Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10440

Experiment: copper (II) chloride 3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10430 and BWI76_RS10435 are separated by 44 nucleotidesBWI76_RS10435 and BWI76_RS10440 are separated by 7 nucleotidesBWI76_RS10440 and BWI76_RS10445 are separated by 46 nucleotidesBWI76_RS10445 and BWI76_RS10450 are separated by 239 nucleotides BWI76_RS10430: BWI76_RS10430 - thiol:disulfide interchange protein DsbG, at 2,204,877 to 2,205,635 _RS10430 BWI76_RS10435: BWI76_RS10435 - suppressor for copper-sensitivity C, at 2,205,680 to 2,206,402 _RS10435 BWI76_RS10440: BWI76_RS10440 - protein-disulfide reductase, at 2,206,410 to 2,208,434 _RS10440 BWI76_RS10445: BWI76_RS10445 - hypothetical protein, at 2,208,481 to 2,208,861 _RS10445 BWI76_RS10450: BWI76_RS10450 - tRNA-Tyr, at 2,209,101 to 2,209,175 _RS10450 Position (kb) 2206 2207 2208 2209Strain fitness (log2 ratio) -3 -2 -1 0 1at 2205.445 kb on + strand, within BWI76_RS10430at 2205.491 kb on - strand, within BWI76_RS10430at 2205.589 kb on - strandat 2205.739 kb on - strandat 2205.802 kb on + strand, within BWI76_RS10435at 2206.100 kb on + strand, within BWI76_RS10435at 2206.108 kb on - strand, within BWI76_RS10435at 2206.505 kb on + strandat 2206.705 kb on + strand, within BWI76_RS10440at 2206.789 kb on + strand, within BWI76_RS10440at 2207.397 kb on - strand, within BWI76_RS10440at 2207.489 kb on + strand, within BWI76_RS10440at 2207.570 kb on + strand, within BWI76_RS10440at 2207.661 kb on - strand, within BWI76_RS10440at 2207.890 kb on + strand, within BWI76_RS10440at 2207.993 kb on + strand, within BWI76_RS10440at 2208.134 kb on - strand, within BWI76_RS10440at 2208.164 kb on + strand, within BWI76_RS10440at 2208.269 kb on + strandat 2208.282 kb on - strandat 2208.357 kb on - strandat 2208.532 kb on + strand, within BWI76_RS10445at 2208.701 kb on - strand, within BWI76_RS10445at 2208.741 kb on - strand, within BWI76_RS10445at 2208.846 kb on + strandat 2208.854 kb on - strandat 2208.953 kb on + strandat 2208.953 kb on + strandat 2208.961 kb on - strandat 2208.961 kb on - strandat 2208.961 kb on - strandat 2208.963 kb on + strandat 2209.081 kb on - strandat 2209.112 kb on - strand, within BWI76_RS10450at 2209.112 kb on - strand, within BWI76_RS10450at 2209.145 kb on - strandat 2209.145 kb on - strand, within BWI76_RS10450at 2209.152 kb on + strand, within BWI76_RS10450at 2209.190 kb on + strandat 2209.237 kb on + strandat 2209.245 kb on - strandat 2209.326 kb on + strandat 2209.334 kb on - strandat 2209.337 kb on + strandat 2209.337 kb on + strandat 2209.337 kb on + strandat 2209.337 kb on + strandat 2209.345 kb on - strandat 2209.345 kb on - strandat 2209.345 kb on - strandat 2209.345 kb on - strandat 2209.347 kb on + strandat 2209.355 kb on - strandat 2209.395 kb on + strandat 2209.402 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM
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2,205,445 + BWI76_RS10430 0.75 +0.3
2,205,491 - BWI76_RS10430 0.81 -0.8
2,205,589 - -0.1
2,205,739 - -0.2
2,205,802 + BWI76_RS10435 0.17 -0.4
2,206,100 + BWI76_RS10435 0.58 -2.8
2,206,108 - BWI76_RS10435 0.59 -0.3
2,206,505 + -0.1
2,206,705 + BWI76_RS10440 0.15 -1.3
2,206,789 + BWI76_RS10440 0.19 -0.6
2,207,397 - BWI76_RS10440 0.49 -0.3
2,207,489 + BWI76_RS10440 0.53 -2.0
2,207,570 + BWI76_RS10440 0.57 -1.8
2,207,661 - BWI76_RS10440 0.62 -0.8
2,207,890 + BWI76_RS10440 0.73 -0.4
2,207,993 + BWI76_RS10440 0.78 -0.8
2,208,134 - BWI76_RS10440 0.85 -0.1
2,208,164 + BWI76_RS10440 0.87 -0.9
2,208,269 + -0.7
2,208,282 - -0.9
2,208,357 - -0.1
2,208,532 + BWI76_RS10445 0.13 -1.2
2,208,701 - BWI76_RS10445 0.58 -1.5
2,208,741 - BWI76_RS10445 0.68 -0.8
2,208,846 + -0.6
2,208,854 - -0.5
2,208,953 + +0.6
2,208,953 + -0.1
2,208,961 - -0.2
2,208,961 - -0.4
2,208,961 - +0.3
2,208,963 + +0.6
2,209,081 - +0.4
2,209,112 - BWI76_RS10450 0.15 -0.6
2,209,112 - BWI76_RS10450 0.15 +0.2
2,209,145 - -0.5
2,209,145 - BWI76_RS10450 0.59 -0.0
2,209,152 + BWI76_RS10450 0.68 -0.2
2,209,190 + -0.5
2,209,237 + +0.3
2,209,245 - +0.9
2,209,326 + +0.0
2,209,334 - +1.2
2,209,337 + +0.4
2,209,337 + -0.8
2,209,337 + -0.1
2,209,337 + -0.0
2,209,345 - -0.5
2,209,345 - +0.2
2,209,345 - +0.3
2,209,345 - -0.4
2,209,347 + -0.0
2,209,355 - +0.4
2,209,395 + -0.4
2,209,402 - -0.8

Or see this region's nucleotide sequence