Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_515 and Pf6N2E2_516 are separated by 35 nucleotides Pf6N2E2_516 and Pf6N2E2_517 are separated by 148 nucleotides
Pf6N2E2_515 - malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) <small>(from data)</small>, at 584,020 to 585,522
_515
Pf6N2E2_516 - Inosose isomerase (EC 5.3.99.-), at 585,558 to 586,379
_516
Pf6N2E2_517 - Epi-inositol hydrolase (EC 3.7.1.-) <small>(from data)</small>, at 586,528 to 588,459
_517
Position (kb) 585
586
587 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.723 kb on - strand, within Pf6N2E2_515 at 584.723 kb on - strand, within Pf6N2E2_515 at 585.082 kb on - strand, within Pf6N2E2_515 at 585.082 kb on - strand, within Pf6N2E2_515 at 585.163 kb on + strand, within Pf6N2E2_515 at 585.163 kb on + strand, within Pf6N2E2_515 at 585.164 kb on - strand, within Pf6N2E2_515 at 585.164 kb on - strand, within Pf6N2E2_515 at 585.330 kb on - strand, within Pf6N2E2_515 at 585.415 kb on + strand at 585.440 kb on - strand at 585.448 kb on + strand at 585.520 kb on + strand at 585.521 kb on - strand at 585.521 kb on - strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.727 kb on + strand, within Pf6N2E2_516 at 585.728 kb on - strand, within Pf6N2E2_516 at 585.829 kb on - strand, within Pf6N2E2_516 at 585.969 kb on + strand, within Pf6N2E2_516 at 585.970 kb on - strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.064 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.441 kb on - strand at 586.441 kb on - strand at 586.585 kb on + strand at 586.585 kb on + strand at 586.586 kb on - strand at 586.780 kb on + strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.868 kb on + strand, within Pf6N2E2_517 at 586.869 kb on - strand at 586.869 kb on - strand, within Pf6N2E2_517 at 586.869 kb on - strand, within Pf6N2E2_517 at 586.885 kb on + strand, within Pf6N2E2_517 at 586.982 kb on - strand, within Pf6N2E2_517 at 587.111 kb on - strand, within Pf6N2E2_517 at 587.126 kb on - strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 584,722 + Pf6N2E2_515 0.47 -0.5 584,722 + Pf6N2E2_515 0.47 -0.0 584,722 + Pf6N2E2_515 0.47 +2.0 584,722 + Pf6N2E2_515 0.47 +1.7 584,723 - Pf6N2E2_515 0.47 -0.1 584,723 - Pf6N2E2_515 0.47 +1.7 585,082 - Pf6N2E2_515 0.71 +1.1 585,082 - Pf6N2E2_515 0.71 +0.5 585,163 + Pf6N2E2_515 0.76 +1.4 585,163 + Pf6N2E2_515 0.76 +0.1 585,164 - Pf6N2E2_515 0.76 +1.0 585,164 - Pf6N2E2_515 0.76 -1.0 585,330 - Pf6N2E2_515 0.87 -0.4 585,415 + +2.1 585,440 - -1.6 585,448 + +0.7 585,520 + +0.9 585,521 - -0.3 585,521 - +2.5 585,553 + -0.9 585,553 + -2.5 585,553 + +1.1 585,554 - +1.3 585,554 - -3.2 585,554 - -1.8 585,554 - +2.4 585,554 - -2.0 585,554 - +0.7 585,554 - +1.1 585,554 - +1.3 585,727 + Pf6N2E2_516 0.21 +2.3 585,728 - Pf6N2E2_516 0.21 -2.5 585,829 - Pf6N2E2_516 0.33 -3.1 585,969 + Pf6N2E2_516 0.50 -1.3 585,970 - Pf6N2E2_516 0.50 -2.6 586,063 + Pf6N2E2_516 0.61 +0.9 586,063 + Pf6N2E2_516 0.61 +1.5 586,063 + Pf6N2E2_516 0.61 +1.7 586,064 - Pf6N2E2_516 0.62 -1.9 586,279 - Pf6N2E2_516 0.88 +4.1 586,279 - Pf6N2E2_516 0.88 +0.9 586,279 - Pf6N2E2_516 0.88 +0.5 586,441 - -0.9 586,441 - -0.6 586,585 + +1.3 586,585 + -0.5 586,586 - +1.1 586,780 + Pf6N2E2_517 0.13 -1.4 586,781 - Pf6N2E2_517 0.13 +0.2 586,781 - Pf6N2E2_517 0.13 +0.4 586,868 + Pf6N2E2_517 0.18 +1.8 586,869 - +1.1 586,869 - Pf6N2E2_517 0.18 +0.9 586,869 - Pf6N2E2_517 0.18 +1.2 586,885 + Pf6N2E2_517 0.18 -0.9 586,982 - Pf6N2E2_517 0.23 +1.8 587,111 - Pf6N2E2_517 0.30 -0.6 587,126 - Pf6N2E2_517 0.31 -0.3 587,183 + Pf6N2E2_517 0.34 -2.1 587,183 + Pf6N2E2_517 0.34 +1.8
Or see this region's nucleotide sequence