Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5222 and Pf6N2E2_5223 overlap by 7 nucleotides Pf6N2E2_5223 and Pf6N2E2_5224 overlap by 8 nucleotides Pf6N2E2_5224 and Pf6N2E2_5225 are separated by 114 nucleotides Pf6N2E2_5225 and Pf6N2E2_5226 are separated by 59 nucleotides
Pf6N2E2_5222 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 6,021,016 to 6,021,846
_5222
Pf6N2E2_5223 - Molybdopterin biosynthesis protein MoeB, at 6,021,840 to 6,022,595
_5223
Pf6N2E2_5224 - Glutamate racemase (EC 5.1.1.3), at 6,022,588 to 6,023,379
_5224
Pf6N2E2_5225 - Lipid A 3-O-deacylase, at 6,023,494 to 6,024,012
_5225
Pf6N2E2_5226 - FIG00955589: hypothetical protein, at 6,024,072 to 6,024,377
_5226
Position (kb)
6022
6023
6024 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6022.271 kb on + strand, within Pf6N2E2_5223 at 6022.353 kb on + strand, within Pf6N2E2_5223 at 6022.365 kb on + strand, within Pf6N2E2_5223 at 6022.365 kb on + strand, within Pf6N2E2_5223 at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.339 kb on + strand at 6023.436 kb on - strand at 6023.437 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.467 kb on - strand at 6023.467 kb on - strand at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.611 kb on + strand, within Pf6N2E2_5225 at 6023.612 kb on - strand, within Pf6N2E2_5225 at 6023.695 kb on + strand, within Pf6N2E2_5225 at 6023.695 kb on + strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.698 kb on - strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.938 kb on + strand, within Pf6N2E2_5225 at 6023.938 kb on + strand, within Pf6N2E2_5225 at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.976 kb on - strand at 6023.976 kb on - strand at 6024.008 kb on + strand at 6024.011 kb on - strand at 6024.011 kb on - strand at 6024.055 kb on + strand at 6024.055 kb on + strand at 6024.055 kb on + strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.164 kb on - strand, within Pf6N2E2_5226 at 6024.164 kb on - strand, within Pf6N2E2_5226
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 6,021,978 + Pf6N2E2_5223 0.18 +0.3 6,021,978 + Pf6N2E2_5223 0.18 +1.6 6,021,978 + Pf6N2E2_5223 0.18 -0.4 6,021,978 + Pf6N2E2_5223 0.18 -0.9 6,022,271 + Pf6N2E2_5223 0.57 -0.7 6,022,353 + Pf6N2E2_5223 0.68 +1.0 6,022,365 + Pf6N2E2_5223 0.69 +0.1 6,022,365 + Pf6N2E2_5223 0.69 +1.8 6,023,334 + -2.3 6,023,334 + -2.5 6,023,334 + -3.4 6,023,339 + -3.4 6,023,436 - -1.1 6,023,437 + -2.5 6,023,466 + +0.2 6,023,466 + +1.5 6,023,466 + +0.3 6,023,466 + +1.5 6,023,466 + +0.8 6,023,467 - +0.3 6,023,467 - -1.7 6,023,548 + Pf6N2E2_5225 0.10 +2.0 6,023,548 + Pf6N2E2_5225 0.10 -0.4 6,023,548 + Pf6N2E2_5225 0.10 +0.3 6,023,548 + Pf6N2E2_5225 0.10 -1.9 6,023,548 + Pf6N2E2_5225 0.10 -0.9 6,023,548 + Pf6N2E2_5225 0.10 -1.4 6,023,548 + Pf6N2E2_5225 0.10 -0.8 6,023,548 + Pf6N2E2_5225 0.10 -0.5 6,023,548 + Pf6N2E2_5225 0.10 +2.3 6,023,549 - Pf6N2E2_5225 0.11 -1.4 6,023,549 - Pf6N2E2_5225 0.11 -2.3 6,023,549 - Pf6N2E2_5225 0.11 -2.7 6,023,611 + Pf6N2E2_5225 0.23 -0.8 6,023,612 - Pf6N2E2_5225 0.23 -1.2 6,023,695 + Pf6N2E2_5225 0.39 +0.7 6,023,695 + Pf6N2E2_5225 0.39 +0.6 6,023,696 - Pf6N2E2_5225 0.39 -0.2 6,023,696 - Pf6N2E2_5225 0.39 +0.2 6,023,696 - Pf6N2E2_5225 0.39 +0.6 6,023,696 - Pf6N2E2_5225 0.39 -1.8 6,023,696 - Pf6N2E2_5225 0.39 -0.3 6,023,697 + Pf6N2E2_5225 0.39 -0.4 6,023,697 + Pf6N2E2_5225 0.39 +0.8 6,023,697 + Pf6N2E2_5225 0.39 -0.5 6,023,697 + Pf6N2E2_5225 0.39 -0.9 6,023,697 + Pf6N2E2_5225 0.39 -1.2 6,023,697 + Pf6N2E2_5225 0.39 -3.1 6,023,697 + Pf6N2E2_5225 0.39 -1.5 6,023,697 + Pf6N2E2_5225 0.39 -2.2 6,023,698 - Pf6N2E2_5225 0.39 -0.6 6,023,701 + Pf6N2E2_5225 0.40 -2.5 6,023,701 + Pf6N2E2_5225 0.40 -2.7 6,023,701 + Pf6N2E2_5225 0.40 -2.2 6,023,701 + Pf6N2E2_5225 0.40 +1.0 6,023,701 + Pf6N2E2_5225 0.40 +0.6 6,023,702 - Pf6N2E2_5225 0.40 +0.8 6,023,702 - Pf6N2E2_5225 0.40 -2.6 6,023,702 - Pf6N2E2_5225 0.40 -0.1 6,023,791 + Pf6N2E2_5225 0.57 -0.7 6,023,791 + Pf6N2E2_5225 0.57 -2.0 6,023,791 + Pf6N2E2_5225 0.57 -1.9 6,023,791 + Pf6N2E2_5225 0.57 -1.4 6,023,791 + Pf6N2E2_5225 0.57 -2.0 6,023,791 + Pf6N2E2_5225 0.57 -1.0 6,023,791 + Pf6N2E2_5225 0.57 -1.8 6,023,792 - Pf6N2E2_5225 0.57 +0.1 6,023,792 - Pf6N2E2_5225 0.57 +0.2 6,023,792 - Pf6N2E2_5225 0.57 +0.7 6,023,792 - Pf6N2E2_5225 0.57 -3.0 6,023,938 + Pf6N2E2_5225 0.86 -1.5 6,023,938 + Pf6N2E2_5225 0.86 +0.2 6,023,975 + -1.2 6,023,975 + +0.6 6,023,975 + -0.1 6,023,975 + +1.4 6,023,976 - +2.4 6,023,976 - -0.2 6,024,008 + +0.2 6,024,011 - -1.1 6,024,011 - +0.3 6,024,055 + -2.8 6,024,055 + -1.0 6,024,055 + +1.9 6,024,056 - -1.4 6,024,056 - -0.7 6,024,056 - -1.3 6,024,056 - +2.1 6,024,164 - Pf6N2E2_5226 0.30 -2.9 6,024,164 - Pf6N2E2_5226 0.30 -1.1
Or see this region's nucleotide sequence