Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5928 and Pf6N2E2_5929 are separated by 294 nucleotides Pf6N2E2_5929 and Pf6N2E2_5930 are separated by 279 nucleotides Pf6N2E2_5930 and Pf6N2E2_5931 are separated by 95 nucleotides Pf6N2E2_5931 and Pf6N2E2_5932 overlap by 4 nucleotides
Pf6N2E2_5928 - ABC-type multidrug transport system, permease component, at 6,748,271 to 6,749,056
_5928
Pf6N2E2_5929 - FIG00954461: hypothetical protein, at 6,749,351 to 6,749,731
_5929
Pf6N2E2_5930 - Predicted hydrolase of the metallo-beta-lactamase superfamily, clustered with KDO2-Lipid A biosynthesis genes, at 6,750,011 to 6,752,257
_5930
Pf6N2E2_5931 - MotA/TolQ/ExbB proton channel family protein, at 6,752,353 to 6,752,988
_5931
Pf6N2E2_5932 - Biopolymer transport protein ExbD/TolR, at 6,752,985 to 6,753,413
_5932
Position (kb)
6750
6751
6752
6753 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6749.055 kb on - strand at 6749.106 kb on + strand at 6749.107 kb on - strand at 6749.107 kb on - strand at 6749.228 kb on + strand at 6749.228 kb on + strand at 6749.407 kb on - strand, within Pf6N2E2_5929 at 6749.407 kb on - strand, within Pf6N2E2_5929 at 6749.407 kb on - strand, within Pf6N2E2_5929 at 6749.407 kb on - strand, within Pf6N2E2_5929 at 6749.574 kb on + strand, within Pf6N2E2_5929 at 6749.574 kb on + strand, within Pf6N2E2_5929 at 6749.574 kb on + strand, within Pf6N2E2_5929 at 6749.575 kb on - strand, within Pf6N2E2_5929 at 6749.575 kb on - strand, within Pf6N2E2_5929 at 6749.575 kb on - strand, within Pf6N2E2_5929 at 6749.575 kb on - strand, within Pf6N2E2_5929 at 6749.575 kb on - strand, within Pf6N2E2_5929 at 6749.575 kb on - strand, within Pf6N2E2_5929 at 6749.575 kb on - strand, within Pf6N2E2_5929 at 6749.809 kb on + strand at 6749.809 kb on + strand at 6749.809 kb on + strand at 6749.809 kb on + strand at 6749.809 kb on + strand at 6749.810 kb on - strand at 6749.841 kb on + strand at 6749.842 kb on - strand at 6749.842 kb on - strand at 6749.842 kb on - strand at 6749.842 kb on - strand at 6749.866 kb on + strand at 6749.894 kb on - strand at 6749.905 kb on + strand at 6749.905 kb on + strand at 6749.906 kb on - strand at 6749.906 kb on - strand at 6749.906 kb on - strand at 6749.906 kb on - strand at 6749.906 kb on - strand at 6749.906 kb on - strand at 6749.918 kb on + strand at 6749.952 kb on + strand at 6749.978 kb on + strand at 6749.978 kb on + strand at 6749.978 kb on + strand at 6750.024 kb on + strand at 6750.138 kb on - strand at 6750.138 kb on - strand at 6750.372 kb on + strand, within Pf6N2E2_5930 at 6750.373 kb on - strand, within Pf6N2E2_5930 at 6750.449 kb on + strand, within Pf6N2E2_5930 at 6750.449 kb on + strand, within Pf6N2E2_5930 at 6750.450 kb on - strand, within Pf6N2E2_5930 at 6750.450 kb on - strand, within Pf6N2E2_5930 at 6750.722 kb on + strand, within Pf6N2E2_5930 at 6750.722 kb on + strand, within Pf6N2E2_5930 at 6750.722 kb on + strand, within Pf6N2E2_5930 at 6750.722 kb on + strand, within Pf6N2E2_5930 at 6750.722 kb on + strand, within Pf6N2E2_5930 at 6750.821 kb on + strand, within Pf6N2E2_5930 at 6751.018 kb on - strand, within Pf6N2E2_5930 at 6751.194 kb on + strand, within Pf6N2E2_5930 at 6751.195 kb on - strand, within Pf6N2E2_5930 at 6751.240 kb on + strand, within Pf6N2E2_5930 at 6751.241 kb on - strand, within Pf6N2E2_5930 at 6751.287 kb on + strand, within Pf6N2E2_5930 at 6751.287 kb on + strand, within Pf6N2E2_5930 at 6751.288 kb on - strand, within Pf6N2E2_5930 at 6751.419 kb on + strand, within Pf6N2E2_5930 at 6751.419 kb on + strand, within Pf6N2E2_5930 at 6751.420 kb on - strand, within Pf6N2E2_5930 at 6751.449 kb on + strand, within Pf6N2E2_5930 at 6751.449 kb on + strand, within Pf6N2E2_5930 at 6751.450 kb on - strand, within Pf6N2E2_5930 at 6751.556 kb on + strand, within Pf6N2E2_5930 at 6751.557 kb on - strand, within Pf6N2E2_5930 at 6752.130 kb on + strand at 6752.131 kb on - strand at 6752.132 kb on + strand at 6752.132 kb on + strand at 6752.132 kb on + strand at 6752.132 kb on + strand at 6752.132 kb on + strand at 6752.132 kb on + strand at 6752.132 kb on + strand at 6752.132 kb on + strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.133 kb on - strand at 6752.253 kb on - strand at 6752.670 kb on + strand, within Pf6N2E2_5931 at 6752.670 kb on + strand, within Pf6N2E2_5931
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 6,749,055 - -0.6 6,749,106 + +0.7 6,749,107 - +1.2 6,749,107 - -0.5 6,749,228 + +0.7 6,749,228 + -1.3 6,749,407 - Pf6N2E2_5929 0.15 -1.0 6,749,407 - Pf6N2E2_5929 0.15 -1.9 6,749,407 - Pf6N2E2_5929 0.15 -1.0 6,749,407 - Pf6N2E2_5929 0.15 -0.5 6,749,574 + Pf6N2E2_5929 0.59 -1.9 6,749,574 + Pf6N2E2_5929 0.59 +1.7 6,749,574 + Pf6N2E2_5929 0.59 -0.4 6,749,575 - Pf6N2E2_5929 0.59 -0.6 6,749,575 - Pf6N2E2_5929 0.59 -0.9 6,749,575 - Pf6N2E2_5929 0.59 -1.6 6,749,575 - Pf6N2E2_5929 0.59 -3.3 6,749,575 - Pf6N2E2_5929 0.59 -0.4 6,749,575 - Pf6N2E2_5929 0.59 -2.0 6,749,575 - Pf6N2E2_5929 0.59 -1.0 6,749,809 + -0.7 6,749,809 + -2.1 6,749,809 + -0.9 6,749,809 + -2.7 6,749,809 + -0.1 6,749,810 - +0.7 6,749,841 + -0.7 6,749,842 - -1.6 6,749,842 - -0.4 6,749,842 - -0.3 6,749,842 - -2.4 6,749,866 + -0.6 6,749,894 - +0.3 6,749,905 + +0.7 6,749,905 + -0.6 6,749,906 - +0.0 6,749,906 - -0.3 6,749,906 - +0.6 6,749,906 - +0.1 6,749,906 - +0.2 6,749,906 - -0.5 6,749,918 + -0.4 6,749,952 + -0.7 6,749,978 + -1.3 6,749,978 + -0.8 6,749,978 + +0.1 6,750,024 + -1.7 6,750,138 - -1.3 6,750,138 - +2.3 6,750,372 + Pf6N2E2_5930 0.16 -2.1 6,750,373 - Pf6N2E2_5930 0.16 +0.2 6,750,449 + Pf6N2E2_5930 0.19 +0.0 6,750,449 + Pf6N2E2_5930 0.19 -0.4 6,750,450 - Pf6N2E2_5930 0.20 -0.3 6,750,450 - Pf6N2E2_5930 0.20 +1.3 6,750,722 + Pf6N2E2_5930 0.32 +0.1 6,750,722 + Pf6N2E2_5930 0.32 -0.6 6,750,722 + Pf6N2E2_5930 0.32 -0.9 6,750,722 + Pf6N2E2_5930 0.32 -0.3 6,750,722 + Pf6N2E2_5930 0.32 +1.6 6,750,821 + Pf6N2E2_5930 0.36 -1.2 6,751,018 - Pf6N2E2_5930 0.45 -1.6 6,751,194 + Pf6N2E2_5930 0.53 -1.3 6,751,195 - Pf6N2E2_5930 0.53 +1.1 6,751,240 + Pf6N2E2_5930 0.55 +0.3 6,751,241 - Pf6N2E2_5930 0.55 -0.9 6,751,287 + Pf6N2E2_5930 0.57 -1.0 6,751,287 + Pf6N2E2_5930 0.57 +0.1 6,751,288 - Pf6N2E2_5930 0.57 -0.0 6,751,419 + Pf6N2E2_5930 0.63 +0.3 6,751,419 + Pf6N2E2_5930 0.63 -0.1 6,751,420 - Pf6N2E2_5930 0.63 -0.0 6,751,449 + Pf6N2E2_5930 0.64 -2.0 6,751,449 + Pf6N2E2_5930 0.64 +1.7 6,751,450 - Pf6N2E2_5930 0.64 +1.0 6,751,556 + Pf6N2E2_5930 0.69 -1.4 6,751,557 - Pf6N2E2_5930 0.69 -0.8 6,752,130 + +0.7 6,752,131 - +0.7 6,752,132 + +0.0 6,752,132 + +0.1 6,752,132 + -0.2 6,752,132 + +0.4 6,752,132 + +1.7 6,752,132 + +0.2 6,752,132 + -1.0 6,752,132 + +1.4 6,752,133 - -0.1 6,752,133 - +0.0 6,752,133 - -0.9 6,752,133 - +0.2 6,752,133 - -0.9 6,752,133 - -1.4 6,752,133 - -1.0 6,752,133 - -1.1 6,752,133 - +0.4 6,752,253 - +0.2 6,752,670 + Pf6N2E2_5931 0.50 -1.1 6,752,670 + Pf6N2E2_5931 0.50 -0.6
Or see this region's nucleotide sequence