Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_105

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_104 and Pf6N2E2_105 overlap by 4 nucleotidesPf6N2E2_105 and Pf6N2E2_106 are separated by 10 nucleotidesPf6N2E2_106 and Pf6N2E2_107 are separated by 10 nucleotides Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747 _104 Pf6N2E2_105 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 150,744 to 151,610 _105 Pf6N2E2_106 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 151,621 to 152,421 _106 Pf6N2E2_107 - Probable transmembrane protein, at 152,432 to 152,704 _107 Position (kb) 150 151 152Strain fitness (log2 ratio) -2 -1 0 1 2at 149.914 kb on + strand, within Pf6N2E2_104at 149.914 kb on + strand, within Pf6N2E2_104at 149.914 kb on + strand, within Pf6N2E2_104at 149.915 kb on - strand, within Pf6N2E2_104at 149.915 kb on - strand, within Pf6N2E2_104at 149.915 kb on - strand, within Pf6N2E2_104at 149.942 kb on + strand, within Pf6N2E2_104at 149.943 kb on - strand, within Pf6N2E2_104at 149.943 kb on - strand, within Pf6N2E2_104at 150.223 kb on + strand, within Pf6N2E2_104at 150.224 kb on - strand, within Pf6N2E2_104at 150.280 kb on + strand, within Pf6N2E2_104at 150.281 kb on - strand, within Pf6N2E2_104at 150.281 kb on - strand, within Pf6N2E2_104at 150.532 kb on + strand, within Pf6N2E2_104at 150.533 kb on - strand, within Pf6N2E2_104at 150.583 kb on + strand, within Pf6N2E2_104at 150.583 kb on + strand, within Pf6N2E2_104at 150.584 kb on - strand, within Pf6N2E2_104at 150.584 kb on - strand, within Pf6N2E2_104at 150.627 kb on - strand, within Pf6N2E2_104at 150.667 kb on + strandat 150.667 kb on + strandat 150.667 kb on + strandat 150.701 kb on - strandat 150.712 kb on + strandat 150.712 kb on + strandat 150.879 kb on + strand, within Pf6N2E2_105at 151.167 kb on + strand, within Pf6N2E2_105at 151.167 kb on + strand, within Pf6N2E2_105at 151.168 kb on - strand, within Pf6N2E2_105at 151.168 kb on - strand, within Pf6N2E2_105at 151.173 kb on + strand, within Pf6N2E2_105at 151.173 kb on + strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.258 kb on - strand, within Pf6N2E2_105at 151.287 kb on + strand, within Pf6N2E2_105at 151.288 kb on - strand, within Pf6N2E2_105at 151.288 kb on - strand, within Pf6N2E2_105at 151.290 kb on + strand, within Pf6N2E2_105at 151.290 kb on + strandat 151.290 kb on + strand, within Pf6N2E2_105at 151.291 kb on - strand, within Pf6N2E2_105at 151.413 kb on + strand, within Pf6N2E2_105at 151.414 kb on - strand, within Pf6N2E2_105at 151.540 kb on - strandat 151.540 kb on - strandat 151.540 kb on - strandat 151.540 kb on - strandat 151.575 kb on + strandat 151.575 kb on + strandat 151.654 kb on + strandat 151.655 kb on - strandat 151.655 kb on - strandat 151.655 kb on - strandat 151.681 kb on + strandat 151.695 kb on + strandat 151.695 kb on + strandat 151.696 kb on - strandat 151.768 kb on + strand, within Pf6N2E2_106at 151.768 kb on + strand, within Pf6N2E2_106at 151.768 kb on + strand, within Pf6N2E2_106at 151.769 kb on - strand, within Pf6N2E2_106at 151.798 kb on + strand, within Pf6N2E2_106at 151.823 kb on - strand, within Pf6N2E2_106at 151.825 kb on + strand, within Pf6N2E2_106at 151.826 kb on - strand, within Pf6N2E2_106at 151.826 kb on - strand, within Pf6N2E2_106at 151.984 kb on + strand, within Pf6N2E2_106at 151.984 kb on + strand, within Pf6N2E2_106at 151.985 kb on - strand, within Pf6N2E2_106at 151.985 kb on - strand, within Pf6N2E2_106at 152.117 kb on - strand, within Pf6N2E2_106

