Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_616 and Pf6N2E2_617 are separated by 143 nucleotides Pf6N2E2_617 and Pf6N2E2_618 overlap by 4 nucleotides Pf6N2E2_618 and Pf6N2E2_619 overlap by 4 nucleotides
Pf6N2E2_616 - hypothetical protein, at 699,541 to 699,666
_616
Pf6N2E2_617 - Probable glutathione S-transferase, at 699,810 to 700,412
_617
Pf6N2E2_618 - Exonuclease SbcC, at 700,409 to 704,050
_618
Pf6N2E2_619 - Exonuclease SbcD, at 704,047 to 705,291
_619
Position (kb) 700
701
702
703
704
705 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 699.527 kb on + strand at 699.527 kb on + strand at 699.528 kb on - strand at 699.578 kb on - strand, within Pf6N2E2_616 at 699.812 kb on - strand at 700.076 kb on - strand, within Pf6N2E2_617 at 700.076 kb on - strand, within Pf6N2E2_617 at 700.081 kb on + strand, within Pf6N2E2_617 at 700.081 kb on + strand, within Pf6N2E2_617 at 700.082 kb on - strand, within Pf6N2E2_617 at 700.394 kb on - strand at 700.394 kb on - strand at 700.394 kb on - strand at 700.559 kb on + strand at 700.697 kb on + strand at 700.697 kb on + strand at 700.697 kb on + strand at 700.698 kb on - strand at 700.771 kb on + strand at 700.771 kb on + strand at 700.771 kb on + strand at 700.772 kb on - strand at 700.772 kb on - strand at 700.782 kb on - strand, within Pf6N2E2_618 at 700.833 kb on + strand, within Pf6N2E2_618 at 700.908 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.991 kb on + strand, within Pf6N2E2_618 at 700.991 kb on + strand, within Pf6N2E2_618 at 701.345 kb on + strand, within Pf6N2E2_618 at 701.346 kb on - strand, within Pf6N2E2_618 at 701.346 kb on - strand, within Pf6N2E2_618 at 702.090 kb on + strand, within Pf6N2E2_618 at 702.090 kb on + strand, within Pf6N2E2_618 at 702.207 kb on + strand, within Pf6N2E2_618 at 702.579 kb on + strand, within Pf6N2E2_618 at 702.580 kb on - strand, within Pf6N2E2_618 at 703.244 kb on + strand, within Pf6N2E2_618 at 703.461 kb on + strand, within Pf6N2E2_618 at 703.462 kb on - strand, within Pf6N2E2_618 at 703.611 kb on + strand, within Pf6N2E2_618 at 704.130 kb on - strand at 704.234 kb on + strand, within Pf6N2E2_619 at 704.624 kb on + strand, within Pf6N2E2_619 at 704.624 kb on + strand, within Pf6N2E2_619 at 704.625 kb on - strand, within Pf6N2E2_619 at 704.770 kb on - strand, within Pf6N2E2_619
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 699,527 + +0.1 699,527 + -2.4 699,528 - -1.9 699,578 - Pf6N2E2_616 0.29 -1.9 699,812 - +0.2 700,076 - Pf6N2E2_617 0.44 -1.7 700,076 - Pf6N2E2_617 0.44 +1.6 700,081 + Pf6N2E2_617 0.45 -1.4 700,081 + Pf6N2E2_617 0.45 -1.3 700,082 - Pf6N2E2_617 0.45 +1.2 700,394 - +0.5 700,394 - +2.1 700,394 - -1.3 700,559 + -2.3 700,697 + -1.1 700,697 + -0.6 700,697 + -1.3 700,698 - -1.1 700,771 + +0.9 700,771 + +2.2 700,771 + +1.6 700,772 - +0.6 700,772 - +0.3 700,782 - Pf6N2E2_618 0.10 -0.1 700,833 + Pf6N2E2_618 0.12 -0.9 700,908 + Pf6N2E2_618 0.14 +0.7 700,917 + Pf6N2E2_618 0.14 +1.9 700,917 + Pf6N2E2_618 0.14 -0.1 700,917 + Pf6N2E2_618 0.14 -0.0 700,917 + Pf6N2E2_618 0.14 -0.2 700,917 + Pf6N2E2_618 0.14 -0.7 700,918 - Pf6N2E2_618 0.14 +0.9 700,918 - Pf6N2E2_618 0.14 -0.7 700,918 - Pf6N2E2_618 0.14 -2.5 700,991 + Pf6N2E2_618 0.16 -0.1 700,991 + Pf6N2E2_618 0.16 -1.6 701,345 + Pf6N2E2_618 0.26 +0.3 701,346 - Pf6N2E2_618 0.26 +1.7 701,346 - Pf6N2E2_618 0.26 -2.4 702,090 + Pf6N2E2_618 0.46 -2.1 702,090 + Pf6N2E2_618 0.46 -1.8 702,207 + Pf6N2E2_618 0.49 +0.7 702,579 + Pf6N2E2_618 0.60 +2.1 702,580 - Pf6N2E2_618 0.60 -0.6 703,244 + Pf6N2E2_618 0.78 -2.2 703,461 + Pf6N2E2_618 0.84 -0.3 703,462 - Pf6N2E2_618 0.84 -2.5 703,611 + Pf6N2E2_618 0.88 -0.3 704,130 - +4.0 704,234 + Pf6N2E2_619 0.15 -0.5 704,624 + Pf6N2E2_619 0.46 -1.1 704,624 + Pf6N2E2_619 0.46 +0.9 704,625 - Pf6N2E2_619 0.46 +2.2 704,770 - Pf6N2E2_619 0.58 +1.7
Or see this region's nucleotide sequence