Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_616 and Pf6N2E2_617 are separated by 143 nucleotides Pf6N2E2_617 and Pf6N2E2_618 overlap by 4 nucleotides Pf6N2E2_618 and Pf6N2E2_619 overlap by 4 nucleotides
Pf6N2E2_616 - hypothetical protein, at 699,541 to 699,666
_616
Pf6N2E2_617 - Probable glutathione S-transferase, at 699,810 to 700,412
_617
Pf6N2E2_618 - Exonuclease SbcC, at 700,409 to 704,050
_618
Pf6N2E2_619 - Exonuclease SbcD, at 704,047 to 705,291
_619
Position (kb) 700
701
702
703
704
705 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 699.527 kb on + strand at 699.527 kb on + strand at 699.528 kb on - strand at 699.578 kb on - strand, within Pf6N2E2_616 at 699.812 kb on - strand at 700.076 kb on - strand, within Pf6N2E2_617 at 700.076 kb on - strand, within Pf6N2E2_617 at 700.081 kb on + strand, within Pf6N2E2_617 at 700.081 kb on + strand, within Pf6N2E2_617 at 700.082 kb on - strand, within Pf6N2E2_617 at 700.394 kb on - strand at 700.394 kb on - strand at 700.394 kb on - strand at 700.559 kb on + strand at 700.697 kb on + strand at 700.697 kb on + strand at 700.697 kb on + strand at 700.698 kb on - strand at 700.771 kb on + strand at 700.771 kb on + strand at 700.771 kb on + strand at 700.772 kb on - strand at 700.772 kb on - strand at 700.782 kb on - strand, within Pf6N2E2_618 at 700.833 kb on + strand, within Pf6N2E2_618 at 700.908 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.991 kb on + strand, within Pf6N2E2_618 at 700.991 kb on + strand, within Pf6N2E2_618 at 701.345 kb on + strand, within Pf6N2E2_618 at 701.346 kb on - strand, within Pf6N2E2_618 at 701.346 kb on - strand, within Pf6N2E2_618 at 702.090 kb on + strand, within Pf6N2E2_618 at 702.090 kb on + strand, within Pf6N2E2_618 at 702.207 kb on + strand, within Pf6N2E2_618 at 702.579 kb on + strand, within Pf6N2E2_618 at 702.580 kb on - strand, within Pf6N2E2_618 at 703.244 kb on + strand, within Pf6N2E2_618 at 703.461 kb on + strand, within Pf6N2E2_618 at 703.462 kb on - strand, within Pf6N2E2_618 at 703.611 kb on + strand, within Pf6N2E2_618 at 704.130 kb on - strand at 704.234 kb on + strand, within Pf6N2E2_619 at 704.624 kb on + strand, within Pf6N2E2_619 at 704.624 kb on + strand, within Pf6N2E2_619 at 704.625 kb on - strand, within Pf6N2E2_619 at 704.770 kb on - strand, within Pf6N2E2_619
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 699,527 + -0.2 699,527 + -0.4 699,528 - +0.1 699,578 - Pf6N2E2_616 0.29 -0.2 699,812 - -0.1 700,076 - Pf6N2E2_617 0.44 +0.8 700,076 - Pf6N2E2_617 0.44 -0.8 700,081 + Pf6N2E2_617 0.45 -1.2 700,081 + Pf6N2E2_617 0.45 -1.6 700,082 - Pf6N2E2_617 0.45 +0.9 700,394 - +1.1 700,394 - +0.4 700,394 - +0.4 700,559 + -1.1 700,697 + -0.5 700,697 + -1.2 700,697 + +1.4 700,698 - -1.5 700,771 + +0.5 700,771 + +1.0 700,771 + -1.3 700,772 - +0.7 700,772 - -1.0 700,782 - Pf6N2E2_618 0.10 -0.7 700,833 + Pf6N2E2_618 0.12 -1.4 700,908 + Pf6N2E2_618 0.14 +0.4 700,917 + Pf6N2E2_618 0.14 +0.2 700,917 + Pf6N2E2_618 0.14 -0.5 700,917 + Pf6N2E2_618 0.14 -0.6 700,917 + Pf6N2E2_618 0.14 +0.1 700,917 + Pf6N2E2_618 0.14 -0.8 700,918 - Pf6N2E2_618 0.14 +0.9 700,918 - Pf6N2E2_618 0.14 -1.5 700,918 - Pf6N2E2_618 0.14 -1.8 700,991 + Pf6N2E2_618 0.16 -0.5 700,991 + Pf6N2E2_618 0.16 +0.1 701,345 + Pf6N2E2_618 0.26 +0.2 701,346 - Pf6N2E2_618 0.26 +1.3 701,346 - Pf6N2E2_618 0.26 +1.0 702,090 + Pf6N2E2_618 0.46 +1.0 702,090 + Pf6N2E2_618 0.46 -1.1 702,207 + Pf6N2E2_618 0.49 -2.2 702,579 + Pf6N2E2_618 0.60 +0.2 702,580 - Pf6N2E2_618 0.60 -1.0 703,244 + Pf6N2E2_618 0.78 -1.0 703,461 + Pf6N2E2_618 0.84 +0.3 703,462 - Pf6N2E2_618 0.84 -1.2 703,611 + Pf6N2E2_618 0.88 +0.6 704,130 - -0.7 704,234 + Pf6N2E2_619 0.15 -3.1 704,624 + Pf6N2E2_619 0.46 +0.4 704,624 + Pf6N2E2_619 0.46 +0.0 704,625 - Pf6N2E2_619 0.46 +0.1 704,770 - Pf6N2E2_619 0.58 +0.0
Or see this region's nucleotide sequence