Experiment: Hydroxide 0.05 M 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf6N2E2_5270 and Pf6N2E2_5271 overlap by 8 nucleotides Pf6N2E2_5271 and Pf6N2E2_5272 are separated by 99 nucleotides Pf6N2E2_5272 and Pf6N2E2_5273 are separated by 0 nucleotides Pf6N2E2_5273 and Pf6N2E2_5274 are separated by 20 nucleotides Pf6N2E2_5274 and Pf6N2E2_5275 are separated by 205 nucleotides  
        
        Pf6N2E2_5270 - Type III effector HopPmaJ, at 6,065,142 to 6,065,480 
         
        _5270 
         
        
        Pf6N2E2_5271 - FIG00953380: hypothetical protein, at 6,065,473 to 6,065,769 
         
        _5271 
         
        
        Pf6N2E2_5272 - Dihydroneopterin triphosphate epimerase, at 6,065,869 to 6,066,249 
         
        _5272 
         
        
        Pf6N2E2_5273 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 6,066,250 to 6,066,810 
         
        _5273 
         
        
        Pf6N2E2_5274 - FolM Alternative dihydrofolate reductase 1, at 6,066,831 to 6,067,541 
         
        _5274 
         
        
        Pf6N2E2_5275 - putative membrane protein, at 6,067,747 to 6,068,307 
         
        _5275 
         Position (kb)  
6066 
 
6067 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 6065.390 kb on - strand, within Pf6N2E2_5270 at 6065.448 kb on - strand at 6065.448 kb on - strand at 6065.465 kb on + strand at 6065.466 kb on - strand at 6065.466 kb on - strand at 6065.535 kb on - strand, within Pf6N2E2_5271 at 6065.555 kb on + strand, within Pf6N2E2_5271 at 6065.567 kb on + strand, within Pf6N2E2_5271 at 6065.631 kb on - strand, within Pf6N2E2_5271 at 6065.631 kb on - strand, within Pf6N2E2_5271 at 6065.631 kb on - strand, within Pf6N2E2_5271 at 6065.847 kb on + strand at 6065.870 kb on + strand at 6065.877 kb on + strand at 6066.071 kb on + strand, within Pf6N2E2_5272 at 6066.103 kb on + strand, within Pf6N2E2_5272 at 6066.103 kb on + strand, within Pf6N2E2_5272 at 6066.122 kb on + strand, within Pf6N2E2_5272 at 6066.122 kb on + strand, within Pf6N2E2_5272 at 6066.123 kb on - strand, within Pf6N2E2_5272 at 6066.251 kb on + strand at 6066.251 kb on + strand at 6066.251 kb on + strand at 6066.252 kb on - strand at 6066.252 kb on - strand at 6066.252 kb on - strand at 6066.252 kb on - strand at 6066.252 kb on - strand at 6066.540 kb on + strand, within Pf6N2E2_5273 at 6066.540 kb on + strand, within Pf6N2E2_5273 at 6066.540 kb on + strand, within Pf6N2E2_5273 at 6066.540 kb on + strand, within Pf6N2E2_5273 at 6066.540 kb on + strand, within Pf6N2E2_5273 at 6066.540 kb on + strand, within Pf6N2E2_5273 at 6066.540 kb on + strand, within Pf6N2E2_5273 at 6066.541 kb on - strand, within Pf6N2E2_5273 at 6066.541 kb on - strand, within Pf6N2E2_5273 at 6066.541 kb on - strand, within Pf6N2E2_5273 at 6066.541 kb on - strand, within Pf6N2E2_5273 at 6066.542 kb on + strand, within Pf6N2E2_5273 at 6066.542 kb on + strand, within Pf6N2E2_5273 at 6066.542 kb on + strand, within Pf6N2E2_5273 at 6066.542 kb on + strand, within Pf6N2E2_5273 at 6066.542 kb on + strand, within Pf6N2E2_5273 at 6066.543 kb on - strand, within Pf6N2E2_5273 at 6066.543 kb on - strand, within Pf6N2E2_5273 at 6066.543 kb on - strand, within Pf6N2E2_5273 at 6066.543 kb on - strand, within Pf6N2E2_5273 at 6066.543 kb on - strand, within Pf6N2E2_5273 at 6066.543 kb on - strand, within Pf6N2E2_5273 at 6066.543 kb on - strand, within Pf6N2E2_5273 at 6066.597 kb on - strand, within Pf6N2E2_5273 at 6066.597 kb on - strand, within Pf6N2E2_5273 at 6066.684 kb on - strand, within Pf6N2E2_5273 at 6066.684 kb on - strand, within Pf6N2E2_5273 at 6066.698 kb on + strand, within Pf6N2E2_5273 at 6066.698 kb on + strand, within Pf6N2E2_5273 at 6066.698 kb on + strand, within Pf6N2E2_5273 at 6066.699 kb on - strand, within Pf6N2E2_5273 at 6066.699 kb on - strand, within Pf6N2E2_5273 at 6066.699 kb on - strand, within Pf6N2E2_5273 at 6066.786 kb on - strand at 6066.832 kb on + strand at 6066.832 kb on + strand at 6066.832 kb on + strand at 6066.833 kb on - strand at 6066.833 kb on - strand at 6066.833 kb on - strand at 6066.833 kb on - strand at 6066.880 kb on + strand at 6066.880 kb on + strand at 6066.880 kb on + strand at 6066.880 kb on + strand at 6066.880 kb on + strand at 6066.880 kb on + strand at 6066.880 kb on + strand at 6066.881 kb on - strand at 6066.881 kb on - strand at 6066.881 kb on - strand at 6066.881 kb on - strand at 6066.899 kb on - strand at 6066.899 kb on - strand at 6066.913 kb on + strand, within Pf6N2E2_5274 at 6066.913 kb on + strand, within Pf6N2E2_5274 at 6066.913 kb on + strand, within Pf6N2E2_5274 at 6066.913 kb on + strand, within Pf6N2E2_5274 at 6066.914 kb on - strand, within Pf6N2E2_5274 at 6067.002 kb on + strand, within Pf6N2E2_5274 at 6067.002 kb on + strand, within Pf6N2E2_5274 at 6067.003 kb on - strand, within Pf6N2E2_5274 at 6067.003 kb on - strand, within Pf6N2E2_5274 at 6067.003 kb on - strand, within Pf6N2E2_5274 at 6067.100 kb on - strand, within Pf6N2E2_5274 at 6067.180 kb on - strand, within Pf6N2E2_5274 at 6067.180 kb on - strand, within Pf6N2E2_5274 at 6067.180 kb on - strand, within Pf6N2E2_5274 at 6067.208 kb on - strand, within Pf6N2E2_5274 at 6067.208 kb on - strand, within Pf6N2E2_5274 at 6067.296 kb on + strand, within Pf6N2E2_5274 at 6067.296 kb on + strand, within Pf6N2E2_5274 at 6067.297 kb on - strand, within Pf6N2E2_5274 at 6067.297 kb on - strand, within Pf6N2E2_5274 at 6067.297 kb on - strand, within Pf6N2E2_5274 at 6067.297 kb on - strand, within Pf6N2E2_5274 at 6067.297 kb on - strand, within Pf6N2E2_5274 at 6067.436 kb on - strand, within Pf6N2E2_5274 at 6067.541 kb on + strand at 6067.541 kb on + strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.563 kb on - strand at 6067.573 kb on + strand at 6067.573 kb on + strand at 6067.573 kb on + strand at 6067.573 kb on + strand at 6067.574 kb on - strand at 6067.574 kb on - strand at 6067.574 kb on - strand at 6067.574 kb on - strand at 6067.576 kb on - strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.661 kb on + strand at 6067.662 kb on - strand at 6067.662 kb on - strand at 6067.662 kb on - strand at 6067.668 kb on + strand at 6067.668 kb on + strand at 6067.668 kb on + strand at 6067.669 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Hydroxide 0.05 M             remove   6,065,390   -    Pf6N2E2_5270   0.73  +0.2   6,065,448   -        -0.6   6,065,448   -        +0.2   6,065,465   +        -1.0   6,065,466   -        -0.0   6,065,466   -        +0.6   6,065,535   -    Pf6N2E2_5271   0.21  -0.6   6,065,555   +    Pf6N2E2_5271   0.28  +0.4   6,065,567   +    Pf6N2E2_5271   0.32  -1.4   6,065,631   -    Pf6N2E2_5271   0.53  +0.4   6,065,631   -    Pf6N2E2_5271   0.53  +0.7   6,065,631   -    Pf6N2E2_5271   0.53  -0.9   6,065,847   +        +0.7   6,065,870   +        -1.8   6,065,877   +        -0.1   6,066,071   +    Pf6N2E2_5272   0.53  +0.7   6,066,103   +    Pf6N2E2_5272   0.61  -0.2   6,066,103   +    Pf6N2E2_5272   0.61  +0.7   6,066,122   +    Pf6N2E2_5272   0.66  -0.1   6,066,122   +    Pf6N2E2_5272   0.66  +1.1   6,066,123   -    Pf6N2E2_5272   0.67  +0.2   6,066,251   +        +0.9   6,066,251   +        +0.2   6,066,251   +        +0.6   6,066,252   -        +0.8   6,066,252   -        +1.1   6,066,252   -        +1.3   6,066,252   -        +2.1   6,066,252   -        -0.5   6,066,540   +    Pf6N2E2_5273   0.52  +0.9   6,066,540   +    Pf6N2E2_5273   0.52  -0.7   6,066,540   +    Pf6N2E2_5273   0.52  +1.1   6,066,540   +    Pf6N2E2_5273   0.52  -0.2   6,066,540   +    Pf6N2E2_5273   0.52  -0.6   6,066,540   +    Pf6N2E2_5273   0.52  -0.4   6,066,540   +    Pf6N2E2_5273   0.52  +0.5   6,066,541   -    Pf6N2E2_5273   0.52  -0.6   6,066,541   -    Pf6N2E2_5273   0.52  -0.4   6,066,541   -    Pf6N2E2_5273   0.52  -0.1   6,066,541   -    Pf6N2E2_5273   0.52  -0.2   6,066,542   +    Pf6N2E2_5273   0.52  +0.9   6,066,542   +    Pf6N2E2_5273   0.52  +0.4   6,066,542   +    Pf6N2E2_5273   0.52  +1.3   6,066,542   +    Pf6N2E2_5273   0.52  -0.6   6,066,542   +    Pf6N2E2_5273   0.52  -0.9   6,066,543   -    Pf6N2E2_5273   0.52  -0.4   6,066,543   -    Pf6N2E2_5273   0.52  +0.2   6,066,543   -    Pf6N2E2_5273   0.52  -0.7   6,066,543   -    Pf6N2E2_5273   0.52  -0.2   6,066,543   -    Pf6N2E2_5273   0.52  -0.8   6,066,543   -    Pf6N2E2_5273   0.52  -1.5   6,066,543   -    Pf6N2E2_5273   0.52  +0.3   6,066,597   -    Pf6N2E2_5273   0.62  +0.6   6,066,597   -    Pf6N2E2_5273   0.62  -1.2   6,066,684   -    Pf6N2E2_5273   0.77  -1.0   6,066,684   -    Pf6N2E2_5273   0.77  -0.2   6,066,698   +    Pf6N2E2_5273   0.80  +0.7   6,066,698   +    Pf6N2E2_5273   0.80  -0.1   6,066,698   +    Pf6N2E2_5273   0.80  +0.4   6,066,699   -    Pf6N2E2_5273   0.80  +0.4   6,066,699   -    Pf6N2E2_5273   0.80  -0.6   6,066,699   -    Pf6N2E2_5273   0.80  -1.1   6,066,786   -        -0.8   6,066,832   +        +0.6   6,066,832   +        +0.3   6,066,832   +        -0.1   6,066,833   -        +0.4   6,066,833   -        +0.8   6,066,833   -        +0.6   6,066,833   -        +0.1   6,066,880   +        -0.7   6,066,880   +        +0.9   6,066,880   +        +0.3   