Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3736 and Pf6N2E2_3737 are separated by 219 nucleotides Pf6N2E2_3737 and Pf6N2E2_3738 overlap by 1 nucleotides Pf6N2E2_3738 and Pf6N2E2_3739 overlap by 4 nucleotides Pf6N2E2_3739 and Pf6N2E2_3740 overlap by 4 nucleotides
Pf6N2E2_3736 - Permeases of the major facilitator superfamily, at 4,352,434 to 4,353,711
_3736
Pf6N2E2_3737 - General secretion pathway protein M, at 4,353,931 to 4,354,377
_3737
Pf6N2E2_3738 - General secretion pathway protein L, at 4,354,377 to 4,355,480
_3738
Pf6N2E2_3739 - General secretion pathway protein K, at 4,355,477 to 4,356,325
_3739
Pf6N2E2_3740 - General secretion pathway protein J, at 4,356,322 to 4,356,855
_3740
Position (kb)
4354
4355
4356 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4353.535 kb on + strand, within Pf6N2E2_3736 at 4353.547 kb on + strand, within Pf6N2E2_3736 at 4353.547 kb on + strand, within Pf6N2E2_3736 at 4353.548 kb on - strand, within Pf6N2E2_3736 at 4353.548 kb on - strand, within Pf6N2E2_3736 at 4353.574 kb on - strand, within Pf6N2E2_3736 at 4353.709 kb on + strand at 4353.751 kb on + strand at 4353.751 kb on + strand at 4353.752 kb on - strand at 4353.966 kb on + strand at 4353.966 kb on + strand at 4353.967 kb on - strand at 4353.967 kb on - strand at 4353.967 kb on - strand at 4353.967 kb on - strand at 4354.223 kb on + strand, within Pf6N2E2_3737 at 4354.277 kb on + strand, within Pf6N2E2_3737 at 4354.358 kb on - strand at 4354.358 kb on - strand at 4354.358 kb on - strand at 4354.358 kb on - strand at 4354.365 kb on + strand at 4354.365 kb on + strand at 4354.366 kb on - strand at 4354.366 kb on - strand at 4354.366 kb on - strand at 4354.366 kb on - strand at 4354.727 kb on - strand, within Pf6N2E2_3738 at 4354.727 kb on - strand, within Pf6N2E2_3738 at 4354.791 kb on + strand, within Pf6N2E2_3738 at 4354.792 kb on - strand, within Pf6N2E2_3738 at 4355.100 kb on + strand, within Pf6N2E2_3738 at 4355.100 kb on + strand, within Pf6N2E2_3738 at 4355.464 kb on + strand at 4355.798 kb on - strand, within Pf6N2E2_3739 at 4355.933 kb on + strand, within Pf6N2E2_3739 at 4356.010 kb on + strand, within Pf6N2E2_3739 at 4356.010 kb on + strand, within Pf6N2E2_3739 at 4356.011 kb on - strand, within Pf6N2E2_3739 at 4356.110 kb on + strand, within Pf6N2E2_3739 at 4356.111 kb on - strand, within Pf6N2E2_3739 at 4356.128 kb on + strand, within Pf6N2E2_3739 at 4356.129 kb on - strand, within Pf6N2E2_3739 at 4356.143 kb on + strand, within Pf6N2E2_3739 at 4356.144 kb on - strand, within Pf6N2E2_3739 at 4356.144 kb on - strand, within Pf6N2E2_3739 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.476 kb on - strand, within Pf6N2E2_3740 at 4356.476 kb on - strand, within Pf6N2E2_3740
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 4,353,535 + Pf6N2E2_3736 0.86 +0.5 4,353,547 + Pf6N2E2_3736 0.87 -1.5 4,353,547 + Pf6N2E2_3736 0.87 +0.9 4,353,548 - Pf6N2E2_3736 0.87 -1.5 4,353,548 - Pf6N2E2_3736 0.87 +1.9 4,353,574 - Pf6N2E2_3736 0.89 +1.5 4,353,709 + +0.4 4,353,751 + +0.2 4,353,751 + -3.6 4,353,752 - -0.7 4,353,966 + +1.4 4,353,966 + -2.3 4,353,967 - -0.5 4,353,967 - +1.2 4,353,967 - -2.7 4,353,967 - -2.6 4,354,223 + Pf6N2E2_3737 0.65 -2.1 4,354,277 + Pf6N2E2_3737 0.77 -1.4 4,354,358 - -0.1 4,354,358 - +0.6 4,354,358 - +2.4 4,354,358 - +1.6 4,354,365 + -2.6 4,354,365 + +1.5 4,354,366 - +1.5 4,354,366 - -2.4 4,354,366 - +0.4 4,354,366 - +0.2 4,354,727 - Pf6N2E2_3738 0.32 -2.2 4,354,727 - Pf6N2E2_3738 0.32 -2.6 4,354,791 + Pf6N2E2_3738 0.38 -2.8 4,354,792 - Pf6N2E2_3738 0.38 -2.1 4,355,100 + Pf6N2E2_3738 0.65 +0.0 4,355,100 + Pf6N2E2_3738 0.65 -0.9 4,355,464 + +1.2 4,355,798 - Pf6N2E2_3739 0.38 +0.1 4,355,933 + Pf6N2E2_3739 0.54 +2.1 4,356,010 + Pf6N2E2_3739 0.63 +0.7 4,356,010 + Pf6N2E2_3739 0.63 -1.0 4,356,011 - Pf6N2E2_3739 0.63 +1.1 4,356,110 + Pf6N2E2_3739 0.75 +0.5 4,356,111 - Pf6N2E2_3739 0.75 -2.5 4,356,128 + Pf6N2E2_3739 0.77 -2.0 4,356,129 - Pf6N2E2_3739 0.77 -0.8 4,356,143 + Pf6N2E2_3739 0.78 +0.0 4,356,144 - Pf6N2E2_3739 0.79 +1.1 4,356,144 - Pf6N2E2_3739 0.79 +0.3 4,356,455 - Pf6N2E2_3740 0.25 -0.4 4,356,455 - Pf6N2E2_3740 0.25 +0.3 4,356,455 - Pf6N2E2_3740 0.25 +0.2 4,356,475 + Pf6N2E2_3740 0.29 -1.5 4,356,475 + Pf6N2E2_3740 0.29 -2.0 4,356,475 + Pf6N2E2_3740 0.29 +0.3 4,356,476 - Pf6N2E2_3740 0.29 -1.5 4,356,476 - Pf6N2E2_3740 0.29 -1.0
Or see this region's nucleotide sequence