Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1791 and Pf6N2E2_1792 overlap by 4 nucleotides Pf6N2E2_1792 and Pf6N2E2_1793 are separated by 86 nucleotides Pf6N2E2_1793 and Pf6N2E2_1794 are separated by 96 nucleotides Pf6N2E2_1794 and Pf6N2E2_1795 are separated by 34 nucleotides
Pf6N2E2_1791 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 2,120,365 to 2,121,387
_1791
Pf6N2E2_1792 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 2,121,384 to 2,122,175
_1792
Pf6N2E2_1793 - Inositol transport system sugar-binding protein, at 2,122,262 to 2,123,188
_1793
Pf6N2E2_1794 - FIG00553873: hypothetical protein, at 2,123,285 to 2,124,046
_1794
Pf6N2E2_1795 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 2,124,081 to 2,124,908
_1795
Position (kb)
2122
2123
2124 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2121.493 kb on + strand, within Pf6N2E2_1792 at 2121.564 kb on + strand, within Pf6N2E2_1792 at 2121.925 kb on - strand, within Pf6N2E2_1792 at 2121.925 kb on - strand, within Pf6N2E2_1792 at 2121.925 kb on - strand, within Pf6N2E2_1792 at 2122.195 kb on + strand at 2122.196 kb on - strand at 2122.256 kb on - strand at 2122.297 kb on - strand at 2122.376 kb on + strand, within Pf6N2E2_1793 at 2122.376 kb on + strand, within Pf6N2E2_1793 at 2122.376 kb on + strand, within Pf6N2E2_1793 at 2122.376 kb on + strand, within Pf6N2E2_1793 at 2122.377 kb on - strand, within Pf6N2E2_1793 at 2122.377 kb on - strand, within Pf6N2E2_1793 at 2122.483 kb on + strand, within Pf6N2E2_1793 at 2122.484 kb on - strand, within Pf6N2E2_1793 at 2122.484 kb on - strand, within Pf6N2E2_1793 at 2122.484 kb on - strand, within Pf6N2E2_1793 at 2122.584 kb on - strand, within Pf6N2E2_1793 at 2122.584 kb on - strand, within Pf6N2E2_1793 at 2122.676 kb on + strand, within Pf6N2E2_1793 at 2122.859 kb on - strand, within Pf6N2E2_1793 at 2123.180 kb on + strand at 2123.180 kb on + strand at 2123.180 kb on + strand at 2123.180 kb on + strand at 2123.181 kb on - strand at 2123.181 kb on - strand at 2123.187 kb on - strand at 2123.254 kb on + strand at 2123.254 kb on + strand at 2123.255 kb on - strand at 2123.287 kb on - strand at 2123.460 kb on + strand, within Pf6N2E2_1794 at 2123.460 kb on + strand, within Pf6N2E2_1794 at 2123.460 kb on + strand, within Pf6N2E2_1794 at 2123.460 kb on + strand, within Pf6N2E2_1794 at 2123.461 kb on - strand, within Pf6N2E2_1794 at 2123.461 kb on - strand, within Pf6N2E2_1794 at 2123.461 kb on - strand, within Pf6N2E2_1794 at 2123.461 kb on - strand, within Pf6N2E2_1794 at 2123.464 kb on + strand, within Pf6N2E2_1794 at 2123.464 kb on + strand, within Pf6N2E2_1794 at 2123.464 kb on + strand, within Pf6N2E2_1794 at 2123.464 kb on + strand, within Pf6N2E2_1794 at 2123.464 kb on + strand, within Pf6N2E2_1794 at 2123.464 