Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5545 and Pf6N2E2_5546 are separated by 1 nucleotides Pf6N2E2_5546 and Pf6N2E2_6157 are separated by 63 nucleotides Pf6N2E2_6157 and Pf6N2E2_5547 are separated by 152 nucleotides Pf6N2E2_5547 and Pf6N2E2_5548 are separated by 97 nucleotides
Pf6N2E2_5545 - Queuosine Biosynthesis QueE Radical SAM, at 6,359,873 to 6,360,520
_5545
Pf6N2E2_5546 - Queuosine Biosynthesis QueC ATPase, at 6,360,522 to 6,361,214
_5546
Pf6N2E2_6157 - tRNA-Lys-TTT, at 6,361,278 to 6,361,350
_6157
Pf6N2E2_5547 - hypothetical protein, at 6,361,503 to 6,362,933
_5547
Pf6N2E2_5548 - Cyanate transport protein CynX, at 6,363,031 to 6,364,218
_5548
Position (kb)
6361
6362
6363 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6360.521 kb on + strand at 6360.521 kb on + strand at 6360.522 kb on - strand at 6360.522 kb on - strand at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.631 kb on - strand, within Pf6N2E2_5546 at 6360.649 kb on - strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.819 kb on + strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.820 kb on - strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.833 kb on + strand, within Pf6N2E2_5546 at 6360.841 kb on + strand, within Pf6N2E2_5546 at 6360.842 kb on - strand, within Pf6N2E2_5546 at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.424 kb on + strand at 6361.425 kb on - strand at 6361.668 kb on - strand, within Pf6N2E2_5547 at 6361.746 kb on + strand, within Pf6N2E2_5547 at 6361.746 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.752 kb on + strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.753 kb on - strand, within Pf6N2E2_5547 at 6361.769 kb on - strand, within Pf6N2E2_5547 at 6361.769 kb on - strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.851 kb on + strand, within Pf6N2E2_5547 at 6361.852 kb on - strand, within Pf6N2E2_5547 at 6361.852 kb on - strand, within Pf6N2E2_5547 at 6361.918 kb on + strand, within Pf6N2E2_5547 at 6361.919 kb on - strand, within Pf6N2E2_5547 at 6361.950 kb on + strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6361.951 kb on - strand, within Pf6N2E2_5547 at 6362.008 kb on + strand, within Pf6N2E2_5547 at 6362.008 kb on + strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.009 kb on - strand, within Pf6N2E2_5547 at 6362.034 kb on + strand, within Pf6N2E2_5547 at 6362.034 kb on + strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.035 kb on - strand, within Pf6N2E2_5547 at 6362.089 kb on + strand, within Pf6N2E2_5547 at 6362.089 kb on + strand, within Pf6N2E2_5547 at 6362.090 kb on - strand, within Pf6N2E2_5547 at 6362.090 kb on - strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.122 kb on + strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.123 kb on - strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.130 kb on + strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.131 