Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_983 and Pf6N2E2_984 are separated by 232 nucleotides Pf6N2E2_984 and Pf6N2E2_985 are separated by 217 nucleotides Pf6N2E2_985 and Pf6N2E2_986 are separated by 315 nucleotides Pf6N2E2_986 and Pf6N2E2_987 overlap by 4 nucleotides Pf6N2E2_987 and Pf6N2E2_988 are separated by 247 nucleotides
Pf6N2E2_983 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 1,151,989 to 1,152,981
_983
Pf6N2E2_984 - FIG00553873: hypothetical protein, at 1,153,214 to 1,153,591
_984
Pf6N2E2_985 - Multidrug resistance transporter, Bcr/CflA family, at 1,153,809 to 1,154,945
_985
Pf6N2E2_986 - hypothetical protein, at 1,155,261 to 1,155,383
_986
Pf6N2E2_987 - hypothetical protein, at 1,155,380 to 1,155,616
_987
Pf6N2E2_988 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 1,155,864 to 1,156,721
_988
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.918 kb on - strand at 1152.966 kb on + strand at 1152.966 kb on + strand at 1152.995 kb on + strand at 1153.008 kb on - strand at 1153.130 kb on + strand at 1153.162 kb on + strand at 1153.162 kb on + strand at 1153.162 kb on + strand at 1153.191 kb on - strand at 1153.261 kb on + strand, within Pf6N2E2_984 at 1153.262 kb on - strand, within Pf6N2E2_984 at 1153.379 kb on + strand, within Pf6N2E2_984 at 1153.380 kb on - strand, within Pf6N2E2_984 at 1153.398 kb on + strand, within Pf6N2E2_984 at 1153.398 kb on + strand, within Pf6N2E2_984 at 1153.399 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.533 kb on + strand, within Pf6N2E2_984 at 1153.533 kb on + strand, within Pf6N2E2_984 at 1153.534 kb on - strand, within Pf6N2E2_984 at 1153.534 kb on - strand, within Pf6N2E2_984 at 1153.633 kb on - strand at 1153.633 kb on - strand at 1153.633 kb on - strand at 1153.786 kb on + strand at 1153.786 kb on + strand at 1153.786 kb on + strand at 1153.788 kb on + strand at 1153.789 kb on - strand at 1153.789 kb on - strand at 1153.789 kb on - strand at 1153.933 kb on + strand, within Pf6N2E2_985 at 1153.933 kb on + strand, within Pf6N2E2_985 at 1153.934 kb on - strand, within Pf6N2E2_985 at 1153.934 kb on - strand, within Pf6N2E2_985 at 1153.934 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.992 kb on + strand, within Pf6N2E2_985 at 1154.017 kb on - strand, within Pf6N2E2_985 at 1154.017 kb on - strand, within Pf6N2E2_985 at 1154.018 kb on + strand, within Pf6N2E2_985 at 1154.018 kb on + strand, within Pf6N2E2_985 at 1154.019 kb on - strand, within Pf6N2E2_985 at 1154.019 kb on - strand, within Pf6N2E2_985 at 1154.019 kb on - strand, within Pf6N2E2_985 at 1154.022 kb on + strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.100 