Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_313 and Pf6N2E2_314 are separated by 40 nucleotides Pf6N2E2_314 and Pf6N2E2_315 are separated by 193 nucleotides Pf6N2E2_315 and Pf6N2E2_316 are separated by 294 nucleotides
Pf6N2E2_313 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 379,362 to 381,089
_313
Pf6N2E2_314 - Isochorismatase (EC 3.3.2.1), at 381,130 to 381,663
_314
Pf6N2E2_315 - probable transmembrane protein, at 381,857 to 382,717
_315
Pf6N2E2_316 - membrane protein, putative, at 383,012 to 383,977
_316
Position (kb) 381
382
383 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 381.039 kb on + strand at 381.039 kb on + strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.392 kb on - strand, within Pf6N2E2_314 at 381.661 kb on + strand at 381.682 kb on - strand at 381.682 kb on - strand at 381.691 kb on + strand at 381.885 kb on + strand at 381.885 kb on + strand at 381.886 kb on - strand at 381.886 kb on - strand at 382.042 kb on + strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.262 kb on - strand, within Pf6N2E2_315 at 382.359 kb on + strand, within Pf6N2E2_315 at 382.360 kb on - strand, within Pf6N2E2_315 at 382.360 kb on - strand, within Pf6N2E2_315 at 382.655 kb on + strand at 382.864 kb on + strand at 382.864 kb on + strand at 382.865 kb on - strand at 383.062 kb on - strand at 383.062 kb on - strand at 383.072 kb on + strand at 383.072 kb on + strand at 383.073 kb on - strand at 383.686 kb on - strand, within Pf6N2E2_316 at 383.715 kb on - strand, within Pf6N2E2_316
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 381,039 + +2.0 381,039 + +0.8 381,040 - -1.8 381,040 - +0.7 381,040 - +0.0 381,040 - +0.7 381,040 - +0.3 381,304 + Pf6N2E2_314 0.33 +0.9 381,304 + Pf6N2E2_314 0.33 +0.3 381,304 + Pf6N2E2_314 0.33 -0.1 381,304 + Pf6N2E2_314 0.33 +2.0 381,304 + Pf6N2E2_314 0.33 -0.7 381,305 - Pf6N2E2_314 0.33 +1.6 381,305 - Pf6N2E2_314 0.33 +0.8 381,305 - Pf6N2E2_314 0.33 +1.2 381,305 - Pf6N2E2_314 0.33 -0.5 381,392 - Pf6N2E2_314 0.49 +0.2 381,661 + -0.2 381,682 - +0.5 381,682 - +1.3 381,691 + -0.4 381,885 + +0.4 381,885 + -0.5 381,886 - +0.7 381,886 - -0.2 382,042 + Pf6N2E2_315 0.21 -1.0 382,043 - Pf6N2E2_315 0.22 -0.1 382,043 - Pf6N2E2_315 0.22 +1.5 382,043 - Pf6N2E2_315 0.22 +0.4 382,262 - Pf6N2E2_315 0.47 +0.8 382,359 + Pf6N2E2_315 0.58 +0.5 382,360 - Pf6N2E2_315 0.58 -0.4 382,360 - Pf6N2E2_315 0.58 +4.0 382,655 + +0.6 382,864 + -1.2 382,864 + +1.3 382,865 - +0.3 383,062 - -0.1 383,062 - +0.1 383,072 + +0.5 383,072 + -1.4 383,073 - +1.0 383,686 - Pf6N2E2_316 0.70 -1.0 383,715 - Pf6N2E2_316 0.73 +1.0
Or see this region's nucleotide sequence