Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_490 and Pf6N2E2_491 are separated by 788 nucleotides Pf6N2E2_491 and Pf6N2E2_492 are separated by 273 nucleotides Pf6N2E2_492 and Pf6N2E2_493 are separated by 55 nucleotides
Pf6N2E2_490 - PhaK-like protein, at 556,392 to 557,693
_490
Pf6N2E2_491 - hypothetical protein, at 558,482 to 558,835
_491
Pf6N2E2_492 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 559,109 to 559,729
_492
Pf6N2E2_493 - YD repeat protein, at 559,785 to 560,831
_493
Position (kb) 558
559 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 557.538 kb on - strand, within Pf6N2E2_490 at 557.694 kb on + strand at 557.694 kb on + strand at 557.759 kb on + strand at 557.803 kb on + strand at 557.803 kb on + strand at 557.949 kb on + strand at 557.968 kb on + strand at 557.968 kb on + strand at 558.118 kb on - strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.155 kb on - strand at 558.155 kb on - strand at 558.175 kb on + strand at 558.175 kb on + strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.197 kb on + strand at 558.197 kb on + strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.231 kb on + strand at 558.232 kb on - strand at 558.245 kb on + strand at 558.246 kb on - strand at 558.246 kb on - strand at 558.306 kb on - strand at 558.397 kb on + strand at 558.397 kb on + strand at 558.409 kb on + strand at 558.410 kb on - strand at 558.423 kb on + strand at 558.424 kb on - strand at 558.424 kb on - strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.481 kb on + strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.578 kb on + strand, within Pf6N2E2_491 at 558.578 kb on + strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.796 kb on - strand, within Pf6N2E2_491 at 558.870 kb on - strand at 558.870 kb on - strand at 558.900 kb on - strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 559.018 kb on + strand at 559.018 kb on + strand at 559.019 kb on - strand at 559.141 kb on + strand at 559.142 kb on - strand at 559.256 kb on + strand, within Pf6N2E2_492 at 559.256 kb on + strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.750 kb on + strand at 559.751 kb on - strand at 559.751 kb on - strand at 559.758 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 557,538 - Pf6N2E2_490 0.88 +1.7 557,694 + -0.0 557,694 + -0.9 557,759 + -1.3 557,803 + -0.1 557,803 + -0.9 557,949 + +1.1 557,968 + -2.6 557,968 + +0.1 558,118 - +0.0 558,154 + -1.7 558,154 + -2.7 558,154 + +0.0 558,154 + -0.4 558,155 - +0.6 558,155 - +0.2 558,175 + -1.6 558,175 + +0.6 558,176 - -2.8 558,176 - +0.2 558,176 - -2.2 558,176 - +0.5 558,197 + +1.5 558,197 + -1.4 558,198 - -0.6 558,198 - -0.8 558,198 - -0.5 558,231 + -1.3 558,232 - -0.1 558,245 + -1.7 558,246 - +0.2 558,246 - +0.2 558,306 - -0.6 558,397 + -0.5 558,397 + +0.9 558,409 + -1.1 558,410 - +2.4 558,423 + -1.4 558,424 - -0.1 558,424 - -1.4 558,458 + -0.8 558,458 + -2.5 558,458 + +0.4 558,459 - -3.3 558,459 - -2.0 558,459 - -0.9 558,461 + +0.9 558,461 + -2.1 558,461 + +1.2 558,462 - +0.3 558,462 - +1.9 558,462 - -0.4 558,462 - -1.7 558,462 - +0.1 558,481 + +0.4 558,482 - +1.6 558,482 - -2.1 558,482 - -0.5 558,482 - -3.4 558,482 - -2.7 558,482 - +2.2 558,482 - +1.7 558,482 - +2.0 558,482 - +0.7 558,482 - -2.2 558,482 - -0.4 558,578 + Pf6N2E2_491 0.27 -0.4 558,578 + Pf6N2E2_491 0.27 -3.1 558,579 - Pf6N2E2_491 0.27 -0.6 558,579 - Pf6N2E2_491 0.27 -0.7 558,579 - Pf6N2E2_491 0.27 -1.3 558,581 + Pf6N2E2_491 0.28 -2.5 558,581 + Pf6N2E2_491 0.28 -1.3 558,582 - Pf6N2E2_491 0.28 +1.5 558,582 - Pf6N2E2_491 0.28 -2.7 558,582 - Pf6N2E2_491 0.28 -0.0 558,796 - Pf6N2E2_491 0.89 +0.8 558,870 - -2.4 558,870 - -0.7 558,900 - -1.7 558,998 + -0.4 558,998 + -1.3 558,998 + -2.6 558,999 - -1.4 558,999 - +0.6 558,999 - -1.9 558,999 - +0.4 558,999 - +0.6 559,018 + +0.8 559,018 + +2.1 559,019 - +1.2 559,141 + +2.3 559,142 - +1.7 559,256 + Pf6N2E2_492 0.24 +2.3 559,256 + Pf6N2E2_492 0.24 -2.0 559,257 - Pf6N2E2_492 0.24 +0.6 559,257 - Pf6N2E2_492 0.24 -0.2 559,259 + Pf6N2E2_492 0.24 -1.1 559,259 + Pf6N2E2_492 0.24 -1.1 559,260 - Pf6N2E2_492 0.24 -1.8 559,260 - Pf6N2E2_492 0.24 +1.9 559,260 - Pf6N2E2_492 0.24 +1.6 559,284 - Pf6N2E2_492 0.28 -2.1 559,284 - Pf6N2E2_492 0.28 +1.3 559,284 - Pf6N2E2_492 0.28 +0.0 559,284 - Pf6N2E2_492 0.28 -2.3 559,284 - Pf6N2E2_492 0.28 -2.3 559,750 + -0.8 559,751 - +2.3 559,751 - +2.2 559,758 - +1.5
Or see this region's nucleotide sequence