kb on - strand, within Pf6N2E2_5547 at 6362.175 kb on + strand, within Pf6N2E2_5547 at 6362.257 kb on - strand, within Pf6N2E2_5547 at 6362.348 kb on + strand, within Pf6N2E2_5547 at 6362.349 kb on - strand, within Pf6N2E2_5547 at 6362.374 kb on - strand, within Pf6N2E2_5547 at 6362.374 kb on - strand, within Pf6N2E2_5547 at 6362.374 kb on - strand, within Pf6N2E2_5547 at 6362.374 kb on - strand, within Pf6N2E2_5547 at 6362.473 kb on + strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.474 kb on - strand, within Pf6N2E2_5547 at 6362.520 kb on + strand, within Pf6N2E2_5547 at 6362.695 kb on + strand, within Pf6N2E2_5547 at 6362.696 kb on - strand, within Pf6N2E2_5547 at 6362.796 kb on - strand at 6362.797 kb on + strand at 6362.797 kb on + strand at 6362.798 kb on - strand at 6362.798 kb on - strand at 6362.798 kb on - strand at 6362.798 kb on - strand at 6362.803 kb on + strand at 6362.803 kb on + strand at 6362.803 kb on + strand at 6362.803 kb on + strand at 6362.803 kb on + strand at 6362.804 kb on - strand at 6362.804 kb on - strand at 6362.804 kb on - strand at 6362.804 kb on - strand at 6362.841 kb on + strand at 6362.867 kb on - strand at 6362.938 kb on + strand at 6362.938 kb on + strand at 6362.939 kb on - strand at 6362.939 kb on - strand at 6362.947 kb on + strand at 6362.970 kb on + strand at 6362.972 kb on + strand at 6362.973 kb on - strand at 6362.973 kb on - strand at 6362.973 kb on - strand at 6362.976 kb on - strand at 6362.995 kb on + strand at 6362.995 kb on + strand at 6362.996 kb on - strand at 6363.009 kb on - strand at 6363.009 kb on - strand at 6363.009 kb on - strand at 6363.009 kb on - strand at 6363.073 kb on + strand at 6363.074 kb on - strand at 6363.074 kb on - strand at 6363.074 kb on - strand at 6363.278 kb on - strand, within Pf6N2E2_5548 at 6363.278 kb on - strand, within Pf6N2E2_5548 at 6363.512 kb on + strand, within Pf6N2E2_5548 at 6363.512 kb on + strand, within Pf6N2E2_5548 at 6363.512 kb on + strand, within Pf6N2E2_5548 at 6363.512 kb on + strand, within Pf6N2E2_5548 at 6363.513 kb on - strand, within Pf6N2E2_5548 at 6363.513 kb on - strand, within Pf6N2E2_5548 at 6363.513 kb on - strand, within Pf6N2E2_5548 at 6363.545 kb on + strand, within Pf6N2E2_5548 at 6363.546 kb on - strand, within Pf6N2E2_5548 at 6363.546 kb on - strand, within Pf6N2E2_5548 at 6363.797 kb on + strand, within Pf6N2E2_5548 at 6363.797 kb on + strand, within Pf6N2E2_5548 at 6363.797 kb on + strand, within Pf6N2E2_5548 at 6363.797 kb on + strand, within Pf6N2E2_5548 at 6363.797 kb on + strand, within Pf6N2E2_5548 at 6363.797 kb on + strand, within Pf6N2E2_5548 at 6363.797 kb on + strand, within Pf6N2E2_5548 at 6363.798 kb on - strand, within Pf6N2E2_5548 at 6363.798 kb on - strand, within Pf6N2E2_5548 at 6363.798 kb on - strand, within Pf6N2E2_5548 at 6363.798 kb on - strand, within Pf6N2E2_5548 at 6363.929 kb on + strand, within Pf6N2E2_5548 at 6363.929 kb on + strand, within Pf6N2E2_5548 at 6363.930 kb on - strand, within Pf6N2E2_5548 at 6363.930 kb on - strand, within Pf6N2E2_5548
