2_949 at 1119.660 kb on - strand, within Pf6N2E2_949 at 1119.741 kb on + strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.742 kb on - strand, within Pf6N2E2_949 at 1119.879 kb on + strand, within Pf6N2E2_949 at 1119.880 kb on - strand, within Pf6N2E2_949 at 1119.900 kb on + strand, within Pf6N2E2_949 at 1119.901 kb on - strand, within Pf6N2E2_949 at 1119.996 kb on + strand at 1119.997 kb on - strand at 1119.997 kb on - strand at 1119.998 kb on + strand at 1119.999 kb on - strand at 1119.999 kb on - strand at 1120.091 kb on + strand at 1120.112 kb on + strand at 1120.210 kb on - strand at 1120.215 kb on + strand at 1120.216 kb on - strand at 1120.303 kb on + strand at 1120.352 kb on + strand at 1120.353 kb on - strand at 1120.449 kb on + strand at 1120.449 kb on + strand at 1120.450 kb on - strand at 1120.550 kb on + strand at 1120.573 kb on + strand at 1120.573 kb on + strand at 1120.708 kb on + strand at 1120.774 kb on + strand at 1120.774 kb on + strand at 1120.774 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,117,718 + Pf6N2E2_948 0.71 +1.7 1,117,719 - Pf6N2E2_948 0.71 -2.5 1,117,719 - Pf6N2E2_948 0.71 -1.7 1,117,719 - Pf6N2E2_948 0.71 -1.7 1,117,726 + Pf6N2E2_948 0.71 +0.6 1,117,726 + Pf6N2E2_948 0.71 -1.3 1,117,727 - Pf6N2E2_948 0.71 -1.2 1,117,727 - Pf6N2E2_948 0.71 +0.1 1,117,727 - Pf6N2E2_948 0.71 -0.0 1,117,727 - Pf6N2E2_948 0.71 +0.4 1,117,727 - Pf6N2E2_948 0.71 +0.3 1,117,741 + Pf6N2E2_948 0.72 -1.1 1,117,741 + Pf6N2E2_948 0.72 -2.2 1,117,741 + Pf6N2E2_948 0.72 -0.3 1,117,741 + Pf6N2E2_948 0.72 +0.7 1,117,742 - Pf6N2E2_948 0.72 -0.5 1,117,742 - Pf6N2E2_948 0.72 -0.6 1,117,742 - Pf6N2E2_948 0.72 +0.6 1,117,775 - Pf6N2E2_948 0.73 +0.3 1,117,775 - Pf6N2E2_948 0.73 -0.7 1,117,918 - Pf6N2E2_948 0.77 +0.7 1,117,918 - Pf6N2E2_948 0.77 -0.3 1,117,924 + Pf6N2E2_948 0.78 -1.2 1,118,035 - Pf6N2E2_948 0.81 +0.1 1,118,063 - Pf6N2E2_948 0.82 -0.0 1,118,063 - Pf6N2E2_948 0.82 +1.1 1,118,258 + Pf6N2E2_948 0.88 -0.0 1,118,258 + Pf6N2E2_948 0.88 -0.6 1,118,259 - Pf6N2E2_948 0.88 -2.4 1,118,259 - Pf6N2E2_948 0.88 -0.6 1,118,265 - Pf6N2E2_948 0.88 -0.1 1,118,265 - Pf6N2E2_948 0.88 -0.6 1,118,598 + -0.5 1,118,598 + +2.6 1,118,598 + -0.1 1,118,602 - -0.7 1,118,654 - +1.4 1,118,709 + -0.9 1,118,764 - +0.5 1,118,813 + Pf6N2E2_949 0.12 -0.3 1,118,814 - Pf6N2E2_949 0.12 -0.7 1,118,814 - Pf6N2E2_949 0.12 -0.8 1,119,117 + Pf6N2E2_949 0.34 -0.5 1,119,117 + Pf6N2E2_949 0.34 -3.4 1,119,117 + Pf6N2E2_949 0.34 -1.4 1,119,395 + Pf6N2E2_949 0.53 +0.2 1,119,659 + Pf6N2E2_949 0.72 -0.2 1,119,659 + Pf6N2E2_949 0.72 -1.9 1,119,660 - Pf6N2E2_949 0.72 +0.5 1,119,741 + Pf6N2E2_949 0.78 -2.1 1,119,742 - Pf6N2E2_949 0.78 +0.5 1,119,742 - Pf6N2E2_949 0.78 +0.2 1,119,742 - Pf6N2E2_949 0.78 -0.8 1,119,742 - Pf6N2E2_949 0.78 -1.0 1,119,879 + Pf6N2E2_949 0.88 -0.7 1,119,880 - Pf6N2E2_949 0.88 +2.3 1,119,900 + Pf6N2E2_949 0.89 -1.5 1,119,901 - Pf6N2E2_949 0.89 -0.7 1,119,996 + +0.5 1,119,997 - -0.3 1,119,997 - +0.3 1,119,998 + +0.6 1,119,999 - -0.9 1,119,999 - -0.3 1,120,091 + +1.1 1,120,112 + +0.8 1,120,210 - -0.2 1,120,215 + +0.4 1,120,216 - -0.6 1,120,303 + +0.3 1,120,352 + +1.4 1,120,353 - +0.4 1,120,449 + -1.3 1,120,449 + -1.3 1,120,450 - -0.5 1,120,550 + -2.3 1,120,573 + +0.3 1,120,573 + -1.0 1,120,708 + +0.0 1,120,774 + -1.2 1,120,774 + +0.1 1,120,774 + +1.3
Or see this region's nucleotide sequence