Experiment: H2 N-free with 10mM D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00380 and MMP_RS00385 are separated by 201 nucleotides MMP_RS00385 and MMP_RS00390 are separated by 28 nucleotides MMP_RS00390 and MMP_RS00395 are separated by 60 nucleotides MMP_RS00395 and MMP_RS00400 are separated by 253 nucleotides
MMP_RS00380: MMP_RS00380 - P-II family nitrogen regulator, at 78,446 to 78,784
_RS00380
MMP_RS00385: MMP_RS00385 - P-II family nitrogen regulator, at 78,986 to 79,324
_RS00385
MMP_RS00390: MMP_RS00390 - ammonium transporter, at 79,353 to 80,588
_RS00390
MMP_RS00395: MMP_RS00395 - MGMT family protein, at 80,649 to 80,948
_RS00395
MMP_RS00400: MMP_RS00400 - tributyrin esterase, at 81,202 to 82,140
_RS00400
Position (kb) 79
80
81 Strain fitness (log2 ratio) -2
-1
0
1 at 78.372 kb on + strand at 78.375 kb on - strand at 78.376 kb on + strand at 78.378 kb on - strand at 78.378 kb on - strand at 78.378 kb on - strand at 78.379 kb on + strand at 78.381 kb on - strand at 78.381 kb on - strand at 78.381 kb on - strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.384 kb on + strand at 78.617 kb on + strand, within MMP_RS00380 at 78.641 kb on + strand, within MMP_RS00380 at 78.663 kb on - strand, within MMP_RS00380 at 78.723 kb on - strand, within MMP_RS00380 at 78.745 kb on - strand, within MMP_RS00380 at 78.746 kb on + strand, within MMP_RS00380 at 78.782 kb on - strand at 78.783 kb on + strand at 78.833 kb on - strand at 78.847 kb on - strand at 78.847 kb on - strand at 78.848 kb on + strand at 78.848 kb on + strand at 78.972 kb on - strand at 79.213 kb on + strand, within MMP_RS00385 at 79.229 kb on - strand, within MMP_RS00385 at 79.231 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.323 kb on + strand at 79.323 kb on + strand at 79.325 kb on - strand at 79.325 kb on - strand at 79.348 kb on - strand at 79.348 kb on - strand at 79.466 kb on + strand at 79.607 kb on + strand, within MMP_RS00390 at 79.919 kb on + strand, within MMP_RS00390 at 80.013 kb on - strand, within MMP_RS00390 at 80.013 kb on - strand, within MMP_RS00390 at 80.014 kb on + strand, within MMP_RS00390 at 80.072 kb on - strand, within MMP_RS00390 at 80.086 kb on - strand, within MMP_RS00390 at 80.158 kb on - strand, within MMP_RS00390 at 80.158 kb on - strand, within MMP_RS00390 at 80.158 kb on - strand, within MMP_RS00390 at 80.161 kb on + strand, within MMP_RS00390 at 80.161 kb on + strand, within MMP_RS00390 at 80.161 kb on + strand, within MMP_RS00390 at 80.240 kb on + strand, within MMP_RS00390 at 80.279 kb on - strand, within MMP_RS00390 at 80.311 kb on - strand, within MMP_RS00390 at 80.312 kb on + strand, within MMP_RS00390 at 80.641 kb on - strand at 80.819 kb on + strand, within MMP_RS00395 at 80.952 kb on - strand at 81.070 kb on - strand at 81.075 kb on - strand at 81.086 kb on - strand at 81.087 kb on + strand at 81.089 kb on + strand at 81.122 kb on - strand at 81.161 kb on + strand at 81.164 kb on - strand at 81.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-Alanine remove 78,372 + +0.5 78,375 - +0.3 78,376 + +0.5 78,378 - +1.5 78,378 - +1.1 78,378 - +1.4 78,379 + +0.9 78,381 - +0.6 78,381 - +0.9 78,381 - +0.9 78,382 + -0.8 78,382 + +0.7 78,382 + +0.6 78,382 + -0.5 78,384 + +1.3 78,617 + MMP_RS00380 0.50 -0.3 78,641 + MMP_RS00380 0.58 +0.3 78,663 - MMP_RS00380 0.64 +1.7 78,723 - MMP_RS00380 0.82 +1.0 78,745 - MMP_RS00380 0.88 +1.2 78,746 + MMP_RS00380 0.88 +0.5 78,782 - +0.5 78,783 + +0.2 78,833 - +1.0 78,847 - +0.1 78,847 - +0.4 78,848 + -0.0 78,848 + +0.6 78,972 - +0.8 79,213 + MMP_RS00385 0.67 -0.4 79,229 - MMP_RS00385 0.72 +0.8 79,231 - MMP_RS00385 0.72 +0.7 79,281 - MMP_RS00385 0.87 +0.2 79,281 - MMP_RS00385 0.87 +0.3 79,281 - MMP_RS00385 0.87 +0.1 79,323 + +1.3 79,323 + -0.1 79,325 - -0.1 79,325 - +0.5 79,348 - -0.0 79,348 - -0.0 79,466 + +0.0 79,607 + MMP_RS00390 0.21 +0.6 79,919 + MMP_RS00390 0.46 +0.5 80,013 - MMP_RS00390 0.53 +0.3 80,013 - MMP_RS00390 0.53 +0.2 80,014 + MMP_RS00390 0.53 +0.9 80,072 - MMP_RS00390 0.58 +0.6 80,086 - MMP_RS00390 0.59 -1.8 80,158 - MMP_RS00390 0.65 +0.2 80,158 - MMP_RS00390 0.65 -1.0 80,158 - MMP_RS00390 0.65 -0.6 80,161 + MMP_RS00390 0.65 -0.2 80,161 + MMP_RS00390 0.65 +0.7 80,161 + MMP_RS00390 0.65 +1.1 80,240 + MMP_RS00390 0.72 +0.9 80,279 - MMP_RS00390 0.75 -1.0 80,311 - MMP_RS00390 0.78 +0.7 80,312 + MMP_RS00390 0.78 +0.9 80,641 - -0.1 80,819 + MMP_RS00395 0.57 +1.0 80,952 - +1.4 81,070 - +0.0 81,075 - +0.0 81,086 - +0.1 81,087 + +1.5 81,089 + +0.9 81,122 - -0.0 81,161 + -0.2 81,164 - +0.8 81,181 + +0.5
Or see this region's nucleotide sequence