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.214 kb on + strand, within Pf6N2E2_985 at 1154.272 kb on - strand, within Pf6N2E2_985 at 1154.337 kb on - strand, within Pf6N2E2_985 at 1154.339 kb on - strand, within Pf6N2E2_985 at 1154.339 kb on - strand, within Pf6N2E2_985 at 1154.348 kb on + strand, within Pf6N2E2_985 at 1154.348 kb on + strand, within Pf6N2E2_985 at 1154.348 kb on + strand, within Pf6N2E2_985 at 1154.349 kb on - strand, within Pf6N2E2_985 at 1154.416 kb on + strand, within Pf6N2E2_985 at 1154.416 kb on + strand, within Pf6N2E2_985 at 1154.511 kb on + strand, within Pf6N2E2_985 at 1154.512 kb on - strand, within Pf6N2E2_985 at 1154.527 kb on + strand, within Pf6N2E2_985 at 1154.527 kb on + strand, within Pf6N2E2_985 at 1154.527 kb on + strand, within Pf6N2E2_985 at 1154.528 kb on - strand, within Pf6N2E2_985 at 1154.544 kb on - strand, within Pf6N2E2_985 at 1154.673 kb on + strand, within Pf6N2E2_985 at 1154.706 kb on + strand, within Pf6N2E2_985 at 1154.707 kb on - strand, within Pf6N2E2_985 at 1154.865 kb on - strand at 1155.037 kb on + strand at 1155.038 kb on - strand at 1155.050 kb on + strand at 1155.122 kb on + strand at 1155.122 kb on + strand at 1155.122 kb on + strand at 1155.123 kb on - strand at 1155.141 kb on + strand at 1155.172 kb on + strand at 1155.173 kb on - strand at 1155.299 kb on + strand, within Pf6N2E2_986 at 1155.493 kb on + strand, within Pf6N2E2_987 at 1155.500 kb on + strand, within Pf6N2E2_987 at 1155.500 kb on + strand, within Pf6N2E2_987 at 1155.541 kb on - strand, within Pf6N2E2_987 at 1155.541 kb on - strand, within Pf6N2E2_987 at 1155.603 kb on + strand at 1155.604 kb on - strand at 1155.803 kb on + strand at 1155.865 kb on + strand at 1155.865 kb on + strand at 1155.865 kb on + strand at 1155.865 kb on + strand at 1155.866 kb on - strand at 1155.866 kb on - strand at 1155.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 1,152,917 + +1.7 1,152,917 + +1.7 1,152,917 + -2.1 1,152,917 + +1.6 1,152,917 + -0.9 1,152,917 + +0.7 1,152,918 - +0.3 1,152,966 + +1.4 1,152,966 + -1.0 1,152,995 + -1.0 1,153,008 - -1.4 1,153,130 + -3.3 1,153,162 + -0.1 1,153,162 + -0.6 1,153,162 + +1.0 1,153,191 - +3.0 1,153,261 + Pf6N2E2_984 0.12 -0.7 1,153,262 - Pf6N2E2_984 0.13 -1.2 1,153,379 + Pf6N2E2_984 0.44 +0.5 1,153,380 - Pf6N2E2_984 0.44 +1.2 1,153,398 + Pf6N2E2_984 0.49 +1.5 1,153,398 + Pf6N2E2_984 0.49 -1.1 1,153,399 - Pf6N2E2_984 0.49 +1.7 1,153,528 - Pf6N2E2_984 0.83 -0.6 1,153,528 - Pf6N2E2_984 0.83 +2.2 1,153,528 - Pf6N2E2_984 0.83 -1.2 1,153,528 - Pf6N2E2_984 0.83 +0.0 1,153,528 - Pf6N2E2_984 0.83 +1.6 1,153,528 - Pf6N2E2_984 0.83 +1.4 