Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_641

Experiment: LB no stress 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotidesPf6N2E2_640 and Pf6N2E2_641 overlap by 34 nucleotidesPf6N2E2_641 and Pf6N2E2_642 are separated by 31 nucleotides Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442 _639 Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280 _640 Pf6N2E2_641 - Extracytoplasmic function (ECF) sigma factor VreI, at 732,247 to 732,822 _641 Pf6N2E2_642 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 732,854 to 733,801 _642 Position (kb) 732 733Strain fitness (log2 ratio) -2 -1 0 1 2at 731.495 kb on + strandat 731.528 kb on + strandat 731.528 kb on + strandat 731.529 kb on - strandat 731.540 kb on - strandat 731.540 kb on - strandat 731.572 kb on + strandat 731.573 kb on - strandat 731.573 kb on - strandat 731.803 kb on + strand, within Pf6N2E2_640at 731.803 kb on + strand, within Pf6N2E2_640at 731.804 kb on - strand, within Pf6N2E2_640at 731.882 kb on + strand, within Pf6N2E2_640at 731.975 kb on + strand, within Pf6N2E2_640at 731.975 kb on + strand, within Pf6N2E2_640at 731.976 kb on - strand, within Pf6N2E2_640at 732.228 kb on + strandat 732.229 kb on - strandat 732.253 kb on - strandat 732.325 kb on + strand, within Pf6N2E2_641at 732.326 kb on - strand, within Pf6N2E2_641at 732.326 kb on - strand, within Pf6N2E2_641at 732.401 kb on - strand, within Pf6N2E2_641at 732.401 kb on - strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.455 kb on - strand, within Pf6N2E2_641at 732.477 kb on + strand, within Pf6N2E2_641at 732.622 kb on + strand, within Pf6N2E2_641at 732.623 kb on - strand, within Pf6N2E2_641at 732.709 kb on - strand, within Pf6N2E2_641at 733.517 kb on + strand, within Pf6N2E2_642at 733.517 kb on + strand, within Pf6N2E2_642at 733.518 kb on - strand, within Pf6N2E2_642at 733.518 kb on - strand, within Pf6N2E2_642

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 24 hours
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731,495 + +1.1
731,528 + -0.1
731,528 + -1.0
731,529 - -0.1
731,540 - -0.3
731,540 - +0.3
731,572 + -1.0
731,573 - -1.9
731,573 - +0.3
731,803 + Pf6N2E2_640 0.24 -1.7
731,803 + Pf6N2E2_640 0.24 +0.5
731,804 - Pf6N2E2_640 0.25 -0.1
731,882 + Pf6N2E2_640 0.37 -0.1
731,975 + Pf6N2E2_640 0.52 -0.7
731,975 + Pf6N2E2_640 0.52 +0.5
731,976 - Pf6N2E2_640 0.52 +0.3
732,228 + -0.5
732,229 - -0.4
732,253 - -1.0
732,325 + Pf6N2E2_641 0.14 +0.0
732,326 - Pf6N2E2_641 0.14 -0.1
732,326 - Pf6N2E2_641 0.14 -0.7
732,401 - Pf6N2E2_641 0.27 +0.9
732,401 - Pf6N2E2_641 0.27 +1.2
732,454 + Pf6N2E2_641 0.36 +1.2
732,454 + Pf6N2E2_641 0.36 +1.5
732,454 + Pf6N2E2_641 0.36 +0.9
732,454 + Pf6N2E2_641 0.36 +0.0
732,454 + Pf6N2E2_641 0.36 +0.2
732,455 - Pf6N2E2_641 0.36 +0.7
732,477 + Pf6N2E2_641 0.40 +1.6
732,622 + Pf6N2E2_641 0.65 +0.0
732,623 - Pf6N2E2_641 0.65 -0.2
732,709 - Pf6N2E2_641 0.80 +1.8
733,517 + Pf6N2E2_642 0.70 -0.5
733,517 + Pf6N2E2_642 0.70 +0.5
733,518 - Pf6N2E2_642 0.70 -0.5
733,518 - Pf6N2E2_642 0.70 +0.6

Or see this region's nucleotide sequence