Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5803 and Pf6N2E2_5804 are separated by 54 nucleotides Pf6N2E2_5804 and Pf6N2E2_5805 overlap by 4 nucleotides Pf6N2E2_5805 and Pf6N2E2_5806 are separated by 45 nucleotides
Pf6N2E2_5803 - Allantoicase (EC 3.5.3.4), at 6,620,661 to 6,621,656
_5803
Pf6N2E2_5804 - 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase, at 6,621,711 to 6,622,226
_5804
Pf6N2E2_5805 - Uricase (urate oxidase) (EC 1.7.3.3), at 6,622,223 to 6,623,164
_5805
Pf6N2E2_5806 - hypothetical protein, at 6,623,210 to 6,623,386
_5806
Position (kb)
6621
6622
6623 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6620.896 kb on + strand, within Pf6N2E2_5803 at 6620.897 kb on - strand, within Pf6N2E2_5803 at 6621.169 kb on + strand, within Pf6N2E2_5803 at 6621.202 kb on + strand, within Pf6N2E2_5803 at 6621.206 kb on + strand, within Pf6N2E2_5803 at 6621.206 kb on + strand, within Pf6N2E2_5803 at 6621.207 kb on - strand, within Pf6N2E2_5803 at 6621.367 kb on + strand, within Pf6N2E2_5803 at 6621.368 kb on - strand, within Pf6N2E2_5803 at 6621.368 kb on - strand, within Pf6N2E2_5803 at 6621.371 kb on + strand, within Pf6N2E2_5803 at 6621.372 kb on - strand, within Pf6N2E2_5803 at 6621.639 kb on + strand at 6621.646 kb on + strand at 6621.646 kb on + strand at 6621.647 kb on - strand at 6621.647 kb on - strand at 6621.647 kb on - strand at 6621.673 kb on + strand at 6621.673 kb on + strand at 6621.673 kb on + strand at 6621.674 kb on - strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.840 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.878 kb on - strand, within Pf6N2E2_5804 at 6622.153 kb on + strand, within Pf6N2E2_5804 at 6622.154 kb on - strand, within Pf6N2E2_5804 at 6622.257 kb on + strand at 6622.288 kb on + strand at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.333 kb on - strand, within Pf6N2E2_5805 at 6622.435 kb on - strand, within Pf6N2E2_5805 at 6622.496 kb on + strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.522 kb on + strand, within Pf6N2E2_5805 at 6622.582 kb on - strand, within Pf6N2E2_5805 at 6622.599 kb on + strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.674 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 6,620,896 + Pf6N2E2_5803 0.24 +0.9 6,620,897 - Pf6N2E2_5803 0.24 -0.1 6,621,169 + Pf6N2E2_5803 0.51 +0.6 6,621,202 + Pf6N2E2_5803 0.54 +0.2 6,621,206 + Pf6N2E2_5803 0.55 -0.7 6,621,206 + Pf6N2E2_5803 0.55 +1.8 6,621,207 - Pf6N2E2_5803 0.55 -0.3 6,621,367 + Pf6N2E2_5803 0.71 -2.2 6,621,368 - Pf6N2E2_5803 0.71 -0.7 6,621,368 - Pf6N2E2_5803 0.71 +0.2 6,621,371 + Pf6N2E2_5803 0.71 +0.6 6,621,372 - Pf6N2E2_5803 0.71 +0.9 6,621,639 + -1.5 6,621,646 + -1.4 6,621,646 + +0.5 6,621,647 - -0.7 6,621,647 - -0.5 6,621,647 - -0.0 6,621,673 + -0.1 6,621,673 + +0.8 6,621,673 + +0.2 6,621,674 - -0.9 6,621,676 + +0.9 6,621,676 + +0.8 6,621,676 + +0.6 6,621,676 + +0.1 6,621,677 - +0.6 6,621,677 - -0.9 6,621,677 - +0.5 6,621,677 - -0.8 6,621,677 - -0.3 6,621,840 + Pf6N2E2_5804 0.25 -0.4 6,621,877 + Pf6N2E2_5804 0.32 -1.1 6,621,877 + Pf6N2E2_5804 0.32 -1.3 6,621,877 + Pf6N2E2_5804 0.32 +0.1 6,621,878 - Pf6N2E2_5804 0.32 -0.6 6,622,153 + Pf6N2E2_5804 0.86 -1.0 6,622,154 - Pf6N2E2_5804 0.86 -1.2 6,622,257 + -0.5 6,622,288 + +0.2 6,622,332 + Pf6N2E2_5805 0.12 +1.7 6,622,332 + Pf6N2E2_5805 0.12 +0.4 6,622,333 - Pf6N2E2_5805 0.12 -1.1 6,622,435 - Pf6N2E2_5805 0.23 +1.2 6,622,496 + Pf6N2E2_5805 0.29 +1.1 6,622,497 - Pf6N2E2_5805 0.29 -1.0 6,622,497 - Pf6N2E2_5805 0.29 -0.0 6,622,522 + Pf6N2E2_5805 0.32 -0.1 6,622,582 - Pf6N2E2_5805 0.38 +0.1 6,622,599 + Pf6N2E2_5805 0.40 +0.5 6,622,600 - Pf6N2E2_5805 0.40 +2.8 6,622,600 - Pf6N2E2_5805 0.40 +0.1 6,622,600 - Pf6N2E2_5805 0.40 -0.7 6,622,600 - Pf6N2E2_5805 0.40 +1.1 6,622,615 - Pf6N2E2_5805 0.42 -2.1 6,622,615 - Pf6N2E2_5805 0.42 -1.1 6,622,674 + Pf6N2E2_5805 0.48 -0.2 6,622,761 + Pf6N2E2_5805 0.57 +0.3 6,622,761 + Pf6N2E2_5805 0.57 +0.9 6,622,761 + Pf6N2E2_5805 0.57 +0.4 6,622,761 + Pf6N2E2_5805 0.57 -0.0 6,622,761 + Pf6N2E2_5805 0.57 +0.1 6,622,761 + Pf6N2E2_5805 0.57 -0.2 6,622,761 + Pf6N2E2_5805 0.57 -1.4 6,622,761 + Pf6N2E2_5805 0.57 -0.1 6,622,761 + Pf6N2E2_5805 0.57 -1.3 6,622,762 - Pf6N2E2_5805 0.57 -1.6 6,622,762 - Pf6N2E2_5805 0.57 -0.4 6,622,762 - Pf6N2E2_5805 0.57 +0.2 6,622,762 - Pf6N2E2_5805 0.57 -0.7 6,622,762 - Pf6N2E2_5805 0.57 +0.6 6,623,218 + +0.4 6,623,218 + +0.7 6,623,218 + +0.8 6,623,219 - +0.1
Or see this region's nucleotide sequence