Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_462 and Pf6N2E2_463 are separated by 167 nucleotides Pf6N2E2_463 and Pf6N2E2_464 are separated by 4 nucleotides Pf6N2E2_464 and Pf6N2E2_465 are separated by 68 nucleotides Pf6N2E2_465 and Pf6N2E2_466 are separated by 41 nucleotides
Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486
_462
Pf6N2E2_463 - Transcriptional regulatory protein RstA, at 528,654 to 529,355
_463
Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661
_464
Pf6N2E2_465 - Probable transmembrane protein, at 530,730 to 531,371
_465
Pf6N2E2_466 - Nicotinamidase (EC 3.5.1.19), at 531,413 to 532,066
_466
Position (kb) 529
530
531 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 528.391 kb on - strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.548 kb on + strand at 528.711 kb on + strand at 528.712 kb on - strand at 528.948 kb on + strand, within Pf6N2E2_463 at 528.948 kb on + strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.995 kb on + strand, within Pf6N2E2_463 at 529.131 kb on + strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.406 kb on - strand at 529.420 kb on + strand at 529.421 kb on - strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.472 kb on - strand at 529.484 kb on - strand at 529.774 kb on + strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 530.732 kb on - strand at 530.754 kb on - strand at 530.890 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.966 kb on - strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.404 kb on + strand at 531.404 kb on + strand at 531.405 kb on - strand at 531.405 kb on - strand at 531.434 kb on + strand at 531.435 kb on - strand at 531.435 kb on - strand at 531.615 kb on + strand, within Pf6N2E2_466 at 531.615 kb on + strand, within Pf6N2E2_466 at 531.616 kb on - strand, within Pf6N2E2_466
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 528,391 - +2.1 528,546 + +0.9 528,546 + +1.7 528,546 + -2.0 528,546 + -1.6 528,546 + -0.8 528,548 + -2.4 528,711 + +0.5 528,712 - -0.7 528,948 + Pf6N2E2_463 0.42 -1.7 528,948 + Pf6N2E2_463 0.42 +0.4 528,949 - Pf6N2E2_463 0.42 +0.4 528,949 - Pf6N2E2_463 0.42 -1.9 528,949 - Pf6N2E2_463 0.42 -1.3 528,949 - Pf6N2E2_463 0.42 +0.1 528,949 - Pf6N2E2_463 0.42 -1.8 528,995 + Pf6N2E2_463 0.49 +2.6 529,131 + Pf6N2E2_463 0.68 +0.5 529,132 - Pf6N2E2_463 0.68 -2.7 529,132 - Pf6N2E2_463 0.68 -2.5 529,171 + Pf6N2E2_463 0.74 -0.3 529,171 + Pf6N2E2_463 0.74 -0.6 529,172 - Pf6N2E2_463 0.74 +0.5 529,172 - Pf6N2E2_463 0.74 -2.8 529,172 - Pf6N2E2_463 0.74 -1.3 529,406 - +3.2 529,420 + -1.1 529,421 - -0.3 529,471 + -3.0 529,471 + +3.0 529,471 + -1.3 529,471 + -2.7 529,471 + -1.2 529,471 + -1.3 529,472 - -0.1 529,484 - -1.9 529,774 + Pf6N2E2_464 0.32 +0.4 529,775 - Pf6N2E2_464 0.32 +1.5 529,775 - Pf6N2E2_464 0.32 -0.3 530,732 - -1.3 530,754 - +0.2 530,890 + Pf6N2E2_465 0.25 -3.3 530,905 + Pf6N2E2_465 0.27 -0.3 530,905 + Pf6N2E2_465 0.27 -1.8 530,905 + Pf6N2E2_465 0.27 +0.9 530,906 - Pf6N2E2_465 0.27 -1.9 530,906 - Pf6N2E2_465 0.27 -0.9 530,965 + Pf6N2E2_465 0.37 -2.9 530,965 + Pf6N2E2_465 0.37 +0.9 530,965 + Pf6N2E2_465 0.37 -1.1 530,965 + Pf6N2E2_465 0.37 -1.8 530,966 - Pf6N2E2_465 0.37 -1.9 530,968 + Pf6N2E2_465 0.37 -1.2 530,968 + Pf6N2E2_465 0.37 -1.5 530,969 - Pf6N2E2_465 0.37 +2.6 530,969 - Pf6N2E2_465 0.37 -1.5 531,239 - Pf6N2E2_465 0.79 +1.6 531,239 - Pf6N2E2_465 0.79 -0.2 531,239 - Pf6N2E2_465 0.79 +0.4 531,401 + -1.5 531,401 + -2.1 531,401 + +1.3 531,401 + +0.8 531,401 + +1.1 531,404 + +0.7 531,404 + -2.1 531,405 - +1.8 531,405 - +1.5 531,434 + -0.3 531,435 - -0.0 531,435 - +0.5 531,615 + Pf6N2E2_466 0.31 -1.1 531,615 + Pf6N2E2_466 0.31 -2.7 531,616 - Pf6N2E2_466 0.31 -1.9
Or see this region's nucleotide sequence