Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1799 and Pf6N2E2_1800 are separated by 10 nucleotides Pf6N2E2_1800 and Pf6N2E2_1801 are separated by 39 nucleotides Pf6N2E2_1801 and Pf6N2E2_1802 are separated by 150 nucleotides Pf6N2E2_1802 and Pf6N2E2_1803 overlap by 8 nucleotides
Pf6N2E2_1799 - ABC transporter permease protein, at 2,129,047 to 2,129,925
_1799
Pf6N2E2_1800 - putative ABC transporter ATP-binding protein, at 2,129,936 to 2,130,700
_1800
Pf6N2E2_1801 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 2,130,740 to 2,131,564
_1801
Pf6N2E2_1802 - polysaccharide deacetylase, at 2,131,715 to 2,132,563
_1802
Pf6N2E2_1803 - Polysaccharide deacetylase, at 2,132,556 to 2,133,443
_1803
Position (kb)
2130
2131
2132 Strain fitness (log2 ratio)
-1
0
1
2 at 2129.774 kb on - strand, within Pf6N2E2_1799 at 2129.774 kb on - strand, within Pf6N2E2_1799 at 2129.791 kb on + strand, within Pf6N2E2_1799 at 2129.792 kb on - strand, within Pf6N2E2_1799 at 2129.972 kb on + strand at 2129.972 kb on + strand at 2129.973 kb on - strand at 2129.973 kb on - strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.698 kb on + strand at 2130.699 kb on - strand at 2130.917 kb on + strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2131.083 kb on - strand, within Pf6N2E2_1801 at 2131.106 kb on + strand, within Pf6N2E2_1801 at 2131.107 kb on - strand, within Pf6N2E2_1801 at 2131.107 kb on - strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.678 kb on + strand at 2131.678 kb on + strand at 2131.679 kb on - strand at 2131.687 kb on + strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.751 kb on + strand at 2131.751 kb on + strand at 2131.752 kb on - strand at 2131.838 kb on + strand, within Pf6N2E2_1802 at 2131.945 kb on - strand, within Pf6N2E2_1802 at 2132.176 kb on + strand, within Pf6N2E2_1802 at 2132.177 kb on - strand, within Pf6N2E2_1802 at 2132.181 kb on - strand, within Pf6N2E2_1802 at 2132.214 kb on - strand, within Pf6N2E2_1802 at 2132.282 kb on + strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.403 kb on + strand, within Pf6N2E2_1802 at 2132.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 2,129,774 - Pf6N2E2_1799 0.83 -0.7 2,129,774 - Pf6N2E2_1799 0.83 -0.2 2,129,791 + Pf6N2E2_1799 0.85 -0.3 2,129,792 - Pf6N2E2_1799 0.85 +0.0 2,129,972 + +1.4 2,129,972 + -0.5 2,129,973 - -0.9 2,129,973 - -0.6 2,130,648 + +0.4 2,130,648 + +2.1 2,130,648 + -0.4 2,130,648 + +0.2 2,130,648 + +0.2 2,130,698 + +0.2 2,130,699 - -0.4 2,130,917 + Pf6N2E2_1801 0.21 +0.3 2,130,918 - Pf6N2E2_1801 0.22 +0.6 2,130,918 - Pf6N2E2_1801 0.22 -0.9 2,130,918 - Pf6N2E2_1801 0.22 +0.1 2,131,083 - Pf6N2E2_1801 0.42 -1.7 2,131,106 + Pf6N2E2_1801 0.44 +0.4 2,131,107 - Pf6N2E2_1801 0.44 -0.2 2,131,107 - Pf6N2E2_1801 0.44 -1.0 2,131,370 + Pf6N2E2_1801 0.76 +1.2 2,131,370 + Pf6N2E2_1801 0.76 -0.6 2,131,370 + Pf6N2E2_1801 0.76 -0.3 2,131,370 + Pf6N2E2_1801 0.76 -1.0 2,131,370 + Pf6N2E2_1801 0.76 -0.4 2,131,370 + Pf6N2E2_1801 0.76 +0.1 2,131,371 - Pf6N2E2_1801 0.76 -0.7 2,131,371 - Pf6N2E2_1801 0.76 -1.0 2,131,371 - Pf6N2E2_1801 0.76 -0.8 2,131,371 - Pf6N2E2_1801 0.76 +0.7 2,131,678 + +0.0 2,131,678 + +0.3 2,131,679 - -0.4 2,131,687 + +0.6 2,131,688 - +0.2 2,131,688 - -0.0 2,131,688 - -0.1 2,131,751 + +1.9 2,131,751 + +0.8 2,131,752 - -0.0 2,131,838 + Pf6N2E2_1802 0.14 -0.6 2,131,945 - Pf6N2E2_1802 0.27 +1.4 2,132,176 + Pf6N2E2_1802 0.54 -1.3 2,132,177 - Pf6N2E2_1802 0.54 -0.4 2,132,181 - Pf6N2E2_1802 0.55 -0.0 2,132,214 - Pf6N2E2_1802 0.59 -0.2 2,132,282 + Pf6N2E2_1802 0.67 +1.0 2,132,298 - Pf6N2E2_1802 0.69 +0.3 2,132,298 - Pf6N2E2_1802 0.69 +0.6 2,132,403 + Pf6N2E2_1802 0.81 +0.2 2,132,502 + +0.3
Or see this region's nucleotide sequence