Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_90 and Pf6N2E2_91 are separated by 141 nucleotides Pf6N2E2_91 and Pf6N2E2_92 are separated by 106 nucleotides
Pf6N2E2_90 - Lipoprotein, putative, at 135,485 to 136,639
_90
Pf6N2E2_91 - Transcriptional regulator, LysR family, at 136,781 to 137,665
_91
Pf6N2E2_92 - hypothetical protein, at 137,772 to 138,221
_92
Position (kb) 136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 135.940 kb on - strand, within Pf6N2E2_90 at 136.015 kb on + strand, within Pf6N2E2_90 at 136.264 kb on + strand, within Pf6N2E2_90 at 136.352 kb on + strand, within Pf6N2E2_90 at 136.352 kb on + strand, within Pf6N2E2_90 at 136.369 kb on + strand, within Pf6N2E2_90 at 136.397 kb on + strand, within Pf6N2E2_90 at 136.547 kb on - strand at 136.567 kb on + strand at 136.568 kb on - strand at 136.613 kb on + strand at 136.613 kb on + strand at 136.679 kb on + strand at 136.679 kb on + strand at 136.679 kb on + strand at 136.679 kb on + strand at 136.680 kb on - strand at 136.680 kb on - strand at 136.865 kb on - strand at 136.865 kb on - strand at 136.866 kb on + strand at 137.163 kb on + strand, within Pf6N2E2_91 at 137.164 kb on - strand, within Pf6N2E2_91 at 137.285 kb on - strand, within Pf6N2E2_91 at 137.448 kb on + strand, within Pf6N2E2_91 at 137.448 kb on + strand, within Pf6N2E2_91 at 137.449 kb on - strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.454 kb on + strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.455 kb on - strand, within Pf6N2E2_91 at 137.679 kb on - strand at 137.703 kb on + strand at 137.780 kb on + strand at 137.780 kb on + strand at 137.861 kb on - strand, within Pf6N2E2_92 at 138.001 kb on + strand, within Pf6N2E2_92 at 138.002 kb on - strand, within Pf6N2E2_92 at 138.115 kb on + strand, within Pf6N2E2_92 at 138.116 kb on - strand, within Pf6N2E2_92 at 138.325 kb on + strand at 138.575 kb on + strand at 138.576 kb on - strand at 138.576 kb on - strand at 138.614 kb on + strand at 138.614 kb on + strand at 138.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 135,940 - Pf6N2E2_90 0.39 +0.4 136,015 + Pf6N2E2_90 0.46 +0.5 136,264 + Pf6N2E2_90 0.67 -0.4 136,352 + Pf6N2E2_90 0.75 -0.9 136,352 + Pf6N2E2_90 0.75 +1.0 136,369 + Pf6N2E2_90 0.77 +0.6 136,397 + Pf6N2E2_90 0.79 -1.3 136,547 - +1.5 136,567 + +0.9 136,568 - +1.9 136,613 + +0.3 136,613 + +0.2 136,679 + -0.9 136,679 + -0.6 136,679 + +1.3 136,679 + +0.2 136,680 - +1.4 136,680 - +0.5 136,865 - +2.0 136,865 - +0.0 136,866 + -3.5 137,163 + Pf6N2E2_91 0.43 +1.5 137,164 - Pf6N2E2_91 0.43 -1.8 137,285 - Pf6N2E2_91 0.57 -1.6 137,448 + Pf6N2E2_91 0.75 +2.3 137,448 + Pf6N2E2_91 0.75 -1.8 137,449 - Pf6N2E2_91 0.75 +1.1 137,454 + Pf6N2E2_91 0.76 -0.1 137,454 + Pf6N2E2_91 0.76 +1.5 137,454 + Pf6N2E2_91 0.76 +2.0 137,454 + Pf6N2E2_91 0.76 -2.5 137,454 + Pf6N2E2_91 0.76 +1.1 137,454 + Pf6N2E2_91 0.76 -1.0 137,454 + Pf6N2E2_91 0.76 -2.3 137,454 + Pf6N2E2_91 0.76 -2.4 137,454 + Pf6N2E2_91 0.76 -2.9 137,455 - Pf6N2E2_91 0.76 +1.8 137,455 - Pf6N2E2_91 0.76 -0.8 137,455 - Pf6N2E2_91 0.76 -1.4 137,455 - Pf6N2E2_91 0.76 +1.3 137,455 - Pf6N2E2_91 0.76 -0.3 137,455 - Pf6N2E2_91 0.76 +0.9 137,455 - Pf6N2E2_91 0.76 +2.0 137,455 - Pf6N2E2_91 0.76 +1.7 137,455 - Pf6N2E2_91 0.76 +0.4 137,679 - +0.2 137,703 + +2.1 137,780 + +0.6 137,780 + +1.5 137,861 - Pf6N2E2_92 0.20 -2.9 138,001 + Pf6N2E2_92 0.51 -0.1 138,002 - Pf6N2E2_92 0.51 -1.1 138,115 + Pf6N2E2_92 0.76 -0.8 138,116 - Pf6N2E2_92 0.76 +1.1 138,325 + +0.4 138,575 + -1.7 138,576 - +1.0 138,576 - -0.1 138,614 + -3.5 138,614 + +0.8 138,615 - -0.3
Or see this region's nucleotide sequence