1,153,533 + Pf6N2E2_984 0.84 -2.5 1,153,533 + Pf6N2E2_984 0.84 -1.6 1,153,534 - Pf6N2E2_984 0.85 +0.8 1,153,534 - Pf6N2E2_984 0.85 -2.6 1,153,633 - -1.2 1,153,633 - +1.1 1,153,633 - +1.0 1,153,786 + -0.4 1,153,786 + +1.8 1,153,786 + -1.0 1,153,788 + -1.0 1,153,789 - +0.6 1,153,789 - +1.1 1,153,789 - -0.4 1,153,933 + Pf6N2E2_985 0.11 -2.7 1,153,933 + Pf6N2E2_985 0.11 -1.0 1,153,934 - Pf6N2E2_985 0.11 +1.7 1,153,934 - Pf6N2E2_985 0.11 +1.4 1,153,934 - Pf6N2E2_985 0.11 -0.4 1,153,990 - Pf6N2E2_985 0.16 +0.0 1,153,990 - Pf6N2E2_985 0.16 +1.0 1,153,990 - Pf6N2E2_985 0.16 -0.4 1,153,990 - Pf6N2E2_985 0.16 -0.5 1,153,992 + Pf6N2E2_985 0.16 -0.7 1,154,017 - Pf6N2E2_985 0.18 -1.5 1,154,017 - Pf6N2E2_985 0.18 -0.6 1,154,018 + Pf6N2E2_985 0.18 -2.9 1,154,018 + Pf6N2E2_985 0.18 -3.4 1,154,019 - Pf6N2E2_985 0.18 -1.1 1,154,019 - Pf6N2E2_985 0.18 -2.3 1,154,019 - Pf6N2E2_985 0.18 +0.3 1,154,022 + Pf6N2E2_985 0.19 +1.4 1,154,023 - Pf6N2E2_985 0.19 +1.8 1,154,023 - Pf6N2E2_985 0.19 -2.0 1,154,023 - Pf6N2E2_985 0.19 -0.1 1,154,023 - Pf6N2E2_985 0.19 +0.1 1,154,023 - Pf6N2E2_985 0.19 -1.4 1,154,023 - Pf6N2E2_985 0.19 -1.7 1,154,023 - Pf6N2E2_985 0.19 -2.4 1,154,100 - Pf6N2E2_985 0.26 +0.5 1,154,108 - Pf6N2E2_985 0.26 -2.9 1,154,108 - Pf6N2E2_985 0.26 +1.2 1,154,108 - Pf6N2E2_985 0.26 -0.1 1,154,108 - Pf6N2E2_985 0.26 -2.3 1,154,214 + Pf6N2E2_985 0.36 -1.9 1,154,272 - Pf6N2E2_985 0.41 -1.6 1,154,337 - Pf6N2E2_985 0.46 -0.3 1,154,339 - Pf6N2E2_985 0.47 +0.7 1,154,339 - Pf6N2E2_985 0.47 -1.0 1,154,348 + Pf6N2E2_985 0.47 -2.5 1,154,348 + Pf6N2E2_985 0.47 +0.3 1,154,348 + Pf6N2E2_985 0.47 -1.0 1,154,349 - Pf6N2E2_985 0.47 +1.3 1,154,416 + Pf6N2E2_985 0.53 +0.4 1,154,416 + Pf6N2E2_985 0.53 +0.2 1,154,511 + Pf6N2E2_985 0.62 -1.6 1,154,512 - Pf6N2E2_985 0.62 -0.8 1,154,527 + Pf6N2E2_985 0.63 +0.4 1,154,527 + Pf6N2E2_985 0.63 -0.2 1,154,527 + Pf6N2E2_985 0.63 +2.1 1,154,528 - Pf6N2E2_985 0.63 +0.0 1,154,544 - Pf6N2E2_985 0.65 +0.1 1,154,673 + Pf6N2E2_985 0.76 -1.6 1,154,706 + Pf6N2E2_985 0.79 -1.8 1,154,707 - Pf6N2E2_985 0.79 -1.9 1,154,865 - +0.8 1,155,037 + +1.2 1,155,038 - -1.9 1,155,050 + -0.6 1,155,122 + -0.7 1,155,122 + +2.8 1,155,122 + -0.4 1,155,123 - +0.2 1,155,141 + +1.6 1,155,172 + +0.8 1,155,173 - -0.7 1,155,299 + Pf6N2E2_986 0.31 +2.1 1,155,493 + Pf6N2E2_987 0.48 -1.2 1,155,500 + Pf6N2E2_987 0.51 -0.7 1,155,500 + Pf6N2E2_987 0.51 -1.2 1,155,541 - Pf6N2E2_987 0.68 +0.6 1,155,541 - Pf6N2E2_987 0.68 +0.0 1,155,603 + +2.1 1,155,604 - -1.9 1,155,803 + +1.3 1,155,865 + +1.8 1,155,865 + +0.1 1,155,865 + -2.0 1,155,865 + -2.1 1,155,866 - +0.3 1,155,866 - -0.7 1,155,866 - +0.5
Or see this region's nucleotide sequence