Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4156 and Pf6N2E2_4157 overlap by 4 nucleotides Pf6N2E2_4157 and Pf6N2E2_4158 are separated by 15 nucleotides Pf6N2E2_4158 and Pf6N2E2_4159 are separated by 198 nucleotides
Pf6N2E2_4156 - GGDEF domain protein, at 4,837,365 to 4,839,431
_4156
Pf6N2E2_4157 - Endonuclease/exonuclease/phosphatase family protein, at 4,839,428 to 4,840,306
_4157
Pf6N2E2_4158 - Periplasmic thiol:disulfide interchange protein DsbA, at 4,840,322 to 4,840,963
_4158
Pf6N2E2_4159 - Cytochrome c4, at 4,841,162 to 4,841,773
_4159
Position (kb)
4839
4840
4841 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4838.437 kb on + strand, within Pf6N2E2_4156 at 4838.437 kb on + strand, within Pf6N2E2_4156 at 4838.577 kb on + strand, within Pf6N2E2_4156 at 4838.578 kb on - strand, within Pf6N2E2_4156 at 4838.578 kb on - strand, within Pf6N2E2_4156 at 4839.186 kb on + strand, within Pf6N2E2_4156 at 4839.387 kb on - strand at 4839.397 kb on + strand at 4839.397 kb on + strand at 4839.397 kb on + strand at 4839.397 kb on + strand at 4839.398 kb on - strand at 4839.398 kb on - strand at 4839.398 kb on - strand at 4839.398 kb on - strand at 4839.398 kb on - strand at 4839.398 kb on - strand at 4839.753 kb on + strand, within Pf6N2E2_4157 at 4839.754 kb on - strand, within Pf6N2E2_4157 at 4839.967 kb on - strand, within Pf6N2E2_4157 at 4839.967 kb on - strand, within Pf6N2E2_4157 at 4839.967 kb on - strand, within Pf6N2E2_4157 at 4840.017 kb on + strand, within Pf6N2E2_4157 at 4840.017 kb on + strand, within Pf6N2E2_4157 at 4840.152 kb on + strand, within Pf6N2E2_4157 at 4840.152 kb on + strand, within Pf6N2E2_4157 at 4840.152 kb on + strand, within Pf6N2E2_4157 at 4840.153 kb on - strand, within Pf6N2E2_4157 at 4840.153 kb on - strand, within Pf6N2E2_4157 at 4840.323 kb on + strand at 4840.323 kb on + strand at 4840.323 kb on + strand at 4840.323 kb on + strand at 4840.323 kb on + strand at 4840.324 kb on - strand at 4840.324 kb on - strand at 4840.324 kb on - strand at 4840.324 kb on - strand at 4840.324 kb on - strand at 4840.969 kb on - strand at 4840.969 kb on - strand at 4840.969 kb on - strand at 4841.008 kb on - strand at 4841.008 kb on - strand at 4841.008 kb on - strand at 4841.037 kb on + strand at 4841.038 kb on - strand at 4841.038 kb on - strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.133 kb on + strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.134 kb on - strand at 4841.181 kb on + strand at 4841.182 kb on - strand at 4841.304 kb on - strand, within Pf6N2E2_4159 at 4841.304 kb on - strand, within Pf6N2E2_4159 at 4841.304 kb on - strand, within Pf6N2E2_4159
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 4,838,437 + Pf6N2E2_4156 0.52 -0.5 4,838,437 + Pf6N2E2_4156 0.52 +0.1 4,838,577 + Pf6N2E2_4156 0.59 -0.8 4,838,578 - Pf6N2E2_4156 0.59 -1.3 4,838,578 - Pf6N2E2_4156 0.59 +0.7 4,839,186 + Pf6N2E2_4156 0.88 -0.1 4,839,387 - -1.4 4,839,397 + +0.2 4,839,397 + -0.3 4,839,397 + +1.5 4,839,397 + +0.5 4,839,398 - -0.1 4,839,398 - -0.3 4,839,398 - -0.8 4,839,398 - -0.7 4,839,398 - +0.3 4,839,398 - -1.3 4,839,753 + Pf6N2E2_4157 0.37 -0.3 4,839,754 - Pf6N2E2_4157 0.37 -1.4 4,839,967 - Pf6N2E2_4157 0.61 +0.4 4,839,967 - Pf6N2E2_4157 0.61 -2.7 4,839,967 - Pf6N2E2_4157 0.61 -0.2 4,840,017 + Pf6N2E2_4157 0.67 -0.9 4,840,017 + Pf6N2E2_4157 0.67 -0.8 4,840,152 + Pf6N2E2_4157 0.82 -0.8 4,840,152 + Pf6N2E2_4157 0.82 -1.0 4,840,152 + Pf6N2E2_4157 0.82 -0.3 4,840,153 - Pf6N2E2_4157 0.82 -3.2 4,840,153 - Pf6N2E2_4157 0.82 +0.0 4,840,323 + -1.3 4,840,323 + -0.4 4,840,323 + +1.0 4,840,323 + -1.2 4,840,323 + +0.1 4,840,324 - -0.2 4,840,324 - -0.5 4,840,324 - +1.3 4,840,324 - +1.2 4,840,324 - +0.1 4,840,969 - +1.0 4,840,969 - -1.1 4,840,969 - -0.3 4,841,008 - +1.8 4,841,008 - -0.2 4,841,008 - -0.8 4,841,037 + +0.2 4,841,038 - +0.2 4,841,038 - -0.5 4,841,133 + -0.2 4,841,133 + +0.3 4,841,133 + -0.7 4,841,133 + +0.5 4,841,133 + +1.3 4,841,133 + -0.2 4,841,133 + -0.6 4,841,133 + +0.4 4,841,133 + +0.0 4,841,133 + +0.9 4,841,133 + -1.4 4,841,133 + -0.7 4,841,134 - -0.3 4,841,134 - +0.0 4,841,134 - -0.6 4,841,134 - +0.5 4,841,134 - -0.1 4,841,134 - +0.1 4,841,134 - -0.9 4,841,134 - -0.3 4,841,134 - +0.2 4,841,134 - -0.7 4,841,134 - -1.4 4,841,181 + +0.9 4,841,182 - -1.5 4,841,304 - Pf6N2E2_4159 0.23 -2.9 4,841,304 - Pf6N2E2_4159 0.23 -1.6 4,841,304 - Pf6N2E2_4159 0.23 -1.0
Or see this region's nucleotide sequence