Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1695

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1692 and Pf6N2E2_1693 are separated by 16 nucleotidesPf6N2E2_1693 and Pf6N2E2_1694 are separated by 139 nucleotidesPf6N2E2_1694 and Pf6N2E2_1695 are separated by 59 nucleotidesPf6N2E2_1695 and Pf6N2E2_1696 are separated by 265 nucleotides Pf6N2E2_1692 - transporter, LysE family, at 2,001,577 to 2,002,218 _1692 Pf6N2E2_1693 - hypothetical protein, at 2,002,235 to 2,002,354 _1693 Pf6N2E2_1694 - FMN reductase (EC 1.5.1.29), at 2,002,494 to 2,003,057 _1694 Pf6N2E2_1695 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 2,003,117 to 2,004,304 _1695 Pf6N2E2_1696 - Alkanesulfonates-binding protein, at 2,004,570 to 2,005,418 _1696 Position (kb) 2003 2004 2005Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2002.460 kb on + strandat 2002.460 kb on + strandat 2002.460 kb on + strandat 2002.461 kb on - strandat 2002.461 kb on - strandat 2002.490 kb on + strandat 2002.490 kb on + strandat 2002.490 kb on + strandat 2002.491 kb on - strandat 2002.491 kb on - strandat 2002.491 kb on - strandat 2002.491 kb on - strandat 2002.491 kb on - strandat 2002.756 kb on - strand, within Pf6N2E2_1694at 2003.687 kb on - strand, within Pf6N2E2_1695at 2003.687 kb on - strand, within Pf6N2E2_1695at 2003.740 kb on - strand, within Pf6N2E2_1695at 2003.755 kb on - strand, within Pf6N2E2_1695at 2003.876 kb on + strand, within Pf6N2E2_1695at 2003.939 kb on + strand, within Pf6N2E2_1695at 2003.939 kb on + strand, within Pf6N2E2_1695at 2003.940 kb on - strand, within Pf6N2E2_1695at 2003.940 kb on - strand, within Pf6N2E2_1695at 2003.940 kb on - strand, within Pf6N2E2_1695at 2004.296 kb on + strandat 2004.296 kb on + strandat 2004.296 kb on + strandat 2004.297 kb on - strandat 2004.297 kb on - strandat 2004.297 kb on - strandat 2004.297 kb on - strandat 2004.302 kb on + strandat 2004.392 kb on - strandat 2004.392 kb on - strandat 2004.849 kb on + strand, within Pf6N2E2_1696at 2004.850 kb on - strand, within Pf6N2E2_1696at 2004.942 kb on + strand, within Pf6N2E2_1696at 2005.008 kb on + strand, within Pf6N2E2_1696at 2005.009 kb on - strand, within Pf6N2E2_1696

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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2,002,460 + +0.2
2,002,460 + +0.3
2,002,460 + -0.5
2,002,461 - -0.9
2,002,461 - +2.3
2,002,490 + +0.6
2,002,490 + -2.1
2,002,490 + +0.7
2,002,491 - +1.7
2,002,491 - -0.5
2,002,491 - -0.5
2,002,491 - +0.1
2,002,491 - +0.2
2,002,756 - Pf6N2E2_1694 0.46 -0.2
2,003,687 - Pf6N2E2_1695 0.48 +0.1
2,003,687 - Pf6N2E2_1695 0.48 -0.2
2,003,740 - Pf6N2E2_1695 0.52 -1.1
2,003,755 - Pf6N2E2_1695 0.54 -1.6
2,003,876 + Pf6N2E2_1695 0.64 -0.9
2,003,939 + Pf6N2E2_1695 0.69 -0.1
2,003,939 + Pf6N2E2_1695 0.69 -1.5
2,003,940 - Pf6N2E2_1695 0.69 +0.8
2,003,940 - Pf6N2E2_1695 0.69 -0.2
2,003,940 - Pf6N2E2_1695 0.69 -2.9
2,004,296 + -0.1
2,004,296 + -1.5
2,004,296 + -0.6
2,004,297 - -1.2
2,004,297 - +0.2
2,004,297 - -0.0
2,004,297 - +1.1
2,004,302 + -0.1
2,004,392 - +1.0
2,004,392 - +0.6
2,004,849 + Pf6N2E2_1696 0.33 +1.7
2,004,850 - Pf6N2E2_1696 0.33 +0.7
2,004,942 + Pf6N2E2_1696 0.44 +0.0
2,005,008 + Pf6N2E2_1696 0.52 -0.6
2,005,009 - Pf6N2E2_1696 0.52 -0.2

Or see this region's nucleotide sequence