Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1764 and Pf6N2E2_1765 are separated by 12 nucleotides Pf6N2E2_1765 and Pf6N2E2_1766 are separated by 96 nucleotides
Pf6N2E2_1764 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,084,266 to 2,085,774
_1764
Pf6N2E2_1765 - hypothetical protein, at 2,085,787 to 2,087,091
_1765
Pf6N2E2_1766 - Outer membrane transporter, OMPP1/FadL/TodX family, at 2,087,188 to 2,088,243
_1766
Position (kb)
2085
2086
2087
2088 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2084.995 kb on - strand, within Pf6N2E2_1764 at 2085.049 kb on + strand, within Pf6N2E2_1764 at 2085.050 kb on - strand, within Pf6N2E2_1764 at 2085.050 kb on - strand, within Pf6N2E2_1764 at 2085.050 kb on - strand, within Pf6N2E2_1764 at 2085.159 kb on + strand, within Pf6N2E2_1764 at 2085.160 kb on - strand, within Pf6N2E2_1764 at 2085.160 kb on - strand, within Pf6N2E2_1764 at 2085.240 kb on - strand, within Pf6N2E2_1764 at 2085.352 kb on + strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.421 kb on + strand, within Pf6N2E2_1764 at 2085.421 kb on + strand, within Pf6N2E2_1764 at 2085.421 kb on + strand, within Pf6N2E2_1764 at 2085.482 kb on + strand, within Pf6N2E2_1764 at 2085.482 kb on + strand, within Pf6N2E2_1764 at 2085.640 kb on - strand at 2085.675 kb on + strand at 2085.675 kb on + strand at 2085.902 kb on - strand at 2085.902 kb on - strand at 2086.210 kb on + strand, within Pf6N2E2_1765 at 2086.236 kb on + strand, within Pf6N2E2_1765 at 2086.236 kb on + strand, within Pf6N2E2_1765 at 2086.237 kb on - strand, within Pf6N2E2_1765 at 2086.312 kb on + strand, within Pf6N2E2_1765 at 2086.346 kb on + strand, within Pf6N2E2_1765 at 2086.347 kb on - strand, within Pf6N2E2_1765 at 2086.571 kb on + strand, within Pf6N2E2_1765 at 2086.579 kb on + strand, within Pf6N2E2_1765 at 2086.628 kb on - strand, within Pf6N2E2_1765 at 2086.637 kb on - strand, within Pf6N2E2_1765 at 2086.637 kb on - strand, within Pf6N2E2_1765 at 2086.639 kb on - strand, within Pf6N2E2_1765 at 2086.831 kb on + strand, within Pf6N2E2_1765 at 2086.832 kb on - strand, within Pf6N2E2_1765 at 2086.925 kb on - strand, within Pf6N2E2_1765 at 2086.925 kb on - strand, within Pf6N2E2_1765 at 2087.138 kb on + strand at 2087.184 kb on - strand at 2087.302 kb on + strand, within Pf6N2E2_1766 at 2087.302 kb on + strand, within Pf6N2E2_1766 at 2087.464 kb on + strand, within Pf6N2E2_1766 at 2087.464 kb on + strand, within Pf6N2E2_1766 at 2087.479 kb on + strand, within Pf6N2E2_1766 at 2087.480 kb on - strand, within Pf6N2E2_1766 at 2087.518 kb on + strand, within Pf6N2E2_1766 at 2087.521 kb on - strand, within Pf6N2E2_1766 at 2087.521 kb on - strand, within Pf6N2E2_1766 at 