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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149,914 + Pf6N2E2_104 0.25 +1.6
149,914 + Pf6N2E2_104 0.25 -0.4
149,914 + Pf6N2E2_104 0.25 +0.5
149,915 - Pf6N2E2_104 0.25 -1.5
149,915 - Pf6N2E2_104 0.25 +1.2
149,915 - Pf6N2E2_104 0.25 -0.4
149,942 + Pf6N2E2_104 0.27 -0.0
149,943 - Pf6N2E2_104 0.27 +0.2
149,943 - Pf6N2E2_104 0.27 -0.1
150,223 + Pf6N2E2_104 0.53 +0.7
150,224 - Pf6N2E2_104 0.53 -0.0
150,280 + Pf6N2E2_104 0.58 +1.7
150,281 - Pf6N2E2_104 0.58 -0.8
150,281 - Pf6N2E2_104 0.58 -0.8
150,532 + Pf6N2E2_104 0.81 -1.2
150,533 - Pf6N2E2_104 0.81 +0.9
150,583 + Pf6N2E2_104 0.85 +0.2
150,583 + Pf6N2E2_104 0.85 +1.0
150,584 - Pf6N2E2_104 0.85 -1.2
150,584 - Pf6N2E2_104 0.85 +0.4
150,627 - Pf6N2E2_104 0.89 -1.2
150,667 + -1.2
150,667 + -0.1
150,667 + -0.1
150,701 - +0.5
150,712 + -0.4
150,712 + -0.4
150,879 + Pf6N2E2_105 0.16 -1.9
151,167 + Pf6N2E2_105 0.49 -0.4
151,167 + Pf6N2E2_105 0.49 +1.0
151,168 - Pf6N2E2_105 0.49 -0.8
151,168 - Pf6N2E2_105 0.49 +0.1
151,173 + Pf6N2E2_105 0.49 -1.9
151,173 + Pf6N2E2_105 0.49 -0.8
151,174 - Pf6N2E2_105 0.50 -0.2
151,174 - Pf6N2E2_105 0.50 +0.1
151,174 - Pf6N2E2_105 0.50 -1.8
151,258 - Pf6N2E2_105 0.59 -0.2
151,287 + Pf6N2E2_105 0.63 +0.3
151,288 - Pf6N2E2_105 0.63 -0.0
151,288 - Pf6N2E2_105 0.63 -1.2
151,290 + Pf6N2E2_105 0.63 -0.2
151,290 + +1.6
151,290 + Pf6N2E2_105 0.63 -0.5
151,291 - Pf6N2E2_105 0.63 +0.1
151,413 + Pf6N2E2_105 0.77 -1.0
151,414 - Pf6N2E2_105 0.77 -1.4
151,540 - +1.2
151,540 - +0.6
151,540 - -1.4
151,540 - +1.4
151,575 + +2.4
151,575 + +0.2
151,654 + +0.8
151,655 - +0.2
151,655 - +0.5
151,655 - -0.0
151,681 + -0.3
151,695 + +0.2
151,695 + -0.6
151,696 - -0.4
151,768 + Pf6N2E2_106 0.18 +0.4
151,768 + Pf6N2E2_106 0.18 -0.6
151,768 + Pf6N2E2_106 0.18 +1.0
151,769 - Pf6N2E2_106 0.18 -0.1
151,798 + Pf6N2E2_106 0.22 -0.2
151,823 - Pf6N2E2_106 0.25 -0.7
151,825 + Pf6N2E2_106 0.25 +1.9
151,826 - Pf6N2E2_106 0.26 +1.1
151,826 - Pf6N2E2_106 0.26 -1.0
151,984 + Pf6N2E2_106 0.45 -1.6
151,984 + Pf6N2E2_106 0.45 +0.6
151,985 - Pf6N2E2_106 0.45 -1.1
151,985 - Pf6N2E2_106 0.45 -2.2
152,117 - Pf6N2E2_106 0.62 +1.3

Or see this region's nucleotide sequence