Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5915 and Pf6N2E2_5916 overlap by 4 nucleotides Pf6N2E2_5916 and Pf6N2E2_5917 are separated by 27 nucleotides
Pf6N2E2_5915 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 6,733,310 to 6,734,752
_5915
Pf6N2E2_5916 - Flp pilus assembly protein TadB, at 6,734,749 to 6,735,735
_5916
Pf6N2E2_5917 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 6,735,763 to 6,736,728
_5917
Position (kb)
6734
6735
6736 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6734.196 kb on + strand, within Pf6N2E2_5915 at 6734.197 kb on - strand, within Pf6N2E2_5915 at 6734.197 kb on - strand, within Pf6N2E2_5915 at 6734.197 kb on - strand, within Pf6N2E2_5915 at 6734.267 kb on - strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.403 kb on + strand, within Pf6N2E2_5915 at 6734.404 kb on - strand, within Pf6N2E2_5915 at 6734.404 kb on - strand, within Pf6N2E2_5915 at 6734.807 kb on + strand at 6734.808 kb on - strand at 6734.871 kb on + strand, within Pf6N2E2_5916 at 6734.872 kb on - strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.944 kb on + strand, within Pf6N2E2_5916 at 6734.945 kb on - strand, within Pf6N2E2_5916 at 6735.037 kb on + strand, within Pf6N2E2_5916 at 6735.098 kb on + strand, within Pf6N2E2_5916 at 6735.098 kb on + strand, within Pf6N2E2_5916 at 6735.099 kb on - strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.352 kb on + strand, within Pf6N2E2_5916 at 6735.353 kb on - strand, within Pf6N2E2_5916 at 6735.622 kb on + strand, within Pf6N2E2_5916 at 6735.622 kb on + strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.623 kb on - strand, within Pf6N2E2_5916 at 6735.878 kb on + strand, within Pf6N2E2_5917 at 6735.879 kb on - strand, within Pf6N2E2_5917 at 6736.096 kb on + strand, within Pf6N2E2_5917 at 6736.097 kb on - strand, within Pf6N2E2_5917 at 6736.237 kb on + strand at 6736.237 kb on + strand, within Pf6N2E2_5917 at 6736.238 kb on - strand, within Pf6N2E2_5917 at 6736.238 kb on - strand, within Pf6N2E2_5917 at 6736.238 kb on - strand, within Pf6N2E2_5917 at 6736.533 kb on + strand, within Pf6N2E2_5917 at 6736.561 kb on + strand, within Pf6N2E2_5917 at 6736.722 kb on + strand at 6736.722 kb on + strand at 6736.722 kb on + strand at 6736.723 kb on - strand at 6736.723 kb on - strand at 6736.723 kb on - strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.726 kb on + strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand at 6736.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 6,734,196 + Pf6N2E2_5915 0.61 -0.1 6,734,197 - Pf6N2E2_5915 0.61 +1.2 6,734,197 - Pf6N2E2_5915 0.61 +1.0 6,734,197 - Pf6N2E2_5915 0.61 +1.9 6,734,267 - Pf6N2E2_5915 0.66 +1.0 6,734,403 + Pf6N2E2_5915 0.76 +2.3 6,734,403 + Pf6N2E2_5915 0.76 +0.5 6,734,403 + Pf6N2E2_5915 0.76 +1.0 6,734,403 + Pf6N2E2_5915 0.76 -2.5 6,734,404 - Pf6N2E2_5915 0.76 -1.5 6,734,404 - Pf6N2E2_5915 0.76 -1.3 6,734,807 + -3.0 6,734,808 - -1.0 6,734,871 + Pf6N2E2_5916 0.12 +1.4 6,734,872 - Pf6N2E2_5916 0.12 -2.7 6,734,944 + Pf6N2E2_5916 0.20 +2.2 6,734,944 + Pf6N2E2_5916 0.20 +2.7 6,734,944 + Pf6N2E2_5916 0.20 +2.5 6,734,945 - Pf6N2E2_5916 0.20 +0.8 6,735,037 + Pf6N2E2_5916 0.29 +1.2 6,735,098 + Pf6N2E2_5916 0.35 -2.8 6,735,098 + Pf6N2E2_5916 0.35 +0.6 6,735,099 - Pf6N2E2_5916 0.35 +0.1 6,735,352 + Pf6N2E2_5916 0.61 -0.5 6,735,352 + Pf6N2E2_5916 0.61 +0.4 6,735,352 + Pf6N2E2_5916 0.61 +2.2 6,735,353 - Pf6N2E2_5916 0.61 +0.2 6,735,622 + Pf6N2E2_5916 0.88 -1.5 6,735,622 + Pf6N2E2_5916 0.88 -2.7 6,735,623 - Pf6N2E2_5916 0.89 +2.1 6,735,623 - Pf6N2E2_5916 0.89 +2.0 6,735,623 - Pf6N2E2_5916 0.89 -1.8 6,735,623 - Pf6N2E2_5916 0.89 -1.3 6,735,623 - Pf6N2E2_5916 0.89 -0.4 6,735,878 + Pf6N2E2_5917 0.12 +0.9 6,735,879 - Pf6N2E2_5917 0.12 +1.6 6,736,096 + Pf6N2E2_5917 0.34 +1.6 6,736,097 - Pf6N2E2_5917 0.35 -1.9 6,736,237 + -0.0 6,736,237 + Pf6N2E2_5917 0.49 -0.7 6,736,238 - Pf6N2E2_5917 0.49 -1.3 6,736,238 - Pf6N2E2_5917 0.49 +0.1 6,736,238 - Pf6N2E2_5917 0.49 -2.4 6,736,533 + Pf6N2E2_5917 0.80 -2.5 6,736,561 + Pf6N2E2_5917 0.83 -0.8 6,736,722 + -2.0 6,736,722 + -1.3 6,736,722 + -1.1 6,736,723 - -1.8 6,736,723 - -1.9 6,736,723 - -1.2 6,736,726 + -0.8 6,736,726 + +0.6 6,736,726 + -0.5 6,736,726 + +1.0 6,736,726 + +1.3 6,736,726 + +0.1 6,736,727 - -0.8 6,736,727 - +2.6 6,736,727 - +1.4 6,736,727 - +1.2 6,736,727 - -2.1 6,736,727 - +1.6
Or see this region's nucleotide sequence