Experiment: Survival; Treatment=FW300 (pH7.4) with 0.2 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04085 and IAI47_04090 are separated by 10 nucleotides IAI47_04090 and IAI47_04095 are separated by 45 nucleotides IAI47_04095 and IAI47_04100 are separated by 19 nucleotides
IAI47_04085: IAI47_04085 - glucarate dehydratase, at 853,953 to 855,299
_04085
IAI47_04090: IAI47_04090 - glucarate dehydratase, at 855,310 to 856,641
_04090
IAI47_04095: IAI47_04095 - 2-dehydro-3-deoxyglucarate aldolase, at 856,687 to 857,457
_04095
IAI47_04100: IAI47_04100 - 2-hydroxy-3-oxopropionate reductase, at 857,477 to 858,361
_04100
Position (kb) 855
856
857 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 854.338 kb on + strand, within IAI47_04085 at 854.339 kb on - strand, within IAI47_04085 at 854.398 kb on - strand, within IAI47_04085 at 854.398 kb on - strand, within IAI47_04085 at 854.398 kb on - strand, within IAI47_04085 at 854.406 kb on + strand, within IAI47_04085 at 854.406 kb on + strand, within IAI47_04085 at 854.406 kb on + strand, within IAI47_04085 at 854.406 kb on + strand, within IAI47_04085 at 854.406 kb on + strand, within IAI47_04085 at 854.406 kb on + strand, within IAI47_04085 at 854.407 kb on - strand, within IAI47_04085 at 854.407 kb on - strand, within IAI47_04085 at 854.407 kb on - strand, within IAI47_04085 at 854.423 kb on + strand, within IAI47_04085 at 854.423 kb on + strand, within IAI47_04085 at 854.440 kb on - strand, within IAI47_04085 at 854.444 kb on + strand, within IAI47_04085 at 854.444 kb on + strand, within IAI47_04085 at 854.445 kb on - strand, within IAI47_04085 at 854.445 kb on - strand, within IAI47_04085 at 854.472 kb on + strand, within IAI47_04085 at 854.472 kb on + strand, within IAI47_04085 at 854.472 kb on + strand, within IAI47_04085 at 854.472 kb on + strand, within IAI47_04085 at 854.472 kb on + strand, within IAI47_04085 at 854.473 kb on - strand, within IAI47_04085 at 854.473 kb on - strand, within IAI47_04085 at 854.473 kb on - strand, within IAI47_04085 at 854.473 kb on - strand, within IAI47_04085 at 854.540 kb on + strand, within IAI47_04085 at 854.540 kb on + strand, within IAI47_04085 at 854.540 kb on + strand, within IAI47_04085 at 854.540 kb on + strand, within IAI47_04085 at 854.540 kb on + strand, within IAI47_04085 at 854.541 kb on - strand, within IAI47_04085 at 854.541 kb on - strand, within IAI47_04085 at 854.544 kb on + strand, within IAI47_04085 at 854.544 kb on + strand, within IAI47_04085 at 854.545 kb on - strand, within IAI47_04085 at 854.552 kb on + strand, within IAI47_04085 at 854.553 kb on - strand, within IAI47_04085 at 854.643 kb on - strand, within IAI47_04085 at 854.643 kb on - strand, within IAI47_04085 at 854.657 kb on + strand, within IAI47_04085 at 854.657 kb on + strand, within IAI47_04085 at 854.658 kb on - strand, within IAI47_04085 at 854.696 kb on + strand, within IAI47_04085 at 854.696 kb on + strand, within IAI47_04085 at 854.696 kb on + strand, within IAI47_04085 at 854.697 kb on - strand, within IAI47_04085 at 854.697 kb on - strand, within IAI47_04085 at 854.721 kb on + strand, within IAI47_04085 at 854.721 kb