Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotides Pf6N2E2_700 and Pf6N2E2_701 overlap by 4 nucleotides Pf6N2E2_701 and Pf6N2E2_702 are separated by 2 nucleotides Pf6N2E2_702 and Pf6N2E2_703 overlap by 4 nucleotides Pf6N2E2_703 and Pf6N2E2_704 are separated by 190 nucleotides Pf6N2E2_704 and Pf6N2E2_705 are separated by 24 nucleotides
Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052
_699
Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557
_700
Pf6N2E2_701 - Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL, at 806,554 to 807,012
_701
Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193
_702
Pf6N2E2_703 - Cytochrome c55X precursor NirC, at 808,190 to 808,519
_703
Pf6N2E2_704 - Cytochrome c551/c552, at 808,710 to 809,018
_704
Pf6N2E2_705 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 809,043 to 810,722
_705
Position (kb)
807
808
809 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.224 kb on - strand, within Pf6N2E2_700 at 806.257 kb on - strand, within Pf6N2E2_700 at 806.708 kb on + strand, within Pf6N2E2_701 at 806.708 kb on + strand, within Pf6N2E2_701 at 806.709 kb on - strand, within Pf6N2E2_701 at 806.726 kb on + strand, within Pf6N2E2_701 at 806.726 kb on + strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 807.451 kb on + strand, within Pf6N2E2_702 at 807.452 kb on - strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.568 kb on + strand, within Pf6N2E2_702 at 807.569 kb on - strand, within Pf6N2E2_702 at 807.569 kb on - strand, within Pf6N2E2_702 at 807.569 kb on - strand, within Pf6N2E2_702 at 807.569 kb on - strand, within Pf6N2E2_702 at 807.569 kb on - strand, within Pf6N2E2_702 at 807.572 kb on - strand, within Pf6N2E2_702 at 807.572 kb on - strand, within Pf6N2E2_702 at 807.601 kb on + strand, within Pf6N2E2_702 at 807.601 kb on + strand, within Pf6N2E2_702 at 807.601 kb on + strand, within Pf6N2E2_702 at 807.601 kb on + strand, within Pf6N2E2_702 at 807.601 kb on + strand, within Pf6N2E2_702 at 807.602 kb on - strand, within Pf6N2E2_702 at 807.602 kb on - strand, within Pf6N2E2_702 at 807.814 kb on - strand, within Pf6N2E2_702 at 807.835 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.378 kb on - strand, within Pf6N2E2_703 at 808.378 kb on - strand, within Pf6N2E2_703 at 808.534 kb on + strand at 808.534 kb on + strand at 808.535 kb on - strand at 808.711 kb on + strand at 808.712 kb on - strand at 808.712 kb on - strand at 808.712 kb on - strand at 808.715 kb on + strand at 808.716 kb on - strand at 808.716 kb on - strand at 808.805 kb on + strand, within Pf6N2E2_704 at 808.806 kb on - strand, within Pf6N2E2_704 at 808.806 kb on - strand, within Pf6N2E2_704 at 808.901 kb on + strand, within Pf6N2E2_704 at 808.902 kb on - strand, within