Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5973 and Pf6N2E2_5974 are separated by 5 nucleotides Pf6N2E2_5974 and Pf6N2E2_5975 are separated by 187 nucleotides Pf6N2E2_5975 and Pf6N2E2_5976 are separated by 19 nucleotides Pf6N2E2_5976 and Pf6N2E2_5977 are separated by 49 nucleotides
Pf6N2E2_5973 - GCN5-related N-acetyltransferase (EC 2.3.1.57), at 6,791,345 to 6,791,827
_5973
Pf6N2E2_5974 - Uncharacterized glutathione S-transferase-like protein, at 6,791,833 to 6,792,465
_5974
Pf6N2E2_5975 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 6,792,653 to 6,793,633
_5975
Pf6N2E2_5976 - 2-dehydro-3-deoxyphosphogalactonate aldolase (EC 4.1.2.21), at 6,793,653 to 6,794,273
_5976
Pf6N2E2_5977 - Galactonate dehydratase (EC 4.2.1.6), at 6,794,323 to 6,795,471
_5977
Position (kb)
6792
6793
6794 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 6791.738 kb on + strand, within Pf6N2E2_5973 at 6791.739 kb on - strand, within Pf6N2E2_5973 at 6791.855 kb on + strand at 6791.856 kb on - strand at 6791.895 kb on - strand at 6791.895 kb on - strand at 6792.002 kb on + strand, within Pf6N2E2_5974 at 6792.003 kb on - strand, within Pf6N2E2_5974 at 6792.003 kb on - strand, within Pf6N2E2_5974 at 6792.109 kb on + strand, within Pf6N2E2_5974 at 6792.110 kb on - strand, within Pf6N2E2_5974 at 6792.243 kb on - strand, within Pf6N2E2_5974 at 6792.377 kb on + strand, within Pf6N2E2_5974 at 6792.477 kb on + strand at 6792.477 kb on + strand at 6792.504 kb on + strand at 6792.505 kb on - strand at 6792.505 kb on - strand at 6792.505 kb on - strand at 6792.505 kb on - strand at 6792.505 kb on - strand at 6792.505 kb on - strand at 6792.561 kb on - strand at 6792.578 kb on - strand at 6792.607 kb on + strand at 6792.608 kb on - strand at 6792.608 kb on - strand at 6792.608 kb on - strand at 6792.608 kb on - strand at 6792.609 kb on + strand at 6792.609 kb on + strand at 6792.610 kb on - strand at 6792.610 kb on - strand at 6792.705 kb on - strand at 6792.927 kb on - strand, within Pf6N2E2_5975 at 6792.969 kb on + strand, within Pf6N2E2_5975 at 6792.969 kb on + strand, within Pf6N2E2_5975 at 6792.970 kb on - strand, within Pf6N2E2_5975 at 6793.249 kb on + strand, within Pf6N2E2_5975 at 6793.249 kb on + strand, within Pf6N2E2_5975 at 6793.250 kb on - strand, within Pf6N2E2_5975 at 6793.250 kb on - strand, within Pf6N2E2_5975 at 6793.391 kb on + strand, within Pf6N2E2_5975 at 6793.744 kb on - strand, within Pf6N2E2_5976 at 6793.965 kb on + strand, within Pf6N2E2_5976 at 6793.965 kb on + strand, within Pf6N2E2_5976 at 6793.966 kb on - strand, within Pf6N2E2_5976 at 6794.029 kb on + strand, within Pf6N2E2_5976 at 6794.029 kb on + strand, within Pf6N2E2_5976 at 6794.029 kb on + strand, within Pf6N2E2_5976 at 6794.030 kb on - strand, within Pf6N2E2_5976 at 6794.030 kb on - strand, within Pf6N2E2_5976 at 6794.030 kb on - strand, within Pf6N2E2_5976 at 6794.030 kb on - strand, within Pf6N2E2_5976 at 6794.030 kb on - strand, within Pf6N2E2_5976 at 6794.130 kb on - strand, within Pf6N2E2_5976 at 6794.244 kb on + strand at 6794.244 kb on + strand at 6794.349 kb on + strand at 6794.473 kb on + strand, within Pf6N2E2_5977
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 6,791,738 + Pf6N2E2_5973 0.81 +0.6 6,791,739 - Pf6N2E2_5973 0.82 -1.8 6,791,855 + -1.8 6,791,856 - -2.8 6,791,895 - +1.0 6,791,895 - -1.2 6,792,002 + Pf6N2E2_5974 0.27 +0.6 6,792,003 - Pf6N2E2_5974 0.27 +0.4 6,792,003 - Pf6N2E2_5974 0.27 -1.5 6,792,109 + Pf6N2E2_5974 0.44 -0.4 6,792,110 - Pf6N2E2_5974 0.44 -3.0 6,792,243 - Pf6N2E2_5974 0.65 +0.7 6,792,377 + Pf6N2E2_5974 0.86 -0.6 6,792,477 + +0.1 6,792,477 + +0.9 6,792,504 + -1.5 6,792,505 - -2.4 6,792,505 - -1.6 6,792,505 - -2.2 6,792,505 - +0.7 6,792,505 - +2.1 6,792,505 - -1.5 6,792,561 - +0.5 6,792,578 - -0.4 6,792,607 + -2.3 6,792,608 - -2.9 6,792,608 - -1.5 6,792,608 - +2.4 6,792,608 - -0.6 6,792,609 + +0.4 6,792,609 + +1.0 6,792,610 - -2.7 6,792,610 - -1.4 6,792,705 - +0.4 6,792,927 - Pf6N2E2_5975 0.28 -1.0 6,792,969 + Pf6N2E2_5975 0.32 +0.1 6,792,969 + Pf6N2E2_5975 0.32 -1.0 6,792,970 - Pf6N2E2_5975 0.32 -0.6 6,793,249 + Pf6N2E2_5975 0.61 -1.6 6,793,249 + Pf6N2E2_5975 0.61 +1.6 6,793,250 - Pf6N2E2_5975 0.61 -2.5 6,793,250 - Pf6N2E2_5975 0.61 -2.2 6,793,391 + Pf6N2E2_5975 0.75 -1.0 6,793,744 - Pf6N2E2_5976 0.15 +0.6 6,793,965 + Pf6N2E2_5976 0.50 +5.5 6,793,965 + Pf6N2E2_5976 0.50 -1.4 6,793,966 - Pf6N2E2_5976 0.50 -1.6 6,794,029 + Pf6N2E2_5976 0.61 +3.8 6,794,029 + Pf6N2E2_5976 0.61 +3.1 6,794,029 + Pf6N2E2_5976 0.61 +2.9 6,794,030 - Pf6N2E2_5976 0.61 +0.9 6,794,030 - Pf6N2E2_5976 0.61 +1.3 6,794,030 - Pf6N2E2_5976 0.61 +0.6 6,794,030 - Pf6N2E2_5976 0.61 +2.0 6,794,030 - Pf6N2E2_5976 0.61 +0.3 6,794,130 - Pf6N2E2_5976 0.77 -2.4 6,794,244 + +2.2 6,794,244 + +1.0 6,794,349 + -2.0 6,794,473 + Pf6N2E2_5977 0.13 +0.7
Or see this region's nucleotide sequence