Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_560 and Pf6N2E2_561 overlap by 4 nucleotides Pf6N2E2_561 and Pf6N2E2_562 overlap by 4 nucleotides
Pf6N2E2_560 - IcmF-related protein, at 630,724 to 634,548
_560
Pf6N2E2_561 - Uncharacterized protein ImpA, at 634,545 to 635,627
_561
Pf6N2E2_562 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 635,624 to 636,577
_562
Position (kb) 634
635
636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 633.719 kb on - strand, within Pf6N2E2_560 at 633.766 kb on + strand, within Pf6N2E2_560 at 633.767 kb on - strand, within Pf6N2E2_560 at 633.767 kb on - strand, within Pf6N2E2_560 at 633.779 kb on - strand, within Pf6N2E2_560 at 633.964 kb on - strand, within Pf6N2E2_560 at 633.987 kb on - strand, within Pf6N2E2_560 at 634.261 kb on + strand at 634.333 kb on + strand at 634.334 kb on - strand at 634.823 kb on + strand, within Pf6N2E2_561 at 634.824 kb on - strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.242 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.486 kb on + strand, within Pf6N2E2_561 at 635.708 kb on + strand at 635.708 kb on + strand at 636.036 kb on + strand, within Pf6N2E2_562 at 636.069 kb on + strand, within Pf6N2E2_562 at 636.070 kb on - strand, within Pf6N2E2_562 at 636.076 kb on + strand, within Pf6N2E2_562 at 636.222 kb on - strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.534 kb on + strand at 636.535 kb on - strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.591 kb on - strand at 636.591 kb on - strand at 636.612 kb on + strand at 636.613 kb on - strand at 636.613 kb on - strand at 636.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 633,719 - Pf6N2E2_560 0.78 -1.6 633,766 + Pf6N2E2_560 0.80 -2.6 633,767 - Pf6N2E2_560 0.80 +2.3 633,767 - Pf6N2E2_560 0.80 +0.9 633,779 - Pf6N2E2_560 0.80 -1.2 633,964 - Pf6N2E2_560 0.85 +1.2 633,987 - Pf6N2E2_560 0.85 +1.0 634,261 + -1.4 634,333 + -1.8 634,334 - +1.8 634,823 + Pf6N2E2_561 0.26 -3.2 634,824 - Pf6N2E2_561 0.26 -2.3 635,241 + Pf6N2E2_561 0.64 -1.1 635,241 + Pf6N2E2_561 0.64 -1.6 635,242 - Pf6N2E2_561 0.64 -1.1 635,266 - Pf6N2E2_561 0.67 -0.3 635,266 - Pf6N2E2_561 0.67 +0.1 635,459 - Pf6N2E2_561 0.84 +2.4 635,459 - Pf6N2E2_561 0.84 +0.3 635,486 + Pf6N2E2_561 0.87 -0.8 635,708 + -2.2 635,708 + -2.3 636,036 + Pf6N2E2_562 0.43 -0.9 636,069 + Pf6N2E2_562 0.47 -2.4 636,070 - Pf6N2E2_562 0.47 -0.3 636,076 + Pf6N2E2_562 0.47 +0.8 636,222 - Pf6N2E2_562 0.63 -1.1 636,335 + Pf6N2E2_562 0.75 +0.7 636,335 + Pf6N2E2_562 0.75 +1.5 636,335 + Pf6N2E2_562 0.75 -1.3 636,335 + Pf6N2E2_562 0.75 +1.3 636,336 - Pf6N2E2_562 0.75 -2.4 636,336 - Pf6N2E2_562 0.75 +0.4 636,336 - Pf6N2E2_562 0.75 -3.0 636,534 + -2.1 636,535 - +1.7 636,590 + -1.7 636,590 + +0.7 636,590 + +1.8 636,590 + -1.5 636,590 + +0.8 636,591 - +0.6 636,591 - -0.2 636,612 + -2.1 636,613 - -2.5 636,613 - -0.9 636,627 + +1.2
Or see this region's nucleotide sequence