on + strand, within IAI47_04085 at 854.721 kb on + strand, within IAI47_04085 at 854.722 kb on - strand, within IAI47_04085 at 854.754 kb on + strand, within IAI47_04085 at 854.755 kb on - strand, within IAI47_04085 at 854.755 kb on - strand, within IAI47_04085 at 854.755 kb on - strand, within IAI47_04085 at 854.816 kb on + strand, within IAI47_04085 at 854.816 kb on + strand, within IAI47_04085 at 854.816 kb on + strand, within IAI47_04085 at 854.816 kb on + strand, within IAI47_04085 at 854.816 kb on + strand, within IAI47_04085 at 854.817 kb on - strand, within IAI47_04085 at 854.817 kb on - strand, within IAI47_04085 at 854.817 kb on - strand, within IAI47_04085 at 854.817 kb on - strand, within IAI47_04085 at 854.972 kb on + strand, within IAI47_04085 at 854.973 kb on - strand, within IAI47_04085 at 854.984 kb on + strand, within IAI47_04085 at 854.985 kb on - strand, within IAI47_04085 at 854.985 kb on - strand, within IAI47_04085 at 855.002 kb on + strand, within IAI47_04085 at 855.003 kb on - strand, within IAI47_04085 at 855.047 kb on + strand, within IAI47_04085 at 855.169 kb on + strand at 855.169 kb on + strand at 855.169 kb on + strand at 855.170 kb on - strand at 855.170 kb on - strand at 855.170 kb on - strand at 855.189 kb on + strand at 855.189 kb on + strand at 855.190 kb on - strand at 855.190 kb on - strand at 855.237 kb on + strand at 855.237 kb on + strand at 855.237 kb on + strand at 855.237 kb on + strand at 855.237 kb on + strand at 855.237 kb on + strand at 855.237 kb on + strand at 855.237 kb on + strand at 855.238 kb on - strand at 855.238 kb on - strand at 855.238 kb on - strand at 855.238 kb on - strand at 855.238 kb on - strand at 855.238 kb on - strand at 855.297 kb on + strand at 855.297 kb on + strand at 855.297 kb on + strand at 855.298 kb on - strand at 855.298 kb on - strand at 855.298 kb on - strand at 855.298 kb on - strand at 855.298 kb on - strand at 855.345 kb on + strand at 855.346 kb on - strand at 855.346 kb on - strand at 855.361 kb on + strand at 855.362 kb on - strand at 855.362 kb on - strand at 855.435 kb on + strand at 855.748 kb on + strand, within IAI47_04090 at 855.749 kb on - strand, within IAI47_04090 at 855.757 kb on + strand, within IAI47_04090 at 855.757 kb on + strand, within IAI47_04090 at 855.758 kb on - strand, within IAI47_04090 at 855.758 kb on - strand, within IAI47_04090 at 855.758 kb on - strand, within IAI47_04090 at 855.758 kb on - strand, within IAI47_04090 at 855.758 kb on - strand, within IAI47_04090 at 855.775 kb on - strand, within IAI47_04090 at 855.791 kb on - strand, within IAI47_04090 at 855.823 kb on + strand, within IAI47_04090 at 855.823 kb on + strand, within IAI47_04090 at 855.839 kb on + strand, within IAI47_04090 at 855.866 kb on + strand, within IAI47_04090 at 855.867 kb on - strand, within IAI47_04090 at 855.867 kb on - strand, within IAI47_04090 at 855.887 kb on - strand, within IAI47_04090 at 855.887 kb on - strand, within IAI47_04090 at 855.895 kb on + strand, within IAI47_04090 at 855.895 kb on + strand, within IAI47_04090 at 855.896 kb on - strand, within IAI47_04090 at 855.896 kb on - strand, within