Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_896 and Pf6N2E2_897 are separated by 21 nucleotides Pf6N2E2_897 and Pf6N2E2_898 are separated by 24 nucleotides
Pf6N2E2_896 - Regulatory protein, LysR:LysR, substrate-binding, at 1,049,160 to 1,050,077
_896
Pf6N2E2_897 - Maleate cis-trans isomerase (EC 5.2.1.1), at 1,050,099 to 1,050,851
_897
Pf6N2E2_898 - putative major facilitator superfamily (MFS) transporter, at 1,050,876 to 1,052,195
_898
Position (kb)
1050
1051 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1049.161 kb on + strand at 1049.161 kb on + strand at 1049.162 kb on - strand at 1049.227 kb on - strand at 1049.358 kb on + strand, within Pf6N2E2_896 at 1049.467 kb on + strand, within Pf6N2E2_896 at 1049.689 kb on + strand, within Pf6N2E2_896 at 1049.690 kb on - strand, within Pf6N2E2_896 at 1049.744 kb on + strand, within Pf6N2E2_896 at 1049.744 kb on + strand, within Pf6N2E2_896 at 1049.786 kb on - strand, within Pf6N2E2_896 at 1049.848 kb on - strand, within Pf6N2E2_896 at 1050.052 kb on - strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.080 kb on + strand at 1050.080 kb on + strand at 1050.080 kb on + strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.088 kb on + strand at 1050.089 kb on - strand at 1050.089 kb on - strand at 1050.135 kb on + strand at 1050.366 kb on + strand, within Pf6N2E2_897 at 1050.442 kb on + strand, within Pf6N2E2_897 at 1050.442 kb on + strand, within Pf6N2E2_897 at 1050.442 kb on + strand, within Pf6N2E2_897 at 1050.443 kb on - strand, within Pf6N2E2_897 at 1050.443 kb on - strand, within Pf6N2E2_897 at 1050.443 kb on - strand, within Pf6N2E2_897 at 1050.469 kb on + strand, within Pf6N2E2_897 at 1050.619 kb on + strand, within Pf6N2E2_897 at 1050.620 kb on - strand, within Pf6N2E2_897 at 1050.620 kb on - strand, within Pf6N2E2_897 at 1050.620 kb on - strand, within Pf6N2E2_897 at 1050.766 kb on + strand, within Pf6N2E2_897 at 1050.946 kb on + strand at 1050.947 kb on - strand at 1051.324 kb on + strand, within Pf6N2E2_898 at 1051.325 kb on - strand, within Pf6N2E2_898 at 1051.405 kb on + strand, within Pf6N2E2_898 at 1051.405 kb on + strand, within Pf6N2E2_898 at 1051.405 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.484 kb on + strand, within Pf6N2E2_898 at 1051.490 kb on - strand, within Pf6N2E2_898 at 1051.490 kb on - strand, within Pf6N2E2_898 at 1051.536 kb on - strand, within Pf6N2E2_898 at 1051.609 kb on + strand, within Pf6N2E2_898 at 1051.610 kb on - strand, within Pf6N2E2_898 at 1051.610 kb on - strand, within Pf6N2E2_898 at 1051.627 kb