Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_493 and Pf6N2E2_494 are separated by 149 nucleotides Pf6N2E2_494 and Pf6N2E2_495 are separated by 29 nucleotides Pf6N2E2_495 and Pf6N2E2_496 are separated by 183 nucleotides
Pf6N2E2_493 - YD repeat protein, at 559,785 to 560,831
_493
Pf6N2E2_494 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 560,981 to 561,418
_494
Pf6N2E2_495 - YD repeat protein, at 561,448 to 562,563
_495
Pf6N2E2_496 - Dihydropyrimidine dehydrogenase (NADP(+)) (EC 1.3.1.2) (from data) , at 562,747 to 564,021
_496
Position (kb)
561
562
563 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 560.452 kb on + strand, within Pf6N2E2_493 at 560.452 kb on + strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.729 kb on - strand at 560.729 kb on - strand at 560.729 kb on - strand at 560.894 kb on - strand at 560.896 kb on + strand at 560.908 kb on + strand at 560.909 kb on - strand at 560.909 kb on - strand at 560.927 kb on - strand at 560.946 kb on + strand at 560.946 kb on + strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.949 kb on + strand at 560.949 kb on + strand at 560.949 kb on + strand at 560.950 kb on - strand at 560.950 kb on - strand at 560.971 kb on + strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.973 kb on + strand at 560.973 kb on + strand at 561.065 kb on + strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.180 kb on - strand, within Pf6N2E2_494 at 561.453 kb on - strand at 561.573 kb on + strand, within Pf6N2E2_495 at 561.637 kb on + strand, within Pf6N2E2_495 at 561.834 kb on + strand, within Pf6N2E2_495 at 562.001 kb on + strand, within Pf6N2E2_495 at 562.085 kb on + strand, within Pf6N2E2_495 at 562.184 kb on + strand, within Pf6N2E2_495 at 562.184 kb on + strand, within Pf6N2E2_495 at 562.607 kb on + strand at 562.625 kb on + strand at 562.628 kb on + strand at 562.640 kb on + strand at 562.640 kb on + strand at 562.640 kb on + strand at 562.640 kb on + strand at 562.641 kb on - strand at 562.764 kb on - strand at 562.867 kb on - strand at 562.892 kb on - strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.899 kb on - strand, within Pf6N2E2_496 at 562.902 kb on + strand, within Pf6N2E2_496 at 562.902 kb on + strand, within Pf6N2E2_496 at 562.902 kb on + strand, within Pf6N2E2_496 at 562.903 kb on - strand at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 563.021 kb on + strand, within Pf6N2E2_496 at 563.021 kb on + strand, within Pf6N2E2_496 at 563.022 kb on - strand, within Pf6N2E2_496 at 563.022 kb on - strand, within Pf6N2E2_496 at 563.022 kb on - strand, within Pf6N2E2_496 at 563.036 kb on + strand, within Pf6N2E2_496 at 563.036 kb on + strand, within Pf6N2E2_496 at 563.036 kb on + strand, within Pf6N2E2_496 at 563.522 kb on + strand, within Pf6N2E2_496 at 563.522 kb on + strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.555 kb on + strand, within Pf6N2E2_496 at 563.555 kb on + strand, within Pf6N2E2_496 at 563.556 kb on - strand, within Pf6N2E2_496 at 563.556 kb on - strand, within Pf6N2E2_496