IAI47_04090 at 855.908 kb on - strand, within IAI47_04090 at 855.993 kb on + strand, within IAI47_04090 at 856.056 kb on + strand, within IAI47_04090 at 856.056 kb on + strand, within IAI47_04090 at 856.056 kb on + strand, within IAI47_04090 at 856.056 kb on + strand, within IAI47_04090 at 856.056 kb on + strand, within IAI47_04090 at 856.057 kb on - strand, within IAI47_04090 at 856.057 kb on - strand, within IAI47_04090 at 856.057 kb on - strand, within IAI47_04090 at 856.060 kb on + strand, within IAI47_04090 at 856.073 kb on - strand, within IAI47_04090 at 856.105 kb on + strand, within IAI47_04090 at 856.105 kb on + strand, within IAI47_04090 at 856.106 kb on - strand, within IAI47_04090 at 856.167 kb on + strand, within IAI47_04090 at 856.167 kb on + strand, within IAI47_04090 at 856.167 kb on + strand, within IAI47_04090 at 856.167 kb on + strand, within IAI47_04090 at 856.168 kb on - strand, within IAI47_04090 at 856.168 kb on - strand, within IAI47_04090 at 856.168 kb on - strand, within IAI47_04090 at 856.200 kb on + strand, within IAI47_04090 at 856.201 kb on - strand, within IAI47_04090 at 856.354 kb on - strand, within IAI47_04090 at 856.354 kb on - strand, within IAI47_04090 at 856.398 kb on + strand, within IAI47_04090 at 856.399 kb on - strand, within IAI47_04090 at 856.399 kb on - strand, within IAI47_04090 at 856.455 kb on + strand, within IAI47_04090 at 856.455 kb on + strand, within IAI47_04090 at 856.464 kb on + strand, within IAI47_04090 at 856.464 kb on + strand, within IAI47_04090 at 856.499 kb on + strand, within IAI47_04090 at 856.510 kb on - strand at 856.541 kb on - strand at 856.592 kb on + strand at 856.617 kb on + strand at 856.618 kb on - strand at 856.692 kb on + strand at 856.692 kb on + strand at 856.692 kb on + strand at 856.692 kb on + strand at 856.692 kb on + strand at 856.693 kb on - strand at 856.693 kb on - strand at 856.862 kb on + strand, within IAI47_04095 at 856.863 kb on - strand, within IAI47_04095 at 856.938 kb on + strand, within IAI47_04095 at 856.938 kb on + strand, within IAI47_04095 at 856.938 kb on + strand, within IAI47_04095 at 856.939 kb on - strand, within IAI47_04095 at 856.939 kb on - strand, within IAI47_04095 at 856.939 kb on - strand, within IAI47_04095 at 856.963 kb on - strand, within IAI47_04095 at 857.080 kb on + strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.081 kb on - strand, within IAI47_04095 at 857.098 kb on + strand, within IAI47_04095 at 857.098 kb on + strand, within IAI47_04095 at 857.098 kb on + strand, within IAI47_04095 at 857.099 kb on - strand, within IAI47_04095 at 857.243 kb on - strand, within IAI47_04095 at 857.283 kb on + strand, within IAI47_04095 at 857.284 kb on - strand, within IAI47_04095 at 857.342 kb on + strand, within IAI47_04095 at 857.362 kb on + strand, within IAI47_04095 at 857.435 kb on + strand at 857.449 kb on - strand at 857.449 kb on - strand at 857.449 kb on - strand at 857.463 kb on + strand at 857.464 kb on - strand at 857.491 kb on + strand at 857.556 kb on + strand at 857.557 kb on - strand at 857.557 kb on - strand at 857.596 kb on + strand, within IAI47_04100 at 857.597 kb on - strand, within IAI47_04100
