Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_329 and Pf6N2E2_330 are separated by 241 nucleotides Pf6N2E2_330 and Pf6N2E2_331 are separated by 265 nucleotides Pf6N2E2_331 and Pf6N2E2_332 are separated by 23 nucleotides
Pf6N2E2_329 - L-lactate permease, at 396,693 to 398,456
_329
Pf6N2E2_330 - Mesenchymal stem cell protein DSCD75, at 398,698 to 399,249
_330
Pf6N2E2_331 - Putative translation initiation inhibitor, yjgF family, at 399,515 to 399,946
_331
Pf6N2E2_332 - putative nucleoside-diphosphate-sugar epimerase, at 399,970 to 400,848
_332
Position (kb) 398
399
400 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 397.729 kb on + strand, within Pf6N2E2_329 at 397.729 kb on + strand, within Pf6N2E2_329 at 397.730 kb on - strand, within Pf6N2E2_329 at 398.248 kb on + strand, within Pf6N2E2_329 at 398.249 kb on - strand, within Pf6N2E2_329 at 398.437 kb on + strand at 398.438 kb on - strand at 398.474 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.517 kb on - strand at 398.517 kb on - strand at 398.517 kb on - strand at 398.518 kb on + strand at 398.519 kb on - strand at 398.623 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.684 kb on - strand at 398.684 kb on - strand at 399.074 kb on + strand, within Pf6N2E2_330 at 399.074 kb on + strand, within Pf6N2E2_330 at 399.075 kb on - strand, within Pf6N2E2_330 at 399.107 kb on + strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.283 kb on + strand at 399.284 kb on - strand at 399.307 kb on - strand at 399.392 kb on + strand at 399.494 kb on - strand at 399.624 kb on + strand, within Pf6N2E2_331 at 399.624 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.938 kb on - strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.075 kb on + strand, within Pf6N2E2_332 at 400.232 kb on + strand, within Pf6N2E2_332
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 397,729 + Pf6N2E2_329 0.59 -1.8 397,729 + Pf6N2E2_329 0.59 -0.6 397,730 - Pf6N2E2_329 0.59 -1.1 398,248 + Pf6N2E2_329 0.88 +0.1 398,249 - Pf6N2E2_329 0.88 -0.6 398,437 + +0.3 398,438 - +1.1 398,474 + -0.9 398,516 + +0.2 398,516 + -0.5 398,516 + -0.6 398,516 + -3.4 398,516 + -0.0 398,517 - -2.5 398,517 - +1.0 398,517 - -2.2 398,518 + -1.3 398,519 - +1.3 398,623 + +0.7 398,683 + -0.3 398,683 + -1.5 398,683 + -1.0 398,683 + -1.2 398,684 - -0.4 398,684 - -2.0 399,074 + Pf6N2E2_330 0.68 +0.4 399,074 + Pf6N2E2_330 0.68 -0.9 399,075 - Pf6N2E2_330 0.68 +2.0 399,107 + Pf6N2E2_330 0.74 +1.3 399,108 - Pf6N2E2_330 0.74 +2.1 399,108 - Pf6N2E2_330 0.74 -1.3 399,283 + +0.2 399,284 - +1.8 399,307 - -1.2 399,392 + -2.6 399,494 - -0.9 399,624 + Pf6N2E2_331 0.25 -2.5 399,624 + Pf6N2E2_331 0.25 +0.7 399,666 + Pf6N2E2_331 0.35 +2.9 399,666 + Pf6N2E2_331 0.35 +0.3 399,666 + Pf6N2E2_331 0.35 +1.5 399,938 - -1.3 400,016 + -1.1 400,016 + -2.4 400,016 + -1.6 400,016 + -1.2 400,016 + -1.8 400,017 - -1.6 400,017 - +0.6 400,017 - +1.2 400,017 - -1.3 400,017 - +2.3 400,075 + Pf6N2E2_332 0.12 -1.9 400,232 + Pf6N2E2_332 0.30 +0.6
Or see this region's nucleotide sequence