Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2155 and Pf6N2E2_2156 are separated by 59 nucleotides Pf6N2E2_2156 and Pf6N2E2_2157 are separated by 76 nucleotides Pf6N2E2_2157 and Pf6N2E2_2158 are separated by 138 nucleotides Pf6N2E2_2158 and Pf6N2E2_2159 are separated by 33 nucleotides
Pf6N2E2_2155 - hypothetical protein, at 2,560,353 to 2,560,637
_2155
Pf6N2E2_2156 - Putative polyketide synthase (Fragment), at 2,560,697 to 2,561,365
_2156
Pf6N2E2_2157 - Transcriptional regulator, at 2,561,442 to 2,562,371
_2157
Pf6N2E2_2158 - Signal transduction histidine kinase, at 2,562,510 to 2,563,097
_2158
Pf6N2E2_2159 - phospholipase/carboxylesterase family protein, at 2,563,131 to 2,563,847
_2159
Position (kb)
2561
2562
2563 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2560.446 kb on + strand, within Pf6N2E2_2155 at 2560.586 kb on + strand, within Pf6N2E2_2155 at 2560.586 kb on + strand, within Pf6N2E2_2155 at 2560.587 kb on - strand, within Pf6N2E2_2155 at 2560.678 kb on + strand at 2560.678 kb on + strand at 2560.678 kb on + strand at 2560.678 kb on + strand at 2560.679 kb on - strand at 2560.679 kb on - strand at 2560.679 kb on - strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.682 kb on + strand at 2560.683 kb on - strand at 2560.683 kb on - strand at 2560.683 kb on - strand at 2560.683 kb on - strand at 2560.683 kb on - strand at 2560.683 kb on - strand at 2560.683 kb on - strand at 2560.683 kb on - strand at 2560.694 kb on + strand at 2560.694 kb on + strand at 2560.694 kb on + strand at 2560.694 kb on + strand at 2561.154 kb on - strand, within Pf6N2E2_2156 at 2561.159 kb on - strand, within Pf6N2E2_2156 at 2561.331 kb on + strand at 2561.362 kb on - strand at 2561.381 kb on - strand at 2561.424 kb on + strand at 2561.902 kb on + strand, within Pf6N2E2_2157 at 2561.952 kb on + strand, within Pf6N2E2_2157 at 2561.952 kb on + strand, within Pf6N2E2_2157 at 2561.953 kb on - strand, within Pf6N2E2_2157 at 2561.953 kb on - strand, within Pf6N2E2_2157 at 2561.953 kb on - strand, within Pf6N2E2_2157 at 2561.953 kb on - strand, within Pf6N2E2_2157 at 2561.953 kb on - strand, within Pf6N2E2_2157 at 2562.231 kb on + strand, within Pf6N2E2_2157 at 2562.231 kb on + strand, within Pf6N2E2_2157 at 2562.231 kb on + strand, within Pf6N2E2_2157 at 2562.232 kb on - strand, within Pf6N2E2_2157 at 2562.232 kb on - strand, within Pf6N2E2_2157 at 2562.232 kb on - strand, within Pf6N2E2_2157 at 2562.232 kb on - strand, within Pf6N2E2_2157 at 2562.259 kb on - strand, within Pf6N2E2_2157 at 2562.524 kb on + strand at 2562.525 kb on - strand at 2562.738 