Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_680 and Pf6N2E2_681 overlap by 4 nucleotides Pf6N2E2_681 and Pf6N2E2_682 are separated by 112 nucleotides Pf6N2E2_682 and Pf6N2E2_683 are separated by 203 nucleotides
Pf6N2E2_680 - D-amino-acid oxidase (EC 1.4.3.3), at 784,720 to 785,835
_680
Pf6N2E2_681 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 785,832 to 786,764
_681
Pf6N2E2_682 - Predicted regulator PutR for proline utilization, GntR family, at 786,877 to 787,548
_682
Pf6N2E2_683 - Probable MFS transporter, at 787,752 to 788,918
_683
Position (kb)
785
786
787 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.397 kb on - strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.523 kb on - strand, within Pf6N2E2_680 at 785.947 kb on + strand, within Pf6N2E2_681 at 785.947 kb on + strand, within Pf6N2E2_681 at 786.073 kb on + strand, within Pf6N2E2_681 at 786.074 kb on - strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.198 kb on + strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.211 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.408 kb on + strand, within Pf6N2E2_681 at 786.832 kb on - strand at 786.832 kb on - strand at 786.837 kb on + strand at 786.837 kb on + strand at 786.838 kb on - strand at 786.838 kb on - strand at 786.838 kb on - strand at 787.115 kb on + strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.246 kb on + strand, within Pf6N2E2_682 at 787.247 kb on - strand, within Pf6N2E2_682 at 787.585 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 785,092 + Pf6N2E2_680 0.33 +1.5 785,092 + Pf6N2E2_680 0.33 -0.4 785,092 + Pf6N2E2_680 0.33 +0.2 785,092 + Pf6N2E2_680 0.33 +2.0 785,092 + Pf6N2E2_680 0.33 -0.1 785,093 - Pf6N2E2_680 0.33 -0.0 785,093 - Pf6N2E2_680 0.33 -0.0 785,093 - Pf6N2E2_680 0.33 +0.1 785,093 - Pf6N2E2_680 0.33 +1.3 785,093 - Pf6N2E2_680 0.33 -0.7 785,093 - Pf6N2E2_680 0.33 -0.7 785,093 - Pf6N2E2_680 0.33 -1.0 785,094 + Pf6N2E2_680 0.34 +0.3 785,094 + Pf6N2E2_680 0.34 -0.8 785,095 - Pf6N2E2_680 0.34 +1.3 785,095 - Pf6N2E2_680 0.34 +0.0 785,142 - Pf6N2E2_680 0.38 -0.0 785,142 - Pf6N2E2_680 0.38 -1.0 785,397 - Pf6N2E2_680 0.61 -0.8 785,522 + Pf6N2E2_680 0.72 +1.1 785,522 + Pf6N2E2_680 0.72 +3.7 785,523 - Pf6N2E2_680 0.72 +1.8 785,947 + Pf6N2E2_681 0.12 -0.2 785,947 + Pf6N2E2_681 0.12 +0.3 786,073 + Pf6N2E2_681 0.26 +2.6 786,074 - Pf6N2E2_681 0.26 +0.3 786,093 + Pf6N2E2_681 0.28 -0.9 786,093 + Pf6N2E2_681 0.28 -1.7 786,198 + Pf6N2E2_681 0.39 -0.8 786,206 - Pf6N2E2_681 0.40 -2.9 786,206 - Pf6N2E2_681 0.40 -0.7 786,206 - Pf6N2E2_681 0.40 -3.1 786,211 + Pf6N2E2_681 0.41 -0.1 786,355 + Pf6N2E2_681 0.56 -0.8 786,355 + Pf6N2E2_681 0.56 +0.3 786,408 + Pf6N2E2_681 0.62 +1.1 786,832 - +0.7 786,832 - +0.3 786,837 + -0.2 786,837 + +0.6 786,838 - -0.2 786,838 - +1.0 786,838 - +0.2 787,115 + Pf6N2E2_682 0.35 -1.0 787,116 - Pf6N2E2_682 0.36 -0.9 787,116 - Pf6N2E2_682 0.36 +0.1 787,116 - Pf6N2E2_682 0.36 -1.5 787,160 - Pf6N2E2_682 0.42 +0.3 787,160 - Pf6N2E2_682 0.42 -0.9 787,160 - Pf6N2E2_682 0.42 +1.8 787,160 - Pf6N2E2_682 0.42 +0.7 787,246 + Pf6N2E2_682 0.55 +0.8 787,247 - Pf6N2E2_682 0.55 -1.6 787,585 + +0.3
Or see this region's nucleotide sequence