2087.578 kb on + strand, within Pf6N2E2_1766 at 2087.579 kb on - strand, within Pf6N2E2_1766 at 2087.647 kb on + strand, within Pf6N2E2_1766 at 2087.648 kb on - strand, within Pf6N2E2_1766 at 2087.702 kb on - strand, within Pf6N2E2_1766 at 2087.738 kb on - strand, within Pf6N2E2_1766 at 2087.973 kb on + strand, within Pf6N2E2_1766 at 2087.973 kb on + strand, within Pf6N2E2_1766 at 2087.973 kb on + strand, within Pf6N2E2_1766 at 2087.974 kb on - strand, within Pf6N2E2_1766 at 2088.031 kb on + strand, within Pf6N2E2_1766
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,084,995 - Pf6N2E2_1764 0.48 +1.5 2,085,049 + Pf6N2E2_1764 0.52 +1.5 2,085,050 - Pf6N2E2_1764 0.52 -0.3 2,085,050 - Pf6N2E2_1764 0.52 -1.9 2,085,050 - Pf6N2E2_1764 0.52 -0.9 2,085,159 + Pf6N2E2_1764 0.59 +1.0 2,085,160 - Pf6N2E2_1764 0.59 -0.2 2,085,160 - Pf6N2E2_1764 0.59 +0.9 2,085,240 - Pf6N2E2_1764 0.65 -0.3 2,085,352 + Pf6N2E2_1764 0.72 +0.4 2,085,353 - Pf6N2E2_1764 0.72 +2.8 2,085,353 - Pf6N2E2_1764 0.72 -0.3 2,085,353 - Pf6N2E2_1764 0.72 -1.2 2,085,353 - Pf6N2E2_1764 0.72 +0.5 2,085,421 + Pf6N2E2_1764 0.77 -2.9 2,085,421 + Pf6N2E2_1764 0.77 +1.5 2,085,421 + Pf6N2E2_1764 0.77 +0.9 2,085,482 + Pf6N2E2_1764 0.81 -0.3 2,085,482 + Pf6N2E2_1764 0.81 +0.2 2,085,640 - -0.5 2,085,675 + +0.1 2,085,675 + +0.6 2,085,902 - +0.3 2,085,902 - +1.2 2,086,210 + Pf6N2E2_1765 0.32 -0.1 2,086,236 + Pf6N2E2_1765 0.34 +2.2 2,086,236 + Pf6N2E2_1765 0.34 -1.4 2,086,237 - Pf6N2E2_1765 0.34 +3.8 2,086,312 + Pf6N2E2_1765 0.40 +0.1 2,086,346 + Pf6N2E2_1765 0.43 +0.7 2,086,347 - Pf6N2E2_1765 0.43 +0.5 2,086,571 + Pf6N2E2_1765 0.60 +1.0 2,086,579 + Pf6N2E2_1765 0.61 +0.9 2,086,628 - Pf6N2E2_1765 0.64 -0.7 2,086,637 - Pf6N2E2_1765 0.65 +0.1 2,086,637 - Pf6N2E2_1765 0.65 +0.6 2,086,639 - Pf6N2E2_1765 0.65 -0.1 2,086,831 + Pf6N2E2_1765 0.80 +0.5 2,086,832 - Pf6N2E2_1765 0.80 +1.5 2,086,925 - Pf6N2E2_1765 0.87 +0.5 2,086,925 - Pf6N2E2_1765 0.87 +1.1 2,087,138 + +0.1 2,087,184 - +0.6 2,087,302 + Pf6N2E2_1766 0.11 +0.1 2,087,302 + Pf6N2E2_1766 0.11 +1.0 2,087,464 + Pf6N2E2_1766 0.26 +0.8 2,087,464 + Pf6N2E2_1766 0.26 +0.1 2,087,479 + Pf6N2E2_1766 0.28 -1.3 2,087,480 - Pf6N2E2_1766 0.28 +1.2 2,087,518 + Pf6N2E2_1766 0.31 +0.0 2,087,521 - Pf6N2E2_1766 0.32 -0.0 2,087,521 - Pf6N2E2_1766 0.32 +0.2 2,087,578 + Pf6N2E2_1766 0.37 +0.3 2,087,579 - Pf6N2E2_1766 0.37 +1.2 2,087,647 + Pf6N2E2_1766 0.43 +0.7 2,087,648 - Pf6N2E2_1766 0.44 -0.3 2,087,702 - Pf6N2E2_1766 0.49 -1.3 2,087,738 - Pf6N2E2_1766 0.52 -0.1 2,087,973 + Pf6N2E2_1766 0.74 -0.1 2,087,973 + Pf6N2E2_1766 0.74 +1.0 2,087,973 + Pf6N2E2_1766 0.74 -0.4 2,087,974 - Pf6N2E2_1766 0.74 -0.5 2,088,031 + Pf6N2E2_1766 0.80 -0.7
Or see this region's nucleotide sequence