kb on + strand, within Pf6N2E2_2158 at 2562.738 kb on + strand, within Pf6N2E2_2158 at 2562.738 kb on + strand, within Pf6N2E2_2158 at 2562.738 kb on + strand, within Pf6N2E2_2158 at 2562.738 kb on + strand, within Pf6N2E2_2158 at 2562.739 kb on - strand, within Pf6N2E2_2158 at 2562.739 kb on - strand, within Pf6N2E2_2158 at 2562.739 kb on - strand, within Pf6N2E2_2158 at 2562.739 kb on - strand, within Pf6N2E2_2158 at 2562.739 kb on - strand, within Pf6N2E2_2158 at 2562.739 kb on - strand, within Pf6N2E2_2158 at 2563.274 kb on - strand, within Pf6N2E2_2159 at 2563.312 kb on - strand, within Pf6N2E2_2159 at 2563.312 kb on - strand, within Pf6N2E2_2159
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 2,560,446 + Pf6N2E2_2155 0.33 +1.2 2,560,586 + Pf6N2E2_2155 0.82 +0.1 2,560,586 + Pf6N2E2_2155 0.82 +1.1 2,560,587 - Pf6N2E2_2155 0.82 +0.5 2,560,678 + +0.2 2,560,678 + -0.4 2,560,678 + -0.9 2,560,678 + +0.3 2,560,679 - +0.2 2,560,679 - +0.1 2,560,679 - +0.4 2,560,682 + +1.3 2,560,682 + +0.6 2,560,682 + -0.1 2,560,682 + -0.3 2,560,682 + +0.4 2,560,682 + -0.9 2,560,682 + +0.3 2,560,682 + -0.4 2,560,682 + +0.1 2,560,683 - -1.4 2,560,683 - -0.0 2,560,683 - +0.1 2,560,683 - -0.2 2,560,683 - +0.6 2,560,683 - +0.6 2,560,683 - +2.0 2,560,683 - -0.6 2,560,694 + -2.6 2,560,694 + -1.0 2,560,694 + +1.3 2,560,694 + -0.2 2,561,154 - Pf6N2E2_2156 0.68 -0.7 2,561,159 - Pf6N2E2_2156 0.69 -0.1 2,561,331 + +0.1 2,561,362 - -0.3 2,561,381 - -0.1 2,561,424 + +0.8 2,561,902 + Pf6N2E2_2157 0.49 -0.8 2,561,952 + Pf6N2E2_2157 0.55 +1.4 2,561,952 + Pf6N2E2_2157 0.55 -0.2 2,561,953 - Pf6N2E2_2157 0.55 -0.3 2,561,953 - Pf6N2E2_2157 0.55 +0.6 2,561,953 - Pf6N2E2_2157 0.55 -1.3 2,561,953 - Pf6N2E2_2157 0.55 +0.8 2,561,953 - Pf6N2E2_2157 0.55 -0.8 2,562,231 + Pf6N2E2_2157 0.85 -1.2 2,562,231 + Pf6N2E2_2157 0.85 -1.0 2,562,231 + Pf6N2E2_2157 0.85 +1.0 2,562,232 - Pf6N2E2_2157 0.85 -0.7 2,562,232 - Pf6N2E2_2157 0.85 +0.8 2,562,232 - Pf6N2E2_2157 0.85 +0.1 2,562,232 - Pf6N2E2_2157 0.85 -0.9 2,562,259 - Pf6N2E2_2157 0.88 -0.3 2,562,524 + -0.1 2,562,525 - -0.3 2,562,738 + Pf6N2E2_2158 0.39 -0.2 2,562,738 + Pf6N2E2_2158 0.39 +0.3 2,562,738 + Pf6N2E2_2158 0.39 -1.1 2,562,738 + Pf6N2E2_2158 0.39 -0.5 2,562,738 + Pf6N2E2_2158 0.39 +1.0 2,562,739 - Pf6N2E2_2158 0.39 -0.5 2,562,739 - Pf6N2E2_2158 0.39 -0.9 2,562,739 - Pf6N2E2_2158 0.39 +0.3 2,562,739 - Pf6N2E2_2158 0.39 +0.3 2,562,739 - Pf6N2E2_2158 0.39 -2.6 2,562,739 - Pf6N2E2_2158 0.39 +1.4 2,563,274 - Pf6N2E2_2159 0.20 -0.2 2,563,312 - Pf6N2E2_2159 0.25 +0.2 2,563,312 - Pf6N2E2_2159 0.25 +1.2
Or see this region's nucleotide sequence