Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_394 and Pf6N2E2_395 are separated by 337 nucleotides Pf6N2E2_395 and Pf6N2E2_396 are separated by 684 nucleotides
Pf6N2E2_394 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 462,283 to 463,059
_394
Pf6N2E2_395 - Glutathione S-transferase (EC 2.5.1.18), at 463,397 to 463,828
_395
Pf6N2E2_396 - hypothetical protein, at 464,513 to 464,920
_396
Position (kb) 463
464 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 462.648 kb on + strand, within Pf6N2E2_394 at 462.720 kb on + strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.731 kb on - strand, within Pf6N2E2_394 at 462.760 kb on + strand, within Pf6N2E2_394 at 462.761 kb on - strand, within Pf6N2E2_394 at 462.882 kb on + strand, within Pf6N2E2_394 at 462.890 kb on - strand, within Pf6N2E2_394 at 463.008 kb on + strand at 463.009 kb on - strand at 463.009 kb on - strand at 463.082 kb on + strand at 463.082 kb on + strand at 463.082 kb on + strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.170 kb on - strand at 463.184 kb on + strand at 463.271 kb on + strand at 463.271 kb on + strand at 463.272 kb on - strand at 463.272 kb on - strand at 463.435 kb on - strand at 463.558 kb on + strand, within Pf6N2E2_395 at 463.602 kb on + strand, within Pf6N2E2_395 at 463.603 kb on - strand, within Pf6N2E2_395 at 463.697 kb on + strand, within Pf6N2E2_395 at 463.698 kb on - strand, within Pf6N2E2_395 at 463.731 kb on - strand, within Pf6N2E2_395 at 463.795 kb on - strand at 463.795 kb on - strand at 463.882 kb on - strand at 463.924 kb on - strand at 463.957 kb on - strand at 464.009 kb on - strand at 464.009 kb on - strand at 464.475 kb on + strand at 464.476 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.512 kb on - strand at 464.512 kb on - strand at 464.532 kb on + strand at 464.532 kb on + strand at 464.533 kb on - strand at 464.533 kb on - strand at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.690 kb on + strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 462,648 + Pf6N2E2_394 0.47 +1.6 462,720 + Pf6N2E2_394 0.56 +0.7 462,721 - Pf6N2E2_394 0.56 -2.1 462,721 - Pf6N2E2_394 0.56 -3.2 462,721 - Pf6N2E2_394 0.56 +1.3 462,731 - Pf6N2E2_394 0.58 +1.0 462,760 + Pf6N2E2_394 0.61 -0.9 462,761 - Pf6N2E2_394 0.62 -3.3 462,882 + Pf6N2E2_394 0.77 +1.7 462,890 - Pf6N2E2_394 0.78 -1.1 463,008 + -1.1 463,009 - +1.2 463,009 - +0.2 463,082 + -0.9 463,082 + +0.1 463,082 + +1.9 463,083 - +0.6 463,083 - +1.1 463,083 - +2.2 463,083 - -2.9 463,170 - -1.1 463,184 + -0.6 463,271 + -0.1 463,271 + -2.8 463,272 - +1.5 463,272 - -2.0 463,435 - -0.0 463,558 + Pf6N2E2_395 0.37 -1.0 463,602 + Pf6N2E2_395 0.47 -2.1 463,603 - Pf6N2E2_395 0.48 -2.6 463,697 + Pf6N2E2_395 0.69 -2.2 463,698 - Pf6N2E2_395 0.70 -0.1 463,731 - Pf6N2E2_395 0.77 -2.4 463,795 - +3.0 463,795 - -0.9 463,882 - -0.6 463,924 - -1.3 463,957 - -2.2 464,009 - +0.7 464,009 - -0.9 464,475 + -0.6 464,476 - +0.3 464,508 - +2.7 464,508 - +1.8 464,508 - -0.2 464,508 - -1.1 464,511 + +0.6 464,511 + +2.0 464,511 + +3.0 464,511 + -0.3 464,512 - +1.7 464,512 - +1.7 464,532 + +2.0 464,532 + +0.2 464,533 - -0.5 464,533 - -1.3 464,594 - Pf6N2E2_396 0.20 +0.4 464,594 - Pf6N2E2_396 0.20 -0.9 464,594 - Pf6N2E2_396 0.20 +1.4 464,690 + Pf6N2E2_396 0.43 -2.2 464,719 - Pf6N2E2_396 0.50 +1.2 464,719 - Pf6N2E2_396 0.50 +1.2 464,812 + Pf6N2E2_396 0.73 +2.4 464,812 + Pf6N2E2_396 0.73 -0.6 464,812 + Pf6N2E2_396 0.73 +1.6
Or see this region's nucleotide sequence