Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_691 and Pf6N2E2_692 overlap by 4 nucleotides Pf6N2E2_692 and Pf6N2E2_693 are separated by 274 nucleotides Pf6N2E2_693 and Pf6N2E2_694 overlap by 6 nucleotides
Pf6N2E2_691 - D-galactarate permease, at 797,045 to 798,442
_691
Pf6N2E2_692 - Glucarate dehydratase (EC 4.2.1.40), at 798,439 to 799,713
_692
Pf6N2E2_693 - hypothetical protein, at 799,988 to 800,125
_693
Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415
_694
Position (kb) 798
799
800 Strain fitness (log2 ratio) -2
-1
0
1
2 at 797.444 kb on + strand, within Pf6N2E2_691 at 797.444 kb on + strand, within Pf6N2E2_691 at 797.444 kb on + strand, within Pf6N2E2_691 at 797.445 kb on - strand, within Pf6N2E2_691 at 797.445 kb on - strand, within Pf6N2E2_691 at 797.447 kb on + strand, within Pf6N2E2_691 at 797.463 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.951 kb on + strand, within Pf6N2E2_691 at 798.140 kb on + strand, within Pf6N2E2_691 at 798.269 kb on + strand, within Pf6N2E2_691 at 798.439 kb on - strand at 798.439 kb on - strand at 798.562 kb on + strand at 798.562 kb on + strand at 798.563 kb on - strand at 798.563 kb on - strand at 798.583 kb on + strand, within Pf6N2E2_692 at 798.672 kb on + strand, within Pf6N2E2_692 at 798.892 kb on + strand, within Pf6N2E2_692 at 798.991 kb on + strand, within Pf6N2E2_692 at 799.110 kb on + strand, within Pf6N2E2_692 at 799.110 kb on + strand, within Pf6N2E2_692 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.927 kb on - strand at 799.952 kb on + strand at 799.952 kb on + strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.132 kb on - strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.222 kb on + strand at 800.258 kb on + strand, within Pf6N2E2_694 at 800.273 kb on + strand, within Pf6N2E2_694 at 800.274 kb on - strand, within Pf6N2E2_694 at 800.309 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.480 kb on + strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 797,444 + Pf6N2E2_691 0.29 +0.9 797,444 + Pf6N2E2_691 0.29 +0.6 797,444 + Pf6N2E2_691 0.29 -1.7 797,445 - Pf6N2E2_691 0.29 -0.3 797,445 - Pf6N2E2_691 0.29 -0.5 797,447 + Pf6N2E2_691 0.29 +1.4 797,463 + Pf6N2E2_691 0.30 -0.4 797,840 + Pf6N2E2_691 0.57 +0.8 797,840 + Pf6N2E2_691 0.57 -0.5 797,840 + Pf6N2E2_691 0.57 +1.9 797,840 + Pf6N2E2_691 0.57 +0.3 797,840 + Pf6N2E2_691 0.57 -1.1 797,840 + Pf6N2E2_691 0.57 -1.8 797,840 + Pf6N2E2_691 0.57 -0.4 797,840 + Pf6N2E2_691 0.57 +1.5 797,841 - Pf6N2E2_691 0.57 -0.1 797,841 - Pf6N2E2_691 0.57 +0.3 797,841 - Pf6N2E2_691 0.57 +1.5 797,841 - Pf6N2E2_691 0.57 -1.0 797,841 - Pf6N2E2_691 0.57 -1.0 797,841 - Pf6N2E2_691 0.57 -0.6 797,841 - Pf6N2E2_691 0.57 +0.4 797,951 + Pf6N2E2_691 0.65 -0.3 798,140 + Pf6N2E2_691 0.78 -0.1 798,269 + Pf6N2E2_691 0.88 -0.5 798,439 - +0.3 798,439 - -0.7 798,562 + -0.8 798,562 + +2.1 798,563 - +1.0 798,563 - +0.4 798,583 + Pf6N2E2_692 0.11 -1.2 798,672 + Pf6N2E2_692 0.18 -0.1 798,892 + Pf6N2E2_692 0.36 -0.8 798,991 + Pf6N2E2_692 0.43 -0.5 799,110 + Pf6N2E2_692 0.53 +1.5 799,110 + Pf6N2E2_692 0.53 -1.0 799,168 + Pf6N2E2_692 0.57 +0.1 799,168 + Pf6N2E2_692 0.57 -1.4 799,927 - +0.9 799,952 + +0.2 799,952 + -1.8 799,953 - +0.2 799,953 - -1.1 799,953 - -0.9 799,990 - +0.5 799,990 - +1.1 799,990 - +0.7 800,055 - Pf6N2E2_693 0.49 -0.3 800,055 - Pf6N2E2_693 0.49 -1.5 800,055 - Pf6N2E2_693 0.49 +0.1 800,132 - +0.0 800,169 + +1.5 800,169 + +0.0 800,169 + +0.2 800,169 + +0.2 800,169 + +0.4 800,170 - +0.6 800,170 - -1.7 800,170 - +0.3 800,170 - +0.1 800,222 + +2.3 800,258 + Pf6N2E2_694 0.11 +0.7 800,273 + Pf6N2E2_694 0.12 +0.6 800,274 - Pf6N2E2_694 0.12 -0.7 800,309 + Pf6N2E2_694 0.15 +0.3 800,354 + Pf6N2E2_694 0.18 -0.2 800,354 + Pf6N2E2_694 0.18 +0.3 800,355 - Pf6N2E2_694 0.18 +0.8 800,355 - Pf6N2E2_694 0.18 -0.5 800,355 - Pf6N2E2_694 0.18 -0.5 800,355 - Pf6N2E2_694 0.18 +0.3 800,480 + Pf6N2E2_694 0.28 +0.8 800,481 - Pf6N2E2_694 0.28 -1.3 800,481 - Pf6N2E2_694 0.28 -0.8 800,481 - Pf6N2E2_694 0.28 -0.1 800,481 - Pf6N2E2_694 0.28 -0.5
Or see this region's nucleotide sequence