Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4715 and Pf6N2E2_4716 are separated by 195 nucleotides Pf6N2E2_4716 and Pf6N2E2_4717 are separated by 308 nucleotides
Pf6N2E2_4715 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 5,451,671 to 5,452,711
_4715
Pf6N2E2_4716 - Membrane protein, putative, at 5,452,907 to 5,453,611
_4716
Pf6N2E2_4717 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,453,920 to 5,455,173
_4717
Position (kb)
5452
5453
5454 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5452.027 kb on - strand, within Pf6N2E2_4715 at 5452.258 kb on - strand, within Pf6N2E2_4715 at 5452.258 kb on - strand, within Pf6N2E2_4715 at 5452.476 kb on + strand, within Pf6N2E2_4715 at 5452.608 kb on + strand at 5452.674 kb on + strand at 5452.674 kb on + strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.903 kb on - strand at 5452.903 kb on - strand at 5452.903 kb on - strand at 5452.983 kb on - strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.145 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.421 kb on - strand, within Pf6N2E2_4716 at 5453.552 kb on + strand at 5453.673 kb on - strand at 5453.784 kb on + strand at 5453.837 kb on + strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.951 kb on - strand at 5454.032 kb on - strand at 5454.082 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.113 kb on - strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.236 kb on - strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.338 kb on - strand, within Pf6N2E2_4717 at 5454.352 kb on + strand, within Pf6N2E2_4717 at 5454.385 kb on + strand, within Pf6N2E2_4717 at 5454.452 kb on - strand, within Pf6N2E2_4717
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 5,452,027 - Pf6N2E2_4715 0.34 -3.0 5,452,258 - Pf6N2E2_4715 0.56 +0.0 5,452,258 - Pf6N2E2_4715 0.56 -0.1 5,452,476 + Pf6N2E2_4715 0.77 -0.3 5,452,608 + +0.0 5,452,674 + +1.3 5,452,674 + -0.9 5,452,729 - +2.1 5,452,729 - +0.1 5,452,729 - -3.2 5,452,729 - -2.7 5,452,902 + -1.7 5,452,902 + -3.0 5,452,902 + -2.1 5,452,902 + +1.4 5,452,903 - -0.7 5,452,903 - +0.3 5,452,903 - +0.1 5,452,983 - Pf6N2E2_4716 0.11 +0.3 5,453,009 + Pf6N2E2_4716 0.14 +1.1 5,453,009 + Pf6N2E2_4716 0.14 +1.3 5,453,009 + Pf6N2E2_4716 0.14 -0.2 5,453,009 + Pf6N2E2_4716 0.14 -1.5 5,453,009 + Pf6N2E2_4716 0.14 +1.3 5,453,010 - Pf6N2E2_4716 0.15 +1.4 5,453,010 - Pf6N2E2_4716 0.15 -2.4 5,453,010 - Pf6N2E2_4716 0.15 -1.1 5,453,010 - Pf6N2E2_4716 0.15 +1.1 5,453,010 - Pf6N2E2_4716 0.15 -2.1 5,453,010 - Pf6N2E2_4716 0.15 +0.6 5,453,010 - Pf6N2E2_4716 0.15 +2.2 5,453,010 - Pf6N2E2_4716 0.15 +2.1 5,453,119 - Pf6N2E2_4716 0.30 -2.6 5,453,119 - Pf6N2E2_4716 0.30 +0.8 5,453,145 - Pf6N2E2_4716 0.34 -1.5 5,453,272 - Pf6N2E2_4716 0.52 +1.4 5,453,272 - Pf6N2E2_4716 0.52 -0.1 5,453,364 - Pf6N2E2_4716 0.65 -0.1 5,453,364 - Pf6N2E2_4716 0.65 -0.0 5,453,420 + Pf6N2E2_4716 0.73 -2.4 5,453,420 + Pf6N2E2_4716 0.73 +1.9 5,453,420 + Pf6N2E2_4716 0.73 -0.8 5,453,421 - Pf6N2E2_4716 0.73 +1.2 5,453,552 + +1.1 5,453,673 - +0.4 5,453,784 + -0.0 5,453,837 + -0.5 5,453,838 - -3.3 5,453,838 - -1.5 5,453,838 - -0.1 5,453,951 - -1.1 5,454,032 - +0.4 5,454,082 + Pf6N2E2_4717 0.13 -2.3 5,454,112 + Pf6N2E2_4717 0.15 -1.6 5,454,112 + Pf6N2E2_4717 0.15 +0.9 5,454,113 - Pf6N2E2_4717 0.15 +1.6 5,454,233 + Pf6N2E2_4717 0.25 +1.0 5,454,233 + Pf6N2E2_4717 0.25 +0.5 5,454,234 - Pf6N2E2_4717 0.25 -0.9 5,454,234 - Pf6N2E2_4717 0.25 -0.7 5,454,236 - Pf6N2E2_4717 0.25 +0.1 5,454,337 + Pf6N2E2_4717 0.33 +0.1 5,454,337 + Pf6N2E2_4717 0.33 -0.6 5,454,338 - Pf6N2E2_4717 0.33 -0.1 5,454,352 + Pf6N2E2_4717 0.34 -2.6 5,454,385 + Pf6N2E2_4717 0.37 +1.9 5,454,452 - Pf6N2E2_4717 0.42 +1.3
Or see this region's nucleotide sequence