Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_491 and Pf6N2E2_492 are separated by 273 nucleotides Pf6N2E2_492 and Pf6N2E2_493 are separated by 55 nucleotides Pf6N2E2_493 and Pf6N2E2_494 are separated by 149 nucleotides Pf6N2E2_494 and Pf6N2E2_495 are separated by 29 nucleotides
Pf6N2E2_491 - hypothetical protein, at 558,482 to 558,835
_491
Pf6N2E2_492 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 559,109 to 559,729
_492
Pf6N2E2_493 - YD repeat protein, at 559,785 to 560,831
_493
Pf6N2E2_494 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 560,981 to 561,418
_494
Pf6N2E2_495 - YD repeat protein, at 561,448 to 562,563
_495
Position (kb) 559
560
561 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 558.796 kb on - strand, within Pf6N2E2_491 at 558.870 kb on - strand at 558.870 kb on - strand at 558.900 kb on - strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 559.018 kb on + strand at 559.018 kb on + strand at 559.019 kb on - strand at 559.141 kb on + strand at 559.142 kb on - strand at 559.256 kb on + strand, within Pf6N2E2_492 at 559.256 kb on + strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.750 kb on + strand at 559.751 kb on - strand at 559.751 kb on - strand at 559.758 kb on - strand at 560.132 kb on + strand, within Pf6N2E2_493 at 560.452 kb on + strand, within Pf6N2E2_493 at 560.452 kb on + strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.729 kb on - strand at 560.729 kb on - strand at 560.729 kb on - strand at 560.894 kb on - strand at 560.896 kb on + strand at 560.908 kb on + strand at 560.909 kb on - strand at 560.909 kb on - strand at 560.927 kb on - strand at 560.946 kb on + strand at 560.946 kb on + strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.949 kb on + strand at 560.949 kb on + strand at 560.949 kb on + strand at 560.950 kb on - strand at 560.950 kb on - strand at 560.971 kb on + strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.973 kb on + strand at 560.973 kb on + strand at 561.065 kb on + strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.180 kb on - strand, within Pf6N2E2_494 at 561.453 kb on - strand at 561.573 kb on + strand, within Pf6N2E2_495 at 561.637 kb on + strand, within Pf6N2E2_495
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 558,796 - Pf6N2E2_491 0.89 +0.8 558,870 - -2.4 558,870 - -0.7 558,900 - -1.7 558,998 + -0.4 558,998 + -1.3 558,998 + -2.6 558,999 - -1.4 558,999 - +0.6 558,999 - -1.9 558,999 - +0.4 558,999 - +0.6 559,018 + +0.8 559,018 + +2.1 559,019 - +1.2 559,141 + +2.3 559,142 - +1.7 559,256 + Pf6N2E2_492 0.24 +2.3 559,256 + Pf6N2E2_492 0.24 -2.0 559,257 - Pf6N2E2_492 0.24 +0.6 559,257 - Pf6N2E2_492 0.24 -0.2 559,259 + Pf6N2E2_492 0.24 -1.1 559,259 + Pf6N2E2_492 0.24 -1.1 559,260 - Pf6N2E2_492 0.24 -1.8 559,260 - Pf6N2E2_492 0.24 +1.9 559,260 - Pf6N2E2_492 0.24 +1.6 559,284 - Pf6N2E2_492 0.28 -2.1 559,284 - Pf6N2E2_492 0.28 +1.3 559,284 - Pf6N2E2_492 0.28 +0.0 559,284 - Pf6N2E2_492 0.28 -2.3 559,284 - Pf6N2E2_492 0.28 -2.3 559,750 + -0.8 559,751 - +2.3 559,751 - +2.2 559,758 - +1.5 560,132 + Pf6N2E2_493 0.33 -1.6 560,452 + Pf6N2E2_493 0.64 -0.3 560,452 + Pf6N2E2_493 0.64 -2.0 560,701 - Pf6N2E2_493 0.87 +1.8 560,701 - Pf6N2E2_493 0.87 +0.3 560,701 - Pf6N2E2_493 0.87 -2.3 560,701 - Pf6N2E2_493 0.87 -1.7 560,729 - +1.6 560,729 - +1.1 560,729 - +0.1 560,894 - +2.0 560,896 + -0.5 560,908 + +0.8 560,909 - -2.2 560,909 - -3.1 560,927 - +1.9 560,946 + +1.1 560,946 + -0.3 560,947 - +0.9 560,947 - -0.4 560,947 - +0.8 560,947 - -0.3 560,947 - -2.1 560,949 + -1.1 560,949 + +2.0 560,949 + +0.5 560,950 - -0.3 560,950 - -2.3 560,971 + -0.1 560,972 - +1.1 560,972 - -1.4 560,972 - +1.7 560,972 - -2.0 560,973 + +0.4 560,973 + +2.2 561,065 + Pf6N2E2_494 0.19 -1.3 561,134 - Pf6N2E2_494 0.35 +1.7 561,134 - Pf6N2E2_494 0.35 +0.1 561,134 - Pf6N2E2_494 0.35 -1.0 561,180 - Pf6N2E2_494 0.45 +0.7 561,453 - +0.1 561,573 + Pf6N2E2_495 0.11 -1.7 561,637 + Pf6N2E2_495 0.17 -1.6
Or see this region's nucleotide sequence