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 6,360,521 + +0.4 6,360,521 + -1.0 6,360,522 - -0.2 6,360,522 - +2.3 6,360,631 - Pf6N2E2_5546 0.16 -1.7 6,360,631 - Pf6N2E2_5546 0.16 +2.5 6,360,631 - Pf6N2E2_5546 0.16 +2.0 6,360,649 - Pf6N2E2_5546 0.18 +1.2 6,360,819 + Pf6N2E2_5546 0.43 -1.0 6,360,819 + Pf6N2E2_5546 0.43 -1.2 6,360,819 + Pf6N2E2_5546 0.43 +1.2 6,360,819 + Pf6N2E2_5546 0.43 +2.7 6,360,819 + Pf6N2E2_5546 0.43 +0.8 6,360,819 + Pf6N2E2_5546 0.43 -0.6 6,360,819 + Pf6N2E2_5546 0.43 -0.5 6,360,819 + Pf6N2E2_5546 0.43 -1.4 6,360,819 + Pf6N2E2_5546 0.43 -1.7 6,360,819 + Pf6N2E2_5546 0.43 +1.4 6,360,819 + Pf6N2E2_5546 0.43 -1.6 6,360,820 - Pf6N2E2_5546 0.43 +1.0 6,360,820 - Pf6N2E2_5546 0.43 -0.2 6,360,820 - Pf6N2E2_5546 0.43 -0.7 6,360,820 - Pf6N2E2_5546 0.43 -0.6 6,360,820 - Pf6N2E2_5546 0.43 -1.6 6,360,820 - Pf6N2E2_5546 0.43 -2.2 6,360,833 + Pf6N2E2_5546 0.45 +0.9 6,360,833 + Pf6N2E2_5546 0.45 +1.1 6,360,841 + Pf6N2E2_5546 0.46 +1.2 6,360,842 - Pf6N2E2_5546 0.46 +0.7 6,361,424 + -2.6 6,361,424 + +0.2 6,361,424 + -2.8 6,361,425 - +0.1 6,361,668 - Pf6N2E2_5547 0.12 +1.3 6,361,746 + Pf6N2E2_5547 0.17 +2.0 6,361,746 + Pf6N2E2_5547 0.17 -1.8 6,361,752 + Pf6N2E2_5547 0.17 -0.4 6,361,752 + Pf6N2E2_5547 0.17 -1.6 6,361,752 + Pf6N2E2_5547 0.17 -1.7 6,361,752 + Pf6N2E2_5547 0.17 -1.4 6,361,752 + Pf6N2E2_5547 0.17 +1.0 6,361,753 - Pf6N2E2_5547 0.17 +0.2 6,361,753 - Pf6N2E2_5547 0.17 +1.4 6,361,753 - Pf6N2E2_5547 0.17 +0.6 6,361,753 - Pf6N2E2_5547 0.17 -1.9 6,361,753 - Pf6N2E2_5547 0.17 +0.7 6,361,753 - Pf6N2E2_5547 0.17 +0.8 6,361,769 - Pf6N2E2_5547 0.19 -2.8 6,361,769 - Pf6N2E2_5547 0.19 -0.1 6,361,851 + Pf6N2E2_5547 0.24 +1.0 6,361,851 + Pf6N2E2_5547 0.24 -2.7 6,361,851 + Pf6N2E2_5547 0.24 +0.4 6,361,851 + Pf6N2E2_5547 0.24 +2.2 6,361,852 - Pf6N2E2_5547 0.24 -2.4 6,361,852 - Pf6N2E2_5547 0.24 -1.4 6,361,918 + Pf6N2E2_5547 0.29 +0.8 6,361,919 - Pf6N2E2_5547 0.29 -2.5 6,361,950 + Pf6N2E2_5547 0.31 +0.6 6,361,951 - Pf6N2E2_5547 0.31 -1.8 6,361,951 - Pf6N2E2_5547 0.31 +1.5 6,361,951 - Pf6N2E2_5547 0.31 -0.1 6,362,008 + Pf6N2E2_5547 0.35 +1.7 6,362,008 + Pf6N2E2_5547 0.35 +0.1 6,362,009 - Pf6N2E2_5547 0.35 -1.6 6,362,009 - Pf6N2E2_5547 0.35 -2.6 6,362,009 - Pf6N2E2_5547 0.35 -0.6 6,362,009 - Pf6N2E2_5547 0.35 -0.9 6,362,009 - Pf6N2E2_5547 0.35 -1.2 6,362,034 + Pf6N2E2_5547 0.37 -0.1 6,362,034 + Pf6N2E2_5547 0.37 -3.5 6,362,035 - Pf6N2E2_5547 0.37 +2.4 6,362,035 - Pf6N2E2_5547 0.37 +0.2 6,362,035 - Pf6N2E2_5547 0.37 -1.8 6,362,089 + Pf6N2E2_5547 0.41 +1.4 6,362,089 + Pf6N2E2_5547 0.41 +2.3 6,362,090 - Pf6N2E2_5547 0.41 -0.5 6,362,090 - Pf6N2E2_5547 0.41 +3.0 6,362,122 + Pf6N2E2_5547 0.43 +0.3 6,362,122 + Pf6N2E2_5547 0.43 +2.3 6,362,122 + Pf6N2E2_5547 0.43 -0.2 6,362,122 + Pf6N2E2_5547 0.43 +1.1 6,362,122 + Pf6N2E2_5547 0.43 -1.4 