Experiment: H2 N-free with 10mM D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00365 and MMP_RS00370 are separated by 391 nucleotides MMP_RS00370 and MMP_RS00375 are separated by 26 nucleotides MMP_RS00375 and MMP_RS00380 are separated by 103 nucleotides MMP_RS00380 and MMP_RS00385 are separated by 201 nucleotides
MMP_RS00365: MMP_RS00365 - acetylglutamate kinase, at 75,475 to 76,359
_RS00365
MMP_RS00370: MMP_RS00370 - P-II family nitrogen regulator, at 76,751 to 77,089
_RS00370
MMP_RS00375: MMP_RS00375 - ammonium transporter, at 77,116 to 78,342
_RS00375
MMP_RS00380: MMP_RS00380 - P-II family nitrogen regulator, at 78,446 to 78,784
_RS00380
MMP_RS00385: MMP_RS00385 - P-II family nitrogen regulator, at 78,986 to 79,324
_RS00385
Position (kb)
77
78
79 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 76.350 kb on + strand at 76.402 kb on + strand at 76.413 kb on + strand at 76.418 kb on - strand at 76.469 kb on - strand at 76.470 kb on + strand at 76.475 kb on - strand at 76.475 kb on - strand at 76.479 kb on - strand at 76.496 kb on - strand at 76.497 kb on + strand at 76.497 kb on + strand at 76.551 kb on - strand at 76.552 kb on + strand at 76.571 kb on + strand at 76.634 kb on - strand at 76.635 kb on + strand at 76.636 kb on - strand at 76.636 kb on - strand at 76.636 kb on - strand at 76.637 kb on + strand at 76.768 kb on - strand at 77.037 kb on - strand at 77.076 kb on - strand at 77.193 kb on + strand at 77.201 kb on - strand at 77.204 kb on - strand at 77.205 kb on + strand at 77.292 kb on + strand, within MMP_RS00375 at 77.405 kb on - strand, within MMP_RS00375 at 77.535 kb on + strand, within MMP_RS00375 at 77.565 kb on + strand, within MMP_RS00375 at 77.695 kb on + strand, within MMP_RS00375 at 77.829 kb on - strand, within MMP_RS00375 at 77.830 kb on + strand, within MMP_RS00375 at 77.830 kb on + strand, within MMP_RS00375 at 77.841 kb on + strand, within MMP_RS00375 at 77.844 kb on + strand, within MMP_RS00375 at 77.912 kb on - strand, within MMP_RS00375 at 77.913 kb on + strand, within MMP_RS00375 at 77.945 kb on - strand, within MMP_RS00375 at 77.975 kb on - strand, within MMP_RS00375 at 77.984 kb on - strand, within MMP_RS00375 at 78.068 kb on - strand, within MMP_RS00375 at 78.165 kb on + strand, within MMP_RS00375 at 78.174 kb on + strand, within MMP_RS00375 at 78.273 kb on - strand at 78.372 kb on + strand at 78.375 kb on - strand at 78.376 kb on + strand at 78.378 kb on - strand at 78.378 kb on - strand at 78.378 kb on - strand at 78.379 kb on + strand at 78.381 kb on - strand at 78.381 kb on - strand at 78.381 kb on - strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.382 kb on + strand at 78.384 kb on + strand at 78.617 kb on + strand, within MMP_RS00380 at 78.641 kb on + strand, within MMP_RS00380 at 78.663 kb on - strand, within MMP_RS00380 at 78.723 kb on - strand, within MMP_RS00380 at 78.745 kb on - strand, within MMP_RS00380 at 78.746 kb on + strand, within MMP_RS00380 at 78.782 kb on - strand at 78.783 kb on + strand at 78.833 kb on - strand at 78.847 kb on - strand at 78.847 kb on - strand at 78.848 kb on + strand at 78.848 kb on + strand at 78.972 kb on - strand at 79.213 kb on + strand, within MMP_RS00385 at 79.229 kb on - strand, within MMP_RS00385 at 79.231 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.281 kb on - strand, within MMP_RS00385 at 79.323 kb on + strand at 79.323 kb on + strand at 79.325 kb on - strand at 79.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-Alanine remove 76,350 + -2.0 76,402 + -1.1 76,413 + +0.4 76,418 - +0.0 76,469 - -0.7 76,470 + -0.3 76,475 - -0.3 76,475 - +0.7 76,479 - +0.1 76,496 - -0.6 76,497 + -2.3 76,497 + +0.1 76,551 - +0.0 76,552 + +0.7 76,571 + +0.0 76,634 - -0.8 76,635 + -1.2 76,636 - +1.4 76,636 - -0.9 76,636 - -1.5 76,637 + -0.7 76,768 - -2.2 77,037 - -2.5 77,076 - -3.5 77,193 + -2.6 77,201 - -2.2 77,204 - -1.2 77,205 + -1.6 77,292 + MMP_RS00375 0.14 -2.6 77,405 - MMP_RS00375 0.24 -1.4 77,535 + MMP_RS00375 0.34 -2.2 77,565 + MMP_RS00375 0.37 -1.0 77,695 + MMP_RS00375 0.47 -2.4 77,829 - MMP_RS00375 0.58 -1.0 77,830 + MMP_RS00375 0.58 -1.1 77,830 + MMP_RS00375 0.58 -1.1 77,841 + MMP_RS00375 0.59 -1.4 77,844 + MMP_RS00375 0.59 -3.9 77,912 - MMP_RS00375 0.65 -2.2 77,913 + MMP_RS00375 0.65 -1.7 77,945 - MMP_RS00375 0.68 -1.1 77,975 - MMP_RS00375 0.70 -0.9 77,984 - MMP_RS00375 0.71 -0.8 78,068 - MMP_RS00375 0.78 -2.8 78,165 + MMP_RS00375 0.85 -2.0 78,174 + MMP_RS00375 0.86 -5.5 78,273 - -2.6 78,372 + -0.1 78,375 - -0.1 78,376 + +0.1 78,378 - +1.0 78,378 - +1.1 78,378 - +1.4 78,379 + +0.6 78,381 - +0.6 78,381 - -0.5 78,381 - +0.7 78,382 + -1.1 78,382 + -0.1 78,382 + +0.9 78,382 + -1.6 78,384 + +0.9 78,617 + MMP_RS00380 0.50 +0.3 78,641 + MMP_RS00380 0.58 +1.0 78,663 - MMP_RS00380 0.64 +1.3 78,723 - MMP_RS00380 0.82 +0.9 78,745 - MMP_RS00380 0.88 +0.8 78,746 + MMP_RS00380 0.88 -0.1 78,782 - +0.3 78,783 + -0.4 78,833 - +1.0 78,847 - +0.2 78,847 - +1.7 78,848 + -0.2 78,848 + +1.3 78,972 - +1.3 79,213 + MMP_RS00385 0.67 +0.5 79,229 - MMP_RS00385 0.72 +0.5 79,231 - MMP_RS00385 0.72 +0.7 79,281 - MMP_RS00385 0.87 +0.2 79,281 - MMP_RS00385 0.87 +0.4 79,281 - MMP_RS00385 0.87 +1.2 79,323 + +0.9 79,323 + +1.0 79,325 - -0.5 79,325 - -0.0
Or see this region's nucleotide sequence