Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_592

Experiment: Bile salts 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_590 and Pf6N2E2_591 are separated by 327 nucleotidesPf6N2E2_591 and Pf6N2E2_592 overlap by 35 nucleotidesPf6N2E2_592 and Pf6N2E2_593 are separated by 86 nucleotidesPf6N2E2_593 and Pf6N2E2_594 are separated by 203 nucleotides Pf6N2E2_590 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 667,357 to 668,118 _590 Pf6N2E2_591 - Streptothricin acetyltransferase, Streptomyces lavendulae type, at 668,446 to 669,060 _591 Pf6N2E2_592 - Transcriptional regulators, LysR family, at 669,026 to 669,919 _592 Pf6N2E2_593 - FIG00958166: hypothetical protein, at 670,006 to 670,269 _593 Pf6N2E2_594 - FMN reductase, NADPH-dependent, at 670,473 to 671,012 _594 Position (kb) 669 670Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 668.128 kb on - strandat 668.128 kb on - strandat 668.154 kb on + strandat 668.154 kb on + strandat 668.154 kb on + strandat 668.154 kb on + strandat 668.155 kb on - strandat 668.155 kb on - strandat 668.155 kb on - strandat 668.158 kb on + strandat 668.158 kb on + strandat 668.158 kb on + strandat 668.158 kb on + strandat 668.158 kb on + strandat 668.159 kb on - strandat 668.159 kb on - strandat 668.159 kb on - strandat 668.159 kb on - strandat 668.159 kb on - strandat 668.159 kb on - strandat 668.201 kb on - strandat 668.285 kb on - strandat 668.329 kb on + strandat 668.329 kb on + strandat 668.330 kb on - strandat 668.330 kb on - strandat 668.330 kb on - strandat 668.445 kb on + strandat 668.446 kb on - strandat 668.446 kb on - strandat 668.446 kb on - strandat 668.446 kb on - strandat 668.515 kb on + strand, within Pf6N2E2_591at 668.515 kb on + strand, within Pf6N2E2_591at 668.516 kb on - strand, within Pf6N2E2_591at 668.517 kb on + strand, within Pf6N2E2_591at 668.517 kb on + strand, within Pf6N2E2_591at 668.518 kb on - strand, within Pf6N2E2_591at 668.518 kb on - strand, within Pf6N2E2_591at 668.518 kb on - strand, within Pf6N2E2_591at 668.518 kb on - strand, within Pf6N2E2_591at 668.518 kb on - strand, within Pf6N2E2_591at 668.518 kb on - strand, within Pf6N2E2_591at 668.626 kb on + strand, within Pf6N2E2_591at 668.626 kb on + strand, within Pf6N2E2_591at 668.627 kb on - strand, within Pf6N2E2_591at 668.692 kb on + strand, within Pf6N2E2_591at 668.693 kb on - strand, within Pf6N2E2_591at 668.693 kb on - strand, within Pf6N2E2_591at 668.917 kb on + strand, within Pf6N2E2_591at 668.918 kb on - strand, within Pf6N2E2_591at 668.968 kb on - strand, within Pf6N2E2_591at 669.121 kb on - strand, within Pf6N2E2_592at 669.302 kb on - strand, within Pf6N2E2_592at 669.344 kb on + strand, within Pf6N2E2_592at 669.345 kb on - strand, within Pf6N2E2_592at 669.345 kb on - strand, within Pf6N2E2_592at 669.382 kb on - strand, within Pf6N2E2_592at 669.382 kb on - strand, within Pf6N2E2_592at 669.382 kb on - strand, within Pf6N2E2_592at 669.634 kb on + strand, within Pf6N2E2_592at 669.635 kb on - strand, within Pf6N2E2_592at 669.657 kb on + strand, within Pf6N2E2_592at 669.705 kb on + strand, within Pf6N2E2_592at 669.912 kb on - strandat 669.997 kb on - strandat 670.015 kb on + strandat 670.143 kb on + strand, within Pf6N2E2_593at 670.143 kb on + strand, within Pf6N2E2_593at 670.144 kb on - strand, within Pf6N2E2_593at 670.144 kb on - strand, within Pf6N2E2_593at 670.144 kb on - strand, within Pf6N2E2_593at 670.145 kb on + strand, within Pf6N2E2_593at 670.145 kb on + strand, within Pf6N2E2_593at 670.145 kb on + strand, within Pf6N2E2_593at 670.146 kb on - strand, within Pf6N2E2_593at 670.146 kb on - strand, within Pf6N2E2_593at 670.146 kb on - strand, within Pf6N2E2_593at 670.259 kb on - strandat 670.275 kb on - strandat 670.343 kb on + strandat 670.344 kb on - strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.413 kb on - strandat 670.413 kb on - strandat 670.414 kb on + strandat 670.414 kb on + strandat 670.415 kb on - strandat 670.415 kb on - strandat 670.415 kb on - strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.419 kb on - strandat 670.460 kb on + strandat 670.460 kb on + strandat 670.460 kb on + strandat 670.461 kb on - strandat 670.461 kb on - strandat 670.461 kb on - strandat 670.467 kb on + strandat 670.596 kb on + strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.598 kb on + strand, within Pf6N2E2_594at 670.598 kb on + strand, within Pf6N2E2_594at 670.599 kb on - strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.632 kb on + strand, within Pf6N2E2_594at 670.632 kb on + strand, within Pf6N2E2_594at 670.632 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.693 kb on - strand, within Pf6N2E2_594