on + strand, within Pf6N2E2_898 at 1051.627 kb on + strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 1,049,161 + +1.6 1,049,161 + +0.1 1,049,162 - -1.4 1,049,227 - +1.0 1,049,358 + Pf6N2E2_896 0.22 +1.4 1,049,467 + Pf6N2E2_896 0.33 -1.5 1,049,689 + Pf6N2E2_896 0.58 +2.6 1,049,690 - Pf6N2E2_896 0.58 +0.1 1,049,744 + Pf6N2E2_896 0.64 -2.9 1,049,744 + Pf6N2E2_896 0.64 -1.0 1,049,786 - Pf6N2E2_896 0.68 -3.0 1,049,848 - Pf6N2E2_896 0.75 -0.7 1,050,052 - +1.6 1,050,077 + -2.3 1,050,077 + +1.5 1,050,077 + +1.6 1,050,077 + -0.2 1,050,077 + -1.4 1,050,077 + +1.6 1,050,077 + +2.6 1,050,077 + -0.7 1,050,077 + -1.4 1,050,078 - -0.9 1,050,078 - -3.0 1,050,078 - +0.0 1,050,078 - -1.4 1,050,078 - +0.3 1,050,078 - +0.4 1,050,078 - +1.8 1,050,078 - -0.2 1,050,078 - -1.5 1,050,078 - +1.8 1,050,080 + -0.4 1,050,080 + +1.4 1,050,080 + +3.1 1,050,081 - +0.3 1,050,081 - +0.6 1,050,081 - +0.6 1,050,081 - +0.8 1,050,081 - +1.3 1,050,088 + +0.1 1,050,089 - +1.6 1,050,089 - -3.2 1,050,135 + +2.1 1,050,366 + Pf6N2E2_897 0.35 -1.7 1,050,442 + Pf6N2E2_897 0.46 +1.3 1,050,442 + Pf6N2E2_897 0.46 +3.0 1,050,442 + Pf6N2E2_897 0.46 +1.0 1,050,443 - Pf6N2E2_897 0.46 +2.1 1,050,443 - Pf6N2E2_897 0.46 -1.2 1,050,443 - Pf6N2E2_897 0.46 +1.8 1,050,469 + Pf6N2E2_897 0.49 -1.4 1,050,619 + Pf6N2E2_897 0.69 +0.9 1,050,620 - Pf6N2E2_897 0.69 +1.3 1,050,620 - Pf6N2E2_897 0.69 +2.7 1,050,620 - Pf6N2E2_897 0.69 -0.2 1,050,766 + Pf6N2E2_897 0.89 +0.5 1,050,946 + +0.2 1,050,947 - +0.9 1,051,324 + Pf6N2E2_898 0.34 +1.4 1,051,325 - Pf6N2E2_898 0.34 -0.3 1,051,405 + Pf6N2E2_898 0.40 -0.2 1,051,405 + Pf6N2E2_898 0.40 +2.2 1,051,405 + Pf6N2E2_898 0.40 +2.9 1,051,441 + Pf6N2E2_898 0.43 +0.2 1,051,441 + Pf6N2E2_898 0.43 +0.8 1,051,441 + Pf6N2E2_898 0.43 -1.5 1,051,441 + Pf6N2E2_898 0.43 +0.5 1,051,441 + Pf6N2E2_898 0.43 -0.2 1,051,441 + Pf6N2E2_898 0.43 -1.7 1,051,442 - Pf6N2E2_898 0.43 -0.7 1,051,442 - Pf6N2E2_898 0.43 -2.2 1,051,442 - Pf6N2E2_898 0.43 -2.1 1,051,442 - Pf6N2E2_898 0.43 -2.4 1,051,442 - Pf6N2E2_898 0.43 +1.6 1,051,442 - Pf6N2E2_898 0.43 +0.2 1,051,442 - Pf6N2E2_898 0.43 -0.8 1,051,484 + Pf6N2E2_898 0.46 +1.2 1,051,490 - Pf6N2E2_898 0.47 +1.3 1,051,490 - Pf6N2E2_898 0.47 -1.5 1,051,536 - Pf6N2E2_898 0.50 -0.8 1,051,609 + Pf6N2E2_898 0.56 +0.3 1,051,610 - Pf6N2E2_898 0.56 -1.8 1,051,610 - Pf6N2E2_898 0.56 +1.0 1,051,627 + Pf6N2E2_898 0.57 -2.9 1,051,627 + Pf6N2E2_898 0.57 +2.1 1,051,628 - Pf6N2E2_898 0.57 -1.9 1,051,628 - Pf6N2E2_898 0.57 +1.3 1,051,628 - Pf6N2E2_898 0.57 -2.4 1,051,628 - Pf6N2E2_898 0.57 -1.7
Or see this region's nucleotide sequence