Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3562 and Pf6N2E2_3563 are separated by 118 nucleotides Pf6N2E2_3563 and Pf6N2E2_3564 are separated by 136 nucleotides
Pf6N2E2_3562 - Lipase precursor (EC 3.1.1.3), at 4,153,459 to 4,154,349
_3562
Pf6N2E2_3563 - FMN-dependent NADH-azoreductase, at 4,154,468 to 4,155,106
_3563
Pf6N2E2_3564 - Glycine cleavage system transcriptional activator, at 4,155,243 to 4,156,184
_3564
Position (kb)
4154
4155
4156 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4153.610 kb on + strand, within Pf6N2E2_3562 at 4153.666 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.751 kb on + strand, within Pf6N2E2_3562 at 4153.873 kb on - strand, within Pf6N2E2_3562 at 4153.998 kb on - strand, within Pf6N2E2_3562 at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.288 kb on - strand at 4154.314 kb on + strand at 4154.315 kb on - strand at 4154.404 kb on + strand at 4154.404 kb on + strand at 4154.404 kb on + strand at 4154.404 kb on + strand at 4154.405 kb on - strand at 4154.448 kb on - strand at 4154.715 kb on + strand, within Pf6N2E2_3563 at 4154.925 kb on + strand, within Pf6N2E2_3563 at 4154.925 kb on + strand, within Pf6N2E2_3563 at 4154.967 kb on - strand, within Pf6N2E2_3563 at 4154.967 kb on - strand, within Pf6N2E2_3563 at 4155.100 kb on + strand at 4155.100 kb on + strand at 4155.101 kb on - strand at 4155.128 kb on + strand at 4155.144 kb on + strand at 4155.144 kb on + strand at 4155.144 kb on + strand at 4155.145 kb on - strand at 4155.193 kb on - strand at 4155.211 kb on - strand at 4155.418 kb on + strand, within Pf6N2E2_3564 at 4155.419 kb on - strand, within Pf6N2E2_3564 at 4155.589 kb on - strand, within Pf6N2E2_3564 at 4155.589 kb on - strand, within Pf6N2E2_3564 at 4155.708 kb on + strand, within Pf6N2E2_3564 at 4155.744 kb on + strand, within Pf6N2E2_3564 at 4155.744 kb on + strand, within Pf6N2E2_3564 at 4155.744 kb on + strand, within Pf6N2E2_3564 at 4155.745 kb on - strand, within Pf6N2E2_3564 at 4155.825 kb on + strand, within Pf6N2E2_3564 at 4155.825 kb on + strand, within Pf6N2E2_3564 at 4155.825 kb on + strand, within Pf6N2E2_3564 at 4155.826 kb on - strand, within Pf6N2E2_3564 at 4155.826 kb on - strand, within Pf6N2E2_3564 at 4155.901 kb on - strand, within Pf6N2E2_3564 at 4155.916 kb on - strand, within Pf6N2E2_3564 at 4155.916 kb on - strand, within Pf6N2E2_3564 at 4155.965 kb on + strand, within Pf6N2E2_3564 at 4155.965 kb on + strand, within Pf6N2E2_3564 at 4156.042 kb on - strand, within Pf6N2E2_3564
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 4,153,610 + Pf6N2E2_3562 0.17 -0.6 4,153,666 + Pf6N2E2_3562 0.23 -0.4 4,153,748 + Pf6N2E2_3562 0.32 +0.6 4,153,748 + Pf6N2E2_3562 0.32 -1.0 4,153,748 + Pf6N2E2_3562 0.32 +1.3 4,153,748 + Pf6N2E2_3562 0.32 -0.7 4,153,748 + Pf6N2E2_3562 0.32 +0.2 4,153,749 - Pf6N2E2_3562 0.33 +0.2 4,153,749 - Pf6N2E2_3562 0.33 -0.1 4,153,749 - Pf6N2E2_3562 0.33 +0.1 4,153,749 - Pf6N2E2_3562 0.33 -0.1 4,153,749 - Pf6N2E2_3562 0.33 -0.7 4,153,749 - Pf6N2E2_3562 0.33 +0.2 4,153,751 + Pf6N2E2_3562 0.33 +0.6 4,153,873 - Pf6N2E2_3562 0.46 -0.0 4,153,998 - Pf6N2E2_3562 0.60 +0.3 4,154,273 + -0.2 4,154,273 + +0.2 4,154,273 + +1.1 4,154,273 + -0.5 4,154,273 + -0.0 4,154,274 - -0.3 4,154,274 - -0.7 4,154,274 - +0.3 4,154,274 - +0.5 4,154,274 - -1.2 4,154,274 - +0.3 4,154,274 - +0.4 4,154,274 - -0.2 4,154,274 - +0.2 4,154,288 - +0.1 4,154,314 + -0.1 4,154,315 - -0.1 4,154,404 + +0.5 4,154,404 + +0.3 4,154,404 + -1.0 4,154,404 + +2.3 4,154,405 - -1.0 4,154,448 - -1.5 4,154,715 + Pf6N2E2_3563 0.39 +1.0 4,154,925 + Pf6N2E2_3563 0.72 +0.7 4,154,925 + Pf6N2E2_3563 0.72 +0.8 4,154,967 - Pf6N2E2_3563 0.78 -0.8 4,154,967 - Pf6N2E2_3563 0.78 +0.1 4,155,100 + +1.1 4,155,100 + +1.8 4,155,101 - -0.8 4,155,128 + +0.4 4,155,144 + -0.6 4,155,144 + +0.6 4,155,144 + -2.0 4,155,145 - -1.1 4,155,193 - +0.4 4,155,211 - +1.7 4,155,418 + Pf6N2E2_3564 0.19 -1.8 4,155,419 - Pf6N2E2_3564 0.19 +1.5 4,155,589 - Pf6N2E2_3564 0.37 -0.0 4,155,589 - Pf6N2E2_3564 0.37 -0.3 4,155,708 + Pf6N2E2_3564 0.49 +0.0 4,155,744 + Pf6N2E2_3564 0.53 +1.3 4,155,744 + Pf6N2E2_3564 0.53 +0.2 4,155,744 + Pf6N2E2_3564 0.53 -0.8 4,155,745 - Pf6N2E2_3564 0.53 +0.6 4,155,825 + Pf6N2E2_3564 0.62 +0.7 4,155,825 + Pf6N2E2_3564 0.62 -1.7 4,155,825 + Pf6N2E2_3564 0.62 -0.1 4,155,826 - Pf6N2E2_3564 0.62 +0.2 4,155,826 - Pf6N2E2_3564 0.62 -2.0 4,155,901 - Pf6N2E2_3564 0.70 -0.7 4,155,916 - Pf6N2E2_3564 0.71 -0.2 4,155,916 - Pf6N2E2_3564 0.71 +0.5 4,155,965 + Pf6N2E2_3564 0.77 +0.3 4,155,965 + Pf6N2E2_3564 0.77 -0.7 4,156,042 - Pf6N2E2_3564 0.85 -0.1
Or see this region's nucleotide sequence