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 0.2 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 854,338 + IAI47_04085 0.29 +1.9 854,339 - IAI47_04085 0.29 -1.3 854,398 - IAI47_04085 0.33 -2.2 854,398 - IAI47_04085 0.33 +0.4 854,398 - IAI47_04085 0.33 +0.3 854,406 + IAI47_04085 0.34 -1.2 854,406 + IAI47_04085 0.34 +0.8 854,406 + IAI47_04085 0.34 -0.0 854,406 + IAI47_04085 0.34 -1.0 854,406 + IAI47_04085 0.34 +0.3 854,406 + IAI47_04085 0.34 -0.3 854,407 - IAI47_04085 0.34 -0.9 854,407 - IAI47_04085 0.34 +0.5 854,407 - IAI47_04085 0.34 -1.0 854,423 + IAI47_04085 0.35 -0.4 854,423 + IAI47_04085 0.35 +0.3 854,440 - IAI47_04085 0.36 -1.3 854,444 + IAI47_04085 0.36 +0.8 854,444 + IAI47_04085 0.36 -1.2 854,445 - IAI47_04085 0.37 +1.5 854,445 - IAI47_04085 0.37 -0.0 854,472 + IAI47_04085 0.39 +0.4 854,472 + IAI47_04085 0.39 +0.7 854,472 + IAI47_04085 0.39 +1.0 854,472 + IAI47_04085 0.39 +0.2 854,472 + IAI47_04085 0.39 +1.4 854,473 - IAI47_04085 0.39 -0.0 854,473 - IAI47_04085 0.39 -0.4 854,473 - IAI47_04085 0.39 +0.3 854,473 - IAI47_04085 0.39 -0.4 854,540 + IAI47_04085 0.44 +1.9 854,540 + IAI47_04085 0.44 +0.7 854,540 + IAI47_04085 0.44 +0.9 854,540 + IAI47_04085 0.44 +0.4 854,540 + IAI47_04085 0.44 -2.9 854,541 - IAI47_04085 0.44 -1.5 854,541 - IAI47_04085 0.44 -0.7 854,544 + IAI47_04085 0.44 -0.1 854,544 + IAI47_04085 0.44 -1.2 854,545 - IAI47_04085 0.44 +0.1 854,552 + IAI47_04085 0.44 +1.3 854,553 - IAI47_04085 0.45 -0.7 854,643 - IAI47_04085 0.51 +1.3 854,643 - IAI47_04085 0.51 -0.2 854,657 + IAI47_04085 0.52 -1.0 854,657 + IAI47_04085 0.52 -0.8 854,658 - IAI47_04085 0.52 -1.3 854,696 + IAI47_04085 0.55 -0.9 854,696 + IAI47_04085 0.55 -0.6 854,696 + IAI47_04085 0.55 -0.2 854,697 - IAI47_04085 0.55 +0.3 854,697 - IAI47_04085 0.55 +1.4 854,721 + IAI47_04085 0.57 +3.8 854,721 + IAI47_04085 0.57 +0.3 854,721 + IAI47_04085 0.57 -1.5 854,722 - IAI47_04085 0.57 -1.0 854,754 + IAI47_04085 0.59 +1.7 854,755 - IAI47_04085 0.60 -2.3 854,755 - IAI47_04085 0.60 +0.3 854,755 - IAI47_04085 0.60 +0.4 854,816 + IAI47_04085 0.64 +1.1 854,816 + IAI47_04085 0.64 +1.0 854,816 + IAI47_04085 0.64 +1.1 854,816 + IAI47_04085 0.64 +1.2 854,816 + IAI47_04085 0.64 -0.8 854,817 - IAI47_04085 0.64 +1.2 854,817 - IAI47_04085 0.64 -0.7 854,817 - IAI47_04085 0.64 -0.7 854,817 - IAI47_04085 0.64 -0.7 854,972 + IAI47_04085 0.76 -0.4 854,973 - IAI47_04085 0.76 +0.9 854,984 + IAI47_04085 0.77 -0.3 854,985 - IAI47_04085 0.77 +0.5 854,985 - IAI47_04085 0.77 +0.5 855,002 + IAI47_04085 0.78 +0.0 855,003 - IAI47_04085 0.78 -0.3 855,047 + IAI47_04085 0.81 -0.4 855,169 + -0.3 855,169 + -1.7 855,169 + +3.4 855,170 - -1.3 855,170 - +2.4 855,170 - +0.1 855,189 + +1.9 855,189 + -1.1 855,190 - -0.5 855,190 - -0.2 855,237 + +1.3 855,237 + +0.6 855,237 + -0.7 855,237 + -2.4 855,237 + +0.5 855,237 + +1.6 855,237 + +3.3 855,237 + -0.4 855,238 - +0.1 855,238 - -2.4 855,238 - +0.6 855,238 - -0.2 855,238 - -0.7 855,238 - -1.1 855,297 + -1.2 855,297 + -0.1 855,297 + +0.7 855,298 - -1.2 855,298 - -0.7 855,298 - +1.0 855,298 - -2.3 855,298 - -0.6 855,345 + -1.0 855,346 - -1.3 855,346 - +0.3 855,361 + +0.4 855,362 - -1.5 