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 560,452 + Pf6N2E2_493 0.64 -0.3 560,452 + Pf6N2E2_493 0.64 -2.0 560,701 - Pf6N2E2_493 0.87 +1.8 560,701 - Pf6N2E2_493 0.87 +0.3 560,701 - Pf6N2E2_493 0.87 -2.3 560,701 - Pf6N2E2_493 0.87 -1.7 560,729 - +1.6 560,729 - +1.1 560,729 - +0.1 560,894 - +2.0 560,896 + -0.5 560,908 + +0.8 560,909 - -2.2 560,909 - -3.1 560,927 - +1.9 560,946 + +1.1 560,946 + -0.3 560,947 - +0.9 560,947 - -0.4 560,947 - +0.8 560,947 - -0.3 560,947 - -2.1 560,949 + -1.1 560,949 + +2.0 560,949 + +0.5 560,950 - -0.3 560,950 - -2.3 560,971 + -0.1 560,972 - +1.1 560,972 - -1.4 560,972 - +1.7 560,972 - -2.0 560,973 + +0.4 560,973 + +2.2 561,065 + Pf6N2E2_494 0.19 -1.3 561,134 - Pf6N2E2_494 0.35 +1.7 561,134 - Pf6N2E2_494 0.35 +0.1 561,134 - Pf6N2E2_494 0.35 -1.0 561,180 - Pf6N2E2_494 0.45 +0.7 561,453 - +0.1 561,573 + Pf6N2E2_495 0.11 -1.7 561,637 + Pf6N2E2_495 0.17 -1.6 561,834 + Pf6N2E2_495 0.35 -1.3 562,001 + Pf6N2E2_495 0.50 -2.7 562,085 + Pf6N2E2_495 0.57 -2.4 562,184 + Pf6N2E2_495 0.66 -1.1 562,184 + Pf6N2E2_495 0.66 -2.4 562,607 + +1.1 562,625 + -2.4 562,628 + +3.1 562,640 + +2.7 562,640 + +0.9 562,640 + +1.9 562,640 + -1.3 562,641 - +0.3 562,764 - +2.2 562,867 - -0.6 562,892 - Pf6N2E2_496 0.11 +0.7 562,898 + Pf6N2E2_496 0.12 +1.3 562,898 + Pf6N2E2_496 0.12 +1.7 562,898 + Pf6N2E2_496 0.12 -1.7 562,898 + Pf6N2E2_496 0.12 -0.0 562,898 + Pf6N2E2_496 0.12 -2.5 562,898 + Pf6N2E2_496 0.12 +1.0 562,899 - Pf6N2E2_496 0.12 -0.9 562,902 + Pf6N2E2_496 0.12 -1.0 562,902 + Pf6N2E2_496 0.12 -3.1 562,902 + Pf6N2E2_496 0.12 +1.2 562,903 - -0.9 562,903 - Pf6N2E2_496 0.12 +1.5 562,903 - Pf6N2E2_496 0.12 -0.6 562,903 - Pf6N2E2_496 0.12 -1.9 562,903 - Pf6N2E2_496 0.12 -2.5 562,903 - Pf6N2E2_496 0.12 -0.9 563,021 + Pf6N2E2_496 0.21 -2.2 563,021 + Pf6N2E2_496 0.21 -0.5 563,022 - Pf6N2E2_496 0.22 +3.3 563,022 - Pf6N2E2_496 0.22 +2.3 563,022 - Pf6N2E2_496 0.22 -2.4 563,036 + Pf6N2E2_496 0.23 -2.2 563,036 + Pf6N2E2_496 0.23 -2.3 563,036 + Pf6N2E2_496 0.23 -0.6 563,522 + Pf6N2E2_496 0.61 -1.2 563,522 + Pf6N2E2_496 0.61 -1.9 563,523 - Pf6N2E2_496 0.61 +1.8 563,523 - Pf6N2E2_496 0.61 -0.9 563,523 - Pf6N2E2_496 0.61 -1.9 563,523 - Pf6N2E2_496 0.61 -1.7 563,523 - Pf6N2E2_496 0.61 -1.7 563,523 - Pf6N2E2_496 0.61 -0.9 563,555 + Pf6N2E2_496 0.63 -3.7 563,555 + Pf6N2E2_496 0.63 +1.4 563,556 - Pf6N2E2_496 0.63 -1.6 563,556 - Pf6N2E2_496 0.63 +0.9
Or see this region's nucleotide sequence