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Per-strain Table

Position Strand Gene LocusTag Fraction Bile salts 0.0125 M
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668,128 - +1.0
668,128 - -1.6
668,154 + +0.3
668,154 + +0.9
668,154 + +2.3
668,154 + +0.4
668,155 - +0.1
668,155 - -3.0
668,155 - +1.0
668,158 + +1.3
668,158 + +0.4
668,158 + +2.1
668,158 + +2.2
668,158 + +1.1
668,159 - +2.0
668,159 - -1.9
668,159 - +0.2
668,159 - -1.6
668,159 - -0.8
668,159 - -1.3
668,201 - +0.8
668,285 - -0.2
668,329 + +1.0
668,329 + +0.4
668,330 - -0.9
668,330 - +1.7
668,330 - +1.2
668,445 + +0.9
668,446 - -1.3
668,446 - +0.7
668,446 - +1.3
668,446 - +0.1
668,515 + Pf6N2E2_591 0.11 +0.7
668,515 + Pf6N2E2_591 0.11 +0.4
668,516 - Pf6N2E2_591 0.11 +2.4
668,517 + Pf6N2E2_591 0.12 +0.2
668,517 + Pf6N2E2_591 0.12 +0.5
668,518 - Pf6N2E2_591 0.12 +0.7
668,518 - Pf6N2E2_591 0.12 -2.4
668,518 - Pf6N2E2_591 0.12 -0.3
668,518 - Pf6N2E2_591 0.12 +0.6
668,518 - Pf6N2E2_591 0.12 -1.6
668,518 - Pf6N2E2_591 0.12 -1.3
668,626 + Pf6N2E2_591 0.29 -2.7
668,626 + Pf6N2E2_591 0.29 -2.2
668,627 - Pf6N2E2_591 0.29 +2.6
668,692 + Pf6N2E2_591 0.40 -0.4
668,693 - Pf6N2E2_591 0.40 -1.3
668,693 - Pf6N2E2_591 0.40 -1.4
668,917 + Pf6N2E2_591 0.77 +1.0
668,918 - Pf6N2E2_591 0.77 -1.0
668,968 - Pf6N2E2_591 0.85 +1.1
669,121 - Pf6N2E2_592 0.11 -2.0
669,302 - Pf6N2E2_592 0.31 -0.7
669,344 + Pf6N2E2_592 0.36 -1.0
669,345 - Pf6N2E2_592 0.36 +0.1
669,345 - Pf6N2E2_592 0.36 +0.6
669,382 - Pf6N2E2_592 0.40 -0.0
669,382 - Pf6N2E2_592 0.40 +1.0
669,382 - Pf6N2E2_592 0.40 -0.7
669,634 + Pf6N2E2_592 0.68 +0.3
669,635 - Pf6N2E2_592 0.68 -1.9
669,657 + Pf6N2E2_592 0.71 +0.7
669,705 + Pf6N2E2_592 0.76 +0.5
669,912 - +0.5
669,997 - +1.7
670,015 + +0.7
670,143 + Pf6N2E2_593 0.52 -0.9
670,143 + Pf6N2E2_593 0.52 +3.4
670,144 - Pf6N2E2_593 0.52 -0.9
670,144 - Pf6N2E2_593 0.52 +0.9
670,144 - Pf6N2E2_593 0.52 +1.8
670,145 + Pf6N2E2_593 0.53 -1.1
670,145 + Pf6N2E2_593 0.53 -0.9
670,145 + Pf6N2E2_593 0.53 +1.3
670,146 - Pf6N2E2_593 0.53 +0.2
670,146 - Pf6N2E2_593 0.53 +2.4
670,146 - Pf6N2E2_593 0.53 +1.0
670,259 - -0.3
670,275 - +1.7
670,343 + +3.4
670,344 - -1.9
670,412 + -0.5
670,412 + -0.9
670,412 + -1.3
670,412 + +2.3
670,412 + -1.1
670,412 + +1.7
670,413 - +1.5
670,413 - -0.9
670,414 + +0.3
670,414 + -1.9
670,415 - -3.0
670,415 - -0.3
670,415 - -0.4
670,418 + -0.6
670,418 + +2.0
670,418 + -2.3
670,418 + +1.7
670,418 + +0.1
670,419 - +1.1
670,460 + -1.5
670,460 + -1.1
670,460 + +0.5
670,461 - -1.2
670,461 - -1.5
670,461 - -2.0
670,467 + +0.9
670,596 + Pf6N2E2_594 0.23 +0.4
670,597 - Pf6N2E2_594 0.23 +2.7
670,597 - Pf6N2E2_594 0.23 +1.8
670,597 - Pf6N2E2_594 0.23 -3.2
670,597 - Pf6N2E2_594 0.23 +2.8
670,597 - Pf6N2E2_594 0.23 +0.4
670,598 + Pf6N2E2_594 0.23 +1.1
670,598 + Pf6N2E2_594 0.23 +2.3
670,599 - Pf6N2E2_594 0.23 +0.1
670,615 + Pf6N2E2_594 0.26 -0.1
670,615 + Pf6N2E2_594 0.26 -0.9
670,615 + Pf6N2E2_594 0.26 -1.1
670,615 + Pf6N2E2_594 0.26 +0.2
670,632 + Pf6N2E2_594 0.29 +1.7
670,632 + Pf6N2E2_594 0.29 -0.2
670,632 + Pf6N2E2_594 0.29 -0.2
670,692 + Pf6N2E2_594 0.41 +1.9
670,692 + Pf6N2E2_594 0.41 +1.7
670,692 + Pf6N2E2_594 0.41 +0.2
670,692 + Pf6N2E2_594 0.41 -0.2
670,693 - Pf6N2E2_594 0.41 -0.5

Or see this region's nucleotide sequence