Pf6N2E2_704 at 809.010 kb on - strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.048 kb on - strand at 809.048 kb on - strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 806,223 + Pf6N2E2_700 0.35 +2.8 806,223 + Pf6N2E2_700 0.35 +1.8 806,223 + Pf6N2E2_700 0.35 +1.3 806,223 + Pf6N2E2_700 0.35 +3.3 806,223 + Pf6N2E2_700 0.35 -2.0 806,223 + Pf6N2E2_700 0.35 -1.6 806,224 - Pf6N2E2_700 0.35 -1.9 806,257 - Pf6N2E2_700 0.41 -0.5 806,708 + Pf6N2E2_701 0.34 +1.3 806,708 + Pf6N2E2_701 0.34 -3.0 806,709 - Pf6N2E2_701 0.34 +1.0 806,726 + Pf6N2E2_701 0.37 -1.5 806,726 + Pf6N2E2_701 0.37 -1.3 806,727 - Pf6N2E2_701 0.38 +2.7 806,727 - Pf6N2E2_701 0.38 -0.4 806,727 - Pf6N2E2_701 0.38 +0.2 806,727 - Pf6N2E2_701 0.38 -0.5 807,451 + Pf6N2E2_702 0.37 +0.8 807,452 - Pf6N2E2_702 0.37 +2.5 807,568 + Pf6N2E2_702 0.47 +1.5 807,568 + Pf6N2E2_702 0.47 -1.2 807,568 + Pf6N2E2_702 0.47 +0.0 807,568 + Pf6N2E2_702 0.47 -0.2 807,568 + Pf6N2E2_702 0.47 -1.8 807,568 + Pf6N2E2_702 0.47 -0.9 807,568 + Pf6N2E2_702 0.47 -1.6 807,568 + Pf6N2E2_702 0.47 -1.8 807,568 + Pf6N2E2_702 0.47 +2.1 807,568 + Pf6N2E2_702 0.47 +0.0 807,569 - Pf6N2E2_702 0.47 -1.7 807,569 - Pf6N2E2_702 0.47 -1.2 807,569 - Pf6N2E2_702 0.47 -2.0 807,569 - Pf6N2E2_702 0.47 -2.7 807,569 - Pf6N2E2_702 0.47 -1.1 807,572 - Pf6N2E2_702 0.47 -3.0 807,572 - Pf6N2E2_702 0.47 -2.2 807,601 + Pf6N2E2_702 0.50 +0.7 807,601 + Pf6N2E2_702 0.50 +1.5 807,601 + Pf6N2E2_702 0.50 -1.3 807,601 + Pf6N2E2_702 0.50 +0.2 807,601 + Pf6N2E2_702 0.50 +0.7 807,602 - Pf6N2E2_702 0.50 -1.1 807,602 - Pf6N2E2_702 0.50 -2.6 807,814 - Pf6N2E2_702 0.68 +4.4 807,835 + Pf6N2E2_702 0.70 -1.1 807,970 + Pf6N2E2_702 0.81 -0.4 807,970 + Pf6N2E2_702 0.81 +0.2 807,970 + Pf6N2E2_702 0.81 -1.3 807,970 + Pf6N2E2_702 0.81 +0.5 807,970 + Pf6N2E2_702 0.81 +1.4 807,971 - Pf6N2E2_702 0.81 -1.0 807,971 - Pf6N2E2_702 0.81 -2.4 807,971 - Pf6N2E2_702 0.81 -0.5 807,971 - Pf6N2E2_702 0.81 +1.7 808,377 + Pf6N2E2_703 0.57 -1.6 808,377 + Pf6N2E2_703 0.57 +0.3 808,377 + Pf6N2E2_703 0.57 +0.9 808,377 + Pf6N2E2_703 0.57 +1.4 808,377 + Pf6N2E2_703 0.57 -0.2 808,377 + Pf6N2E2_703 0.57 +2.4 808,378 - Pf6N2E2_703 0.57 -0.1 808,378 - Pf6N2E2_703 0.57 +0.1 808,534 + +0.6 808,534 + +2.0 808,535 - -1.1 808,711 + +0.1 808,712 - +1.4 808,712 - -2.1 808,712 - +0.2 808,715 + -1.5 808,716 - +1.4 808,716 - +0.7 808,805 + Pf6N2E2_704 0.31 -1.6 808,806 - Pf6N2E2_704 0.31 -2.1 808,806 - Pf6N2E2_704 0.31 +1.5 808,901 + Pf6N2E2_704 0.62 -1.4 808,902 - Pf6N2E2_704 0.62 +1.1 809,010 - -0.9 809,047 + -1.1 809,047 + +0.2 809,047 + -1.1 809,047 + -1.9 809,047 + -0.6 809,048 - +1.5 809,048 - -0.0 809,068 + -0.8 809,068 + +1.4 809,068 + -0.8 809,068 + +2.0 809,068 + +2.2 809,068 + -2.6 809,068 + -1.6 809,069 - +1.2
Or see this region's nucleotide sequence