6,066,880   +        +1.0   6,066,880   +        +0.6   6,066,880   +        +0.0   6,066,880   +        +0.3   6,066,881   -        -0.5   6,066,881   -        -1.1   6,066,881   -        -0.5   6,066,881   -        +0.9   6,066,899   -        -0.7   6,066,899   -        +1.5   6,066,913   +    Pf6N2E2_5274   0.12  -0.4   6,066,913   +    Pf6N2E2_5274   0.12  +0.0   6,066,913   +    Pf6N2E2_5274   0.12  +0.2   6,066,913   +    Pf6N2E2_5274   0.12  -0.3   6,066,914   -    Pf6N2E2_5274   0.12  +0.9   6,067,002   +    Pf6N2E2_5274   0.24  -0.3   6,067,002   +    Pf6N2E2_5274   0.24  -0.0   6,067,003   -    Pf6N2E2_5274   0.24  -0.5   6,067,003   -    Pf6N2E2_5274   0.24  -0.7   6,067,003   -    Pf6N2E2_5274   0.24  +0.4   6,067,100   -    Pf6N2E2_5274   0.38  +0.5   6,067,180   -    Pf6N2E2_5274   0.49  +0.4   6,067,180   -    Pf6N2E2_5274   0.49  -0.7   6,067,180   -    Pf6N2E2_5274   0.49  +0.8   6,067,208   -    Pf6N2E2_5274   0.53  +0.6   6,067,208   -    Pf6N2E2_5274   0.53  -0.4   6,067,296   +    Pf6N2E2_5274   0.65  -2.0   6,067,296   +    Pf6N2E2_5274   0.65  -0.2   6,067,297   -    Pf6N2E2_5274   0.66  -1.2   6,067,297   -    Pf6N2E2_5274   0.66  +0.7   6,067,297   -    Pf6N2E2_5274   0.66  -0.4   6,067,297   -    Pf6N2E2_5274   0.66  +0.8   6,067,297   -    Pf6N2E2_5274   0.66  -2.4   6,067,436   -    Pf6N2E2_5274   0.85  -0.9   6,067,541   +        -0.7   6,067,541   +        -0.7   6,067,542   -        -0.0   6,067,542   -        -0.2   6,067,542   -        -0.3   6,067,542   -        -0.3   6,067,542   -        -0.1   6,067,542   -        +0.5   6,067,542   -        -2.2   6,067,563   -        +0.6   6,067,573   +        +0.2   6,067,573   +        -1.5   6,067,573   +        -1.2   6,067,573   +        +0.8   6,067,574   -        -0.7   6,067,574   -        +0.6   6,067,574   -        -1.4   6,067,574   -        -0.7   6,067,576   -        -0.6   6,067,598   +        -0.7   6,067,598   +        +0.7   6,067,598   +        +0.3   6,067,598   +        -1.7   6,067,598   +        +0.4   6,067,598   +        -2.1   6,067,598   +        -0.2   6,067,598   +        +1.0   6,067,598   +        +0.1   6,067,599   -        +0.2   6,067,599   -        +1.5   6,067,599   -        +1.2   6,067,599   -        +1.1   6,067,599   -        -1.3   6,067,599   -        -0.2   6,067,599   -        +0.1   6,067,599   -        +0.5   6,067,599   -        -0.6   6,067,599   -        +0.6   6,067,599   -        +0.7   6,067,599   -        -0.7   6,067,602   +        +1.3   6,067,602   +        +0.5   6,067,602   +        +1.5   6,067,602   +        +0.0   6,067,602   +        +0.3   6,067,602   +        -0.6   6,067,603   -        -0.4   6,067,603   -        -0.3   6,067,603   -        +0.4   6,067,603   -        +1.3   6,067,603   -        +0.9   6,067,661   +        +0.3   6,067,662   -        +0.1   6,067,662   -        -0.9   6,067,662   -        +0.1   6,067,668   +        +0.8   6,067,668   +        +0.5   6,067,668   +        +1.2   6,067,669   -        +0.4 
 
Or see this region's nucleotide sequence