Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_479

Experiment: Vanillyl Alcohol 0.005 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotidesPf6N2E2_479 and Pf6N2E2_480 are separated by 0 nucleotides Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data), at 543,500 to 544,885 _478 Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data), at 544,889 to 546,160 _479 Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data), at 546,161 to 547,219 _480 Position (kb) 544 545 546 547Strain fitness (log2 ratio) -2 -1 0 1at 544.195 kb on - strand, within Pf6N2E2_478at 544.195 kb on - strand, within Pf6N2E2_478at 544.341 kb on + strand, within Pf6N2E2_478at 544.342 kb on - strand, within Pf6N2E2_478at 544.830 kb on + strandat 544.891 kb on - strandat 545.075 kb on + strand, within Pf6N2E2_479at 545.497 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.643 kb on + strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479at 546.053 kb on + strandat 546.053 kb on + strandat 546.146 kb on - strandat 546.216 kb on + strandat 546.216 kb on + strandat 546.217 kb on - strandat 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.483 kb on + strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.484 kb on - strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.498 kb on + strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strand, within Pf6N2E2_480at 546.499 kb on - strandat 546.561 kb on + strand, within Pf6N2E2_480at 546.561 kb on + strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.562 kb on - strand, within Pf6N2E2_480at 546.565 kb on - strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.645 kb on + strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.646 kb on - strand, within Pf6N2E2_480at 546.721 kb on - strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.804 kb on + strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.805 kb on - strand, within Pf6N2E2_480at 546.913 kb on - strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.918 kb on + strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 546.919 kb on - strand, within Pf6N2E2_480at 547.032 kb on + strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.033 kb on - strand, within Pf6N2E2_480at 547.077 kb on + strand, within Pf6N2E2_480at 547.078 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vanillyl Alcohol 0.005 M
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544,195 - Pf6N2E2_478 0.50 +0.4
544,195 - Pf6N2E2_478 0.50 -0.3
544,341 + Pf6N2E2_478 0.61 -0.4
544,342 - Pf6N2E2_478 0.61 +0.1
544,830 + +0.1
544,891 - -0.8
545,075 + Pf6N2E2_479 0.15 -1.3
545,497 - Pf6N2E2_479 0.48 -1.0
545,599 - Pf6N2E2_479 0.56 -0.6
545,599 - Pf6N2E2_479 0.56 -1.6
545,643 + Pf6N2E2_479 0.59 +0.4
545,644 - Pf6N2E2_479 0.59 -0.3
545,644 - Pf6N2E2_479 0.59 -2.4
546,053 + -0.8
546,053 + +0.1
546,146 - -1.0
546,216 + +0.2
546,216 + -0.4
546,217 - -0.4
546,483 + Pf6N2E2_480 0.30 +0.1
546,483 + Pf6N2E2_480 0.30 -1.9
546,483 + Pf6N2E2_480 0.30 +1.4
546,483 + Pf6N2E2_480 0.30 +0.6
546,483 + Pf6N2E2_480 0.30 -0.4
546,484 - Pf6N2E2_480 0.31 +0.3
546,484 - Pf6N2E2_480 0.31 -0.2
546,484 - Pf6N2E2_480 0.31 -0.5
546,484 - Pf6N2E2_480 0.31 +0.2
546,484 - Pf6N2E2_480 0.31 -1.4
546,484 - Pf6N2E2_480 0.31 +0.4
546,484 - Pf6N2E2_480 0.31 -0.6
546,484 - Pf6N2E2_480 0.31 -0.9
546,484 - Pf6N2E2_480 0.31 -0.5
546,484 - Pf6N2E2_480 0.31 -0.5
546,484 - Pf6N2E2_480 0.31 -1.2
546,498 + Pf6N2E2_480 0.32 -1.2
546,498 + Pf6N2E2_480 0.32 -1.1
546,498 + Pf6N2E2_480 0.32 -0.4
546,499 - Pf6N2E2_480 0.32 -1.2
546,499 - Pf6N2E2_480 0.32 +0.4
546,499 - -0.2
546,561 + Pf6N2E2_480 0.38 -0.7
546,561 + Pf6N2E2_480 0.38 +0.4
546,562 - Pf6N2E2_480 0.38 +0.7
546,562 - Pf6N2E2_480 0.38 -1.5
546,562 - Pf6N2E2_480 0.38 +0.9
546,565 - Pf6N2E2_480 0.38 -0.7
546,645 + Pf6N2E2_480 0.46 +0.1
546,645 + Pf6N2E2_480 0.46 +0.2
546,645 + Pf6N2E2_480 0.46 -1.1
546,646 - Pf6N2E2_480 0.46 -0.4
546,646 - Pf6N2E2_480 0.46 -0.7
546,721 - Pf6N2E2_480 0.53 +1.6
546,804 + Pf6N2E2_480 0.61 -0.8
546,804 + Pf6N2E2_480 0.61 +0.5
546,804 + Pf6N2E2_480 0.61 -1.2
546,804 + Pf6N2E2_480 0.61 -2.7
546,804 + Pf6N2E2_480 0.61 +1.6
546,805 - Pf6N2E2_480 0.61 +0.3
546,805 - Pf6N2E2_480 0.61 -0.2
546,913 - Pf6N2E2_480 0.71 -0.3
546,918 + Pf6N2E2_480 0.71 -0.3
546,918 + Pf6N2E2_480 0.71 +0.4
546,918 + Pf6N2E2_480 0.71 +0.2
546,918 + Pf6N2E2_480 0.71 -0.4
546,919 - Pf6N2E2_480 0.72 +0.6
546,919 - Pf6N2E2_480 0.72 +0.7
546,919 - Pf6N2E2_480 0.72 -0.4
546,919 - Pf6N2E2_480 0.72 +0.2
547,032 + Pf6N2E2_480 0.82 +0.7
547,033 - Pf6N2E2_480 0.82 +1.6
547,033 - Pf6N2E2_480 0.82 -0.0
547,033 - Pf6N2E2_480 0.82 +0.1
547,077 + Pf6N2E2_480 0.86 -0.2
547,078 - -1.2

Or see this region's nucleotide sequence