6,362,122 + Pf6N2E2_5547 0.43 +2.0 6,362,123 - Pf6N2E2_5547 0.43 -1.9 6,362,123 - Pf6N2E2_5547 0.43 +0.6 6,362,123 - Pf6N2E2_5547 0.43 +2.2 6,362,123 - Pf6N2E2_5547 0.43 +0.4 6,362,123 - Pf6N2E2_5547 0.43 -1.4 6,362,123 - Pf6N2E2_5547 0.43 +1.7 6,362,123 - Pf6N2E2_5547 0.43 -1.2 6,362,123 - Pf6N2E2_5547 0.43 -1.9 6,362,130 + Pf6N2E2_5547 0.44 +1.8 6,362,130 + Pf6N2E2_5547 0.44 -2.6 6,362,130 + Pf6N2E2_5547 0.44 -1.2 6,362,130 + Pf6N2E2_5547 0.44 -0.8 6,362,131 - Pf6N2E2_5547 0.44 +0.3 6,362,131 - Pf6N2E2_5547 0.44 +1.3 6,362,131 - Pf6N2E2_5547 0.44 -3.5 6,362,175 + Pf6N2E2_5547 0.47 -0.6 6,362,257 - Pf6N2E2_5547 0.53 +2.8 6,362,348 + Pf6N2E2_5547 0.59 +0.2 6,362,349 - Pf6N2E2_5547 0.59 -1.0 6,362,374 - Pf6N2E2_5547 0.61 -0.6 6,362,374 - Pf6N2E2_5547 0.61 +1.8 6,362,374 - Pf6N2E2_5547 0.61 +1.2 6,362,374 - Pf6N2E2_5547 0.61 -1.5 6,362,473 + Pf6N2E2_5547 0.68 -1.0 6,362,474 - Pf6N2E2_5547 0.68 -0.8 6,362,474 - Pf6N2E2_5547 0.68 +2.8 6,362,474 - Pf6N2E2_5547 0.68 -0.3 6,362,474 - Pf6N2E2_5547 0.68 +0.9 6,362,474 - Pf6N2E2_5547 0.68 +2.2 6,362,474 - Pf6N2E2_5547 0.68 -0.5 6,362,474 - Pf6N2E2_5547 0.68 +0.7 6,362,474 - Pf6N2E2_5547 0.68 -0.6 6,362,520 + Pf6N2E2_5547 0.71 +0.9 6,362,695 + Pf6N2E2_5547 0.83 +0.7 6,362,696 - Pf6N2E2_5547 0.83 -0.4 6,362,796 - -2.6 6,362,797 + -0.4 6,362,797 + -3.0 6,362,798 - +1.5 6,362,798 - -1.0 6,362,798 - +1.6 6,362,798 - -2.4 6,362,803 + -0.8 6,362,803 + -0.8 6,362,803 + +0.6 6,362,803 + +0.3 6,362,803 + +1.3 6,362,804 - -0.1 6,362,804 - -0.1 6,362,804 - -1.5 6,362,804 - -0.0 6,362,841 + -1.5 6,362,867 - -1.0 6,362,938 + -1.6 6,362,938 + -0.0 6,362,939 - -0.8 6,362,939 - +2.8 6,362,947 + -0.4 6,362,970 + +1.3 6,362,972 + -1.6 6,362,973 - -0.6 6,362,973 - +0.4 6,362,973 - -1.4 6,362,976 - +2.1 6,362,995 + -0.7 6,362,995 + -0.6 6,362,996 - -1.2 6,363,009 - +0.3 6,363,009 - -0.5 6,363,009 - -0.6 6,363,009 - -1.4 6,363,073 + -0.4 6,363,074 - +1.1 6,363,074 - +0.3 6,363,074 - -1.3 6,363,278 - Pf6N2E2_5548 0.21 +0.0 6,363,278 - Pf6N2E2_5548 0.21 +1.6 6,363,512 + Pf6N2E2_5548 0.40 -2.6 6,363,512 + Pf6N2E2_5548 0.40 +2.0 6,363,512 + Pf6N2E2_5548 0.40 -2.2 6,363,512 + Pf6N2E2_5548 0.40 +0.9 6,363,513 - Pf6N2E2_5548 0.41 +0.5 6,363,513 - Pf6N2E2_5548 0.41 +0.7 6,363,513 - Pf6N2E2_5548 0.41 -1.8 6,363,545 + Pf6N2E2_5548 0.43 -0.3 6,363,546 - Pf6N2E2_5548 0.43 +1.1 6,363,546 - Pf6N2E2_5548 0.43 +1.4 6,363,797 + Pf6N2E2_5548 0.64 -0.0 6,363,797 + Pf6N2E2_5548 0.64 +1.7 6,363,797 + Pf6N2E2_5548 0.64 -0.7 6,363,797 + Pf6N2E2_5548 0.64 -1.6 6,363,797 + Pf6N2E2_5548 0.64 -1.1 6,363,797 + Pf6N2E2_5548 0.64 +0.4 6,363,797 + Pf6N2E2_5548 0.64 -0.6 6,363,798 - Pf6N2E2_5548 0.65 -0.0 6,363,798 - Pf6N2E2_5548 0.65 +1.9 6,363,798 - Pf6N2E2_5548 0.65 +0.3 6,363,798 - Pf6N2E2_5548 0.65 +1.1 6,363,929 + Pf6N2E2_5548 0.76 +2.1 6,363,929 + Pf6N2E2_5548 0.76 +1.0 6,363,930 - Pf6N2E2_5548 0.76 +0.2 6,363,930 - Pf6N2E2_5548 0.76 +1.3
Or see this region's nucleotide sequence