Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_322 and Pf6N2E2_323 are separated by 166 nucleotides Pf6N2E2_323 and Pf6N2E2_324 overlap by 4 nucleotides
Pf6N2E2_322 - Transcriptional regulator, LacI family, at 390,422 to 391,420
_322
Pf6N2E2_323 - Nucleoside-diphosphate-sugar epimerases, at 391,587 to 392,762
_323
Pf6N2E2_324 - Inosose isomerase (EC 5.3.99.-), at 392,759 to 393,850
_324
Position (kb) 391
392
393 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 390.626 kb on - strand, within Pf6N2E2_322 at 391.086 kb on + strand, within Pf6N2E2_322 at 391.086 kb on + strand, within Pf6N2E2_322 at 391.207 kb on + strand, within Pf6N2E2_322 at 391.207 kb on + strand, within Pf6N2E2_322 at 391.207 kb on + strand, within Pf6N2E2_322 at 391.208 kb on - strand, within Pf6N2E2_322 at 391.482 kb on + strand at 391.609 kb on + strand at 391.610 kb on - strand at 391.610 kb on - strand at 391.776 kb on + strand, within Pf6N2E2_323 at 391.993 kb on - strand, within Pf6N2E2_323 at 392.006 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.011 kb on - strand, within Pf6N2E2_323 at 392.138 kb on + strand, within Pf6N2E2_323 at 392.246 kb on + strand, within Pf6N2E2_323 at 392.257 kb on + strand, within Pf6N2E2_323 at 392.257 kb on + strand, within Pf6N2E2_323 at 392.257 kb on + strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.404 kb on - strand, within Pf6N2E2_323 at 392.613 kb on - strand, within Pf6N2E2_323 at 392.764 kb on + strand at 392.803 kb on + strand at 392.885 kb on + strand, within Pf6N2E2_324 at 392.886 kb on - strand, within Pf6N2E2_324 at 392.921 kb on + strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.957 kb on + strand, within Pf6N2E2_324 at 392.958 kb on - strand, within Pf6N2E2_324 at 393.215 kb on + strand, within Pf6N2E2_324 at 393.215 kb on + strand, within Pf6N2E2_324 at 393.216 kb on - strand, within Pf6N2E2_324 at 393.332 kb on + strand, within Pf6N2E2_324 at 393.332 kb on + strand, within Pf6N2E2_324 at 393.425 kb on + strand, within Pf6N2E2_324 at 393.425 kb on + strand, within Pf6N2E2_324 at 393.426 kb on - strand, within Pf6N2E2_324 at 393.461 kb on + strand, within Pf6N2E2_324 at 393.461 kb on + strand, within Pf6N2E2_324 at 393.462 kb on - strand, within Pf6N2E2_324 at 393.462 kb on - strand, within Pf6N2E2_324 at 393.462 kb on - strand, within Pf6N2E2_324
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 390,626 - Pf6N2E2_322 0.20 +0.7 391,086 + Pf6N2E2_322 0.66 -0.4 391,086 + Pf6N2E2_322 0.66 -3.5 391,207 + Pf6N2E2_322 0.79 -0.1 391,207 + Pf6N2E2_322 0.79 +0.9 391,207 + Pf6N2E2_322 0.79 -2.8 391,208 - Pf6N2E2_322 0.79 +1.6 391,482 + -2.6 391,609 + +1.0 391,610 - -1.8 391,610 - -2.6 391,776 + Pf6N2E2_323 0.16 -0.8 391,993 - Pf6N2E2_323 0.35 -0.5 392,006 + Pf6N2E2_323 0.36 +0.7 392,010 + Pf6N2E2_323 0.36 -0.2 392,010 + Pf6N2E2_323 0.36 +2.0 392,010 + Pf6N2E2_323 0.36 -1.8 392,010 + Pf6N2E2_323 0.36 +1.9 392,011 - Pf6N2E2_323 0.36 +1.7 392,138 + Pf6N2E2_323 0.47 +0.5 392,246 + Pf6N2E2_323 0.56 +1.0 392,257 + Pf6N2E2_323 0.57 +2.5 392,257 + Pf6N2E2_323 0.57 +0.5 392,257 + Pf6N2E2_323 0.57 -0.1 392,258 - Pf6N2E2_323 0.57 +0.1 392,258 - Pf6N2E2_323 0.57 +0.2 392,258 - Pf6N2E2_323 0.57 -1.5 392,258 - Pf6N2E2_323 0.57 -1.2 392,404 - Pf6N2E2_323 0.69 -0.0 392,613 - Pf6N2E2_323 0.87 -2.4 392,764 + -0.4 392,803 + +1.2 392,885 + Pf6N2E2_324 0.12 +0.9 392,886 - Pf6N2E2_324 0.12 +0.4 392,921 + Pf6N2E2_324 0.15 -2.5 392,922 - Pf6N2E2_324 0.15 -0.2 392,922 - Pf6N2E2_324 0.15 +1.0 392,922 - Pf6N2E2_324 0.15 -1.6 392,922 - Pf6N2E2_324 0.15 -0.6 392,922 - Pf6N2E2_324 0.15 -2.1 392,957 + Pf6N2E2_324 0.18 +0.5 392,958 - Pf6N2E2_324 0.18 -0.6 393,215 + Pf6N2E2_324 0.42 -1.0 393,215 + Pf6N2E2_324 0.42 +0.9 393,216 - Pf6N2E2_324 0.42 +0.9 393,332 + Pf6N2E2_324 0.52 -1.9 393,332 + Pf6N2E2_324 0.52 +0.5 393,425 + Pf6N2E2_324 0.61 +1.3 393,425 + Pf6N2E2_324 0.61 +1.5 393,426 - Pf6N2E2_324 0.61 -2.8 393,461 + Pf6N2E2_324 0.64 +0.1 393,461 + Pf6N2E2_324 0.64 -1.3 393,462 - Pf6N2E2_324 0.64 +0.0 393,462 - Pf6N2E2_324 0.64 +0.1 393,462 - Pf6N2E2_324 0.64 -2.5
Or see this region's nucleotide sequence