Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1876

Experiment: LB no stress 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1875 and Pf6N2E2_1876 are separated by 44 nucleotidesPf6N2E2_1876 and Pf6N2E2_1877 are separated by 23 nucleotides Pf6N2E2_1875 - Protoporphyrinogen oxidase (EC 1.3.3.4), at 2,223,041 to 2,224,369 _1875 Pf6N2E2_1876 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,224,414 to 2,225,805 _1876 Pf6N2E2_1877 - putative transmembrane efflux protein., at 2,225,829 to 2,227,007 _1877 Position (kb) 2224 2225 2226Strain fitness (log2 ratio) -2 -1 0 1 2at 2223.573 kb on - strand, within Pf6N2E2_1875at 2223.759 kb on + strand, within Pf6N2E2_1875at 2224.249 kb on + strandat 2224.315 kb on - strandat 2224.396 kb on - strandat 2224.454 kb on - strandat 2224.542 kb on + strandat 2224.543 kb on - strandat 2224.543 kb on - strandat 2224.672 kb on + strand, within Pf6N2E2_1876at 2224.673 kb on - strand, within Pf6N2E2_1876at 2225.113 kb on - strand, within Pf6N2E2_1876at 2225.230 kb on - strand, within Pf6N2E2_1876at 2225.372 kb on - strand, within Pf6N2E2_1876at 2225.542 kb on - strand, within Pf6N2E2_1876at 2225.868 kb on - strandat 2225.870 kb on - strandat 2225.953 kb on - strand, within Pf6N2E2_1877at 2226.071 kb on - strand, within Pf6N2E2_1877at 2226.534 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.655 kb on - strand, within Pf6N2E2_1877

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 24 hours
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2,223,573 - Pf6N2E2_1875 0.40 +2.1
2,223,759 + Pf6N2E2_1875 0.54 +0.4
2,224,249 + -0.7
2,224,315 - +2.5
2,224,396 - -0.7
2,224,454 - -0.5
2,224,542 + -1.0
2,224,543 - +0.5
2,224,543 - -1.1
2,224,672 + Pf6N2E2_1876 0.19 -1.1
2,224,673 - Pf6N2E2_1876 0.19 -2.0
2,225,113 - Pf6N2E2_1876 0.50 +1.5
2,225,230 - Pf6N2E2_1876 0.59 -1.0
2,225,372 - Pf6N2E2_1876 0.69 -0.9
2,225,542 - Pf6N2E2_1876 0.81 +0.5
2,225,868 - -0.3
2,225,870 - +0.7
2,225,953 - Pf6N2E2_1877 0.11 -2.0
2,226,071 - Pf6N2E2_1877 0.21 -0.1
2,226,534 + Pf6N2E2_1877 0.60 +0.3
2,226,654 + Pf6N2E2_1877 0.70 -2.1
2,226,654 + Pf6N2E2_1877 0.70 +0.4
2,226,654 + Pf6N2E2_1877 0.70 +0.0
2,226,654 + Pf6N2E2_1877 0.70 +0.2
2,226,655 - Pf6N2E2_1877 0.70 -0.2

Or see this region's nucleotide sequence