kb on + strand, within Pf6N2E2_1794 at 2123.465 kb on - strand, within Pf6N2E2_1794 at 2123.465 kb on - strand, within Pf6N2E2_1794 at 2123.465 kb on - strand, within Pf6N2E2_1794 at 2123.465 kb on - strand, within Pf6N2E2_1794 at 2123.562 kb on + strand, within Pf6N2E2_1794 at 2123.563 kb on - strand, within Pf6N2E2_1794 at 2123.572 kb on - strand, within Pf6N2E2_1794 at 2123.611 kb on + strand, within Pf6N2E2_1794 at 2123.611 kb on + strand, within Pf6N2E2_1794 at 2123.612 kb on - strand, within Pf6N2E2_1794 at 2123.612 kb on - strand, within Pf6N2E2_1794 at 2123.612 kb on - strand, within Pf6N2E2_1794 at 2123.935 kb on - strand, within Pf6N2E2_1794 at 2123.935 kb on - strand, within Pf6N2E2_1794 at 2123.935 kb on - strand, within Pf6N2E2_1794
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,121,493 + Pf6N2E2_1792 0.14 +0.5 2,121,564 + Pf6N2E2_1792 0.23 +1.5 2,121,925 - Pf6N2E2_1792 0.68 -0.0 2,121,925 - Pf6N2E2_1792 0.68 -0.7 2,121,925 - Pf6N2E2_1792 0.68 +0.5 2,122,195 + -0.9 2,122,196 - -0.4 2,122,256 - +0.3 2,122,297 - -2.0 2,122,376 + Pf6N2E2_1793 0.12 -1.7 2,122,376 + Pf6N2E2_1793 0.12 -0.3 2,122,376 + Pf6N2E2_1793 0.12 +0.5 2,122,376 + Pf6N2E2_1793 0.12 -0.1 2,122,377 - Pf6N2E2_1793 0.12 -1.3 2,122,377 - Pf6N2E2_1793 0.12 -0.5 2,122,483 + Pf6N2E2_1793 0.24 -0.1 2,122,484 - Pf6N2E2_1793 0.24 +3.0 2,122,484 - Pf6N2E2_1793 0.24 -0.1 2,122,484 - Pf6N2E2_1793 0.24 -0.4 2,122,584 - Pf6N2E2_1793 0.35 -2.7 2,122,584 - Pf6N2E2_1793 0.35 -1.1 2,122,676 + Pf6N2E2_1793 0.45 -0.4 2,122,859 - Pf6N2E2_1793 0.64 +0.5 2,123,180 + +1.2 2,123,180 + -0.2 2,123,180 + -0.5 2,123,180 + -0.3 2,123,181 - -0.4 2,123,181 - +0.1 2,123,187 - +1.1 2,123,254 + -1.5 2,123,254 + -0.4 2,123,255 - +1.0 2,123,287 - -0.2 2,123,460 + Pf6N2E2_1794 0.23 -0.2 2,123,460 + Pf6N2E2_1794 0.23 +0.7 2,123,460 + Pf6N2E2_1794 0.23 +0.3 2,123,460 + Pf6N2E2_1794 0.23 +0.6 2,123,461 - Pf6N2E2_1794 0.23 -0.3 2,123,461 - Pf6N2E2_1794 0.23 +0.7 2,123,461 - Pf6N2E2_1794 0.23 -0.4 2,123,461 - Pf6N2E2_1794 0.23 -0.3 2,123,464 + Pf6N2E2_1794 0.23 -2.3 2,123,464 + Pf6N2E2_1794 0.23 +0.7 2,123,464 + Pf6N2E2_1794 0.23 +0.2 2,123,464 + Pf6N2E2_1794 0.23 +0.7 2,123,464 + Pf6N2E2_1794 0.23 -0.4 2,123,464 + Pf6N2E2_1794 0.23 +0.0 2,123,465 - Pf6N2E2_1794 0.24 +0.1 2,123,465 - Pf6N2E2_1794 0.24 +0.6 2,123,465 - Pf6N2E2_1794 0.24 -0.5 2,123,465 - Pf6N2E2_1794 0.24 -1.3 2,123,562 + Pf6N2E2_1794 0.36 -0.1 2,123,563 - Pf6N2E2_1794 0.36 +0.9 2,123,572 - Pf6N2E2_1794 0.38 +0.9 2,123,611 + Pf6N2E2_1794 0.43 +0.5 2,123,611 + Pf6N2E2_1794 0.43 -0.2 2,123,612 - Pf6N2E2_1794 0.43 +0.6 2,123,612 - Pf6N2E2_1794 0.43 -1.3 2,123,612 - Pf6N2E2_1794 0.43 -0.3 2,123,935 - Pf6N2E2_1794 0.85 +1.5 2,123,935 - Pf6N2E2_1794 0.85 +0.2 2,123,935 - Pf6N2E2_1794 0.85 +2.0
Or see this region's nucleotide sequence