855,362 - +0.8 855,435 + -0.9 855,748 + IAI47_04090 0.33 -1.0 855,749 - IAI47_04090 0.33 -0.8 855,757 + IAI47_04090 0.34 +0.3 855,757 + IAI47_04090 0.34 -2.5 855,758 - IAI47_04090 0.34 -1.1 855,758 - IAI47_04090 0.34 +0.9 855,758 - IAI47_04090 0.34 +0.2 855,758 - IAI47_04090 0.34 -1.0 855,758 - IAI47_04090 0.34 +0.1 855,775 - IAI47_04090 0.35 -2.1 855,791 - IAI47_04090 0.36 -0.4 855,823 + IAI47_04090 0.39 -0.2 855,823 + IAI47_04090 0.39 -0.0 855,839 + IAI47_04090 0.40 +2.9 855,866 + IAI47_04090 0.42 +2.9 855,867 - IAI47_04090 0.42 +0.3 855,867 - IAI47_04090 0.42 -0.1 855,887 - IAI47_04090 0.43 +0.3 855,887 - IAI47_04090 0.43 +0.6 855,895 + IAI47_04090 0.44 -1.1 855,895 + IAI47_04090 0.44 +0.6 855,896 - IAI47_04090 0.44 +0.8 855,896 - IAI47_04090 0.44 +2.7 855,908 - IAI47_04090 0.45 +0.1 855,993 + IAI47_04090 0.51 +0.0 856,056 + IAI47_04090 0.56 -2.1 856,056 + IAI47_04090 0.56 -0.5 856,056 + IAI47_04090 0.56 +0.3 856,056 + IAI47_04090 0.56 -0.4 856,056 + IAI47_04090 0.56 +0.2 856,057 - IAI47_04090 0.56 -0.3 856,057 - IAI47_04090 0.56 +0.4 856,057 - IAI47_04090 0.56 -1.3 856,060 + IAI47_04090 0.56 -0.7 856,073 - IAI47_04090 0.57 -0.7 856,105 + IAI47_04090 0.60 +0.5 856,105 + IAI47_04090 0.60 -1.1 856,106 - IAI47_04090 0.60 +1.3 856,167 + IAI47_04090 0.64 -0.6 856,167 + IAI47_04090 0.64 -0.5 856,167 + IAI47_04090 0.64 +0.5 856,167 + IAI47_04090 0.64 +3.0 856,168 - IAI47_04090 0.64 -0.6 856,168 - IAI47_04090 0.64 -2.7 856,168 - IAI47_04090 0.64 +4.9 856,200 + IAI47_04090 0.67 +0.4 856,201 - IAI47_04090 0.67 -0.7 856,354 - IAI47_04090 0.78 -0.5 856,354 - IAI47_04090 0.78 -2.3 856,398 + IAI47_04090 0.82 -0.1 856,399 - IAI47_04090 0.82 -0.5 856,399 - IAI47_04090 0.82 +0.8 856,455 + IAI47_04090 0.86 +0.5 856,455 + IAI47_04090 0.86 -0.3 856,464 + IAI47_04090 0.87 +0.7 856,464 + IAI47_04090 0.87 -0.1 856,499 + IAI47_04090 0.89 -0.6 856,510 - +2.3 856,541 - -0.5 856,592 + +0.1 856,617 + -1.0 856,618 - -2.0 856,692 + +1.3 856,692 + +1.0 856,692 + +0.6 856,692 + +0.6 856,692 + +0.7 856,693 - -0.3 856,693 - -1.2 856,862 + IAI47_04095 0.23 +2.0 856,863 - IAI47_04095 0.23 +1.5 856,938 + IAI47_04095 0.33 -0.9 856,938 + IAI47_04095 0.33 +3.1 856,938 + IAI47_04095 0.33 -0.5 856,939 - IAI47_04095 0.33 -0.3 856,939 - IAI47_04095 0.33 -1.3 856,939 - IAI47_04095 0.33 -0.7 856,963 - IAI47_04095 0.36 -1.2 857,080 + IAI47_04095 0.51 -1.0 857,081 - IAI47_04095 0.51 -1.0 857,081 - IAI47_04095 0.51 -0.9 857,081 - IAI47_04095 0.51 +2.3 857,081 - IAI47_04095 0.51 -1.2 857,081 - IAI47_04095 0.51 +0.5 857,081 - IAI47_04095 0.51 -1.0 857,081 - IAI47_04095 0.51 -1.7 857,081 - IAI47_04095 0.51 -0.5 857,098 + IAI47_04095 0.53 -1.1 857,098 + IAI47_04095 0.53 -0.7 857,098 + IAI47_04095 0.53 +0.0 857,099 - IAI47_04095 0.53 -0.2 857,243 - IAI47_04095 0.72 -0.0 857,283 + IAI47_04095 0.77 +1.3 857,284 - IAI47_04095 0.77 +0.7 857,342 + IAI47_04095 0.85 +1.1 857,362 + IAI47_04095 0.88 -0.3 857,435 + +1.6 857,449 - -0.4 857,449 - +1.8 857,449 - +0.1 857,463 + +0.2 857,464 - -1.9 857,491 + +1.4 857,556 + +0.0 857,557 - -0.3 857,557 - +2.5 857,596 + IAI47_04100 0.13 +0.3 857,597 - IAI47_04100 0.14 -0.2
Or see this region's nucleotide sequence