Experiment: H2 N-free with 10mM D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00755 and MMP_RS00760 are separated by 49 nucleotides MMP_RS00760 and MMP_RS00765 are separated by 72 nucleotides
MMP_RS00755: MMP_RS00755 - tyrosine decarboxylase MfnA, at 137,305 to 138,459
_RS00755
MMP_RS00760: MMP_RS00760 - branched-chain-amino-acid transaminase, at 138,509 to 139,372
_RS00760
MMP_RS00765: MMP_RS00765 - IMP dehydrogenase, at 139,445 to 140,947
_RS00765
Position (kb) 138
139
140 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 137.522 kb on - strand at 137.551 kb on + strand at 137.829 kb on - strand at 137.933 kb on - strand at 137.934 kb on + strand at 137.943 kb on - strand at 138.458 kb on + strand at 138.464 kb on - strand at 138.564 kb on - strand at 138.660 kb on - strand, within MMP_RS00760 at 138.753 kb on - strand, within MMP_RS00760 at 138.753 kb on - strand, within MMP_RS00760 at 138.754 kb on + strand, within MMP_RS00760 at 138.764 kb on - strand, within MMP_RS00760 at 138.765 kb on + strand, within MMP_RS00760 at 138.768 kb on - strand, within MMP_RS00760 at 138.769 kb on + strand, within MMP_RS00760 at 138.769 kb on + strand, within MMP_RS00760 at 138.895 kb on + strand, within MMP_RS00760 at 139.062 kb on - strand, within MMP_RS00760 at 139.072 kb on - strand, within MMP_RS00760 at 139.081 kb on - strand, within MMP_RS00760 at 139.082 kb on + strand, within MMP_RS00760 at 139.371 kb on + strand at 139.433 kb on - strand at 139.434 kb on + strand at 139.446 kb on - strand at 139.625 kb on - strand at 140.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-Alanine remove 137,522 - -1.1 137,551 + -1.3 137,829 - -1.4 137,933 - -0.9 137,934 + -1.8 137,943 - -0.2 138,458 + +0.3 138,464 - +0.8 138,564 - +0.5 138,660 - MMP_RS00760 0.17 -0.6 138,753 - MMP_RS00760 0.28 -0.4 138,753 - MMP_RS00760 0.28 +0.2 138,754 + MMP_RS00760 0.28 +0.1 138,764 - MMP_RS00760 0.30 -0.4 138,765 + MMP_RS00760 0.30 +0.9 138,768 - MMP_RS00760 0.30 -0.7 138,769 + MMP_RS00760 0.30 +0.4 138,769 + MMP_RS00760 0.30 -0.2 138,895 + MMP_RS00760 0.45 +0.9 139,062 - MMP_RS00760 0.64 -1.2 139,072 - MMP_RS00760 0.65 -0.4 139,081 - MMP_RS00760 0.66 +0.4 139,082 + MMP_RS00760 0.66 -0.3 139,371 + +0.8 139,433 - +1.0 139,434 + +0.3 139,446 - -0.1 139,625 - -4.0 140,291 + -0.3
Or see this region's nucleotide sequence