Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3217 and Pf6N2E2_3218 are separated by 96 nucleotides Pf6N2E2_3218 and Pf6N2E2_3219 are separated by 42 nucleotides Pf6N2E2_3219 and Pf6N2E2_3220 overlap by 6 nucleotides
Pf6N2E2_3217 - Hemolysin activation/secretion protein, at 3,715,792 to 3,717,456
_3217
Pf6N2E2_3218 - Large exoproteins involved in heme utilization or adhesion, at 3,717,553 to 3,725,181
_3218
Pf6N2E2_3219 - hypothetical protein, at 3,725,224 to 3,725,718
_3219
Pf6N2E2_3220 - hypothetical protein, at 3,725,713 to 3,727,221
_3220
Position (kb)
3717
3718
3719
3720
3721
3722
3723
3724
3725
3726 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3716.592 kb on - strand, within Pf6N2E2_3217 at 3716.654 kb on + strand, within Pf6N2E2_3217 at 3716.654 kb on + strand, within Pf6N2E2_3217 at 3716.654 kb on + strand, within Pf6N2E2_3217 at 3716.655 kb on - strand, within Pf6N2E2_3217 at 3716.655 kb on - strand, within Pf6N2E2_3217 at 3716.732 kb on - strand, within Pf6N2E2_3217 at 3716.732 kb on - strand, within Pf6N2E2_3217 at 3716.732 kb on - strand, within Pf6N2E2_3217 at 3716.732 kb on - strand, within Pf6N2E2_3217 at 3716.902 kb on + strand, within Pf6N2E2_3217 at 3716.902 kb on + strand, within Pf6N2E2_3217 at 3716.902 kb on + strand, within Pf6N2E2_3217 at 3716.902 kb on + strand, within Pf6N2E2_3217 at 3716.903 kb on - strand, within Pf6N2E2_3217 at 3716.903 kb on - strand, within Pf6N2E2_3217 at 3717.078 kb on - strand, within Pf6N2E2_3217 at 3717.110 kb on + strand, within Pf6N2E2_3217 at 3717.292 kb on + strand at 3717.292 kb on + strand at 3717.292 kb on + strand at 3717.292 kb on + strand at 3717.292 kb on + strand at 3717.292 kb on + strand at 3717.293 kb on - strand at 3717.293 kb on - strand at 3717.295 kb on - strand at 3717.450 kb on + strand at 3717.450 kb on + strand at 3717.451 kb on - strand at 3718.061 kb on + strand at 3718.062 kb on - strand at 3718.118 kb on + strand at 3720.969 kb on + strand, within Pf6N2E2_3218 at 3721.148 kb on + strand, within Pf6N2E2_3218 at 3721.149 kb on - strand, within Pf6N2E2_3218 at 3721.310 kb on - strand, within Pf6N2E2_3218 at 3721.795 kb on - strand, within Pf6N2E2_3218 at 3722.056 kb on + strand, within Pf6N2E2_3218 at 3722.644 kb on - strand, within Pf6N2E2_3218 at 3722.644 kb on - strand, within Pf6N2E2_3218 at 3722.703 kb on - strand, within Pf6N2E2_3218 at 3722.882 kb on + strand, within Pf6N2E2_3218 at 3722.897 kb on + strand, within Pf6N2E2_3218 at 3722.897 kb on + strand, within Pf6N2E2_3218 at 3722.897 kb on + strand, within Pf6N2E2_3218 at 3722.897 kb on + strand, within Pf6N2E2_3218 at 3722.897 kb on + strand, within Pf6N2E2_3218 at 3722.898 kb on - strand, within Pf6N2E2_3218 at 3722.898 kb on - strand, within Pf6N2E2_3218 at 3722.898 kb on - strand, within Pf6N2E2_3218 at 3722.898 kb on - strand, within Pf6N2E2_3218 at 3722.898 kb on - strand, within Pf6N2E2_3218 at 3722.901 kb on + strand, within Pf6N2E2_3218 at 3723.030 kb on + strand, within Pf6N2E2_3218 at 3723.030 kb on + strand, within Pf6N2E2_3218 at 3723.107 kb on + strand, within Pf6N2E2_3218 at 3723.108 kb on - strand, within Pf6N2E2_3218 at 3723.108 kb on - strand, within Pf6N2E2_3218 at 3723.108 kb on - strand, within Pf6N2E2_3218 at 3723.108 kb on - strand, within Pf6N2E2_3218 at 3723.321 kb on - strand, within Pf6N2E2_3218 at 3723.321 kb on - strand, within Pf6N2E2_3218 at 3723.360 kb on + strand, within Pf6N2E2_3218 at 3723.392 kb on + strand, within Pf6N2E2_3218 at 3723.393 kb on - strand, within Pf6N2E2_3218 at 3723.393 kb on - strand, within Pf6N2E2_3218 at 3723.393 kb on - strand, within Pf6N2E2_3218 at 3723.393 kb on - strand, within Pf6N2E2_3218 at 3723.393 kb on - strand, within Pf6N2E2_3218 at 3723.514 kb on + strand, within Pf6N2E2_3218 at 3723.515 kb on - strand, within Pf6N2E2_3218 at 3723.515 kb on - strand, within Pf6N2E2_3218 at 3723.639 kb on + strand, within Pf6N2E2_3218 at 3723.639 kb on + strand, within Pf6N2E2_3218 at 3723.639 kb on + strand, within Pf6N2E2_3218 at 3723.640 kb on - strand, within Pf6N2E2_3218 at 3723.640 kb on - strand, within Pf6N2E2_3218 at 3723.653 kb on + strand, within Pf6N2E2_3218 at 3723.654 kb on - strand, within Pf6N2E2_3218 at 3723.654 kb on - strand, within Pf6N2E2_3218 at 3723.654 kb on - strand, within Pf6N2E2_3218 at 3723.654 kb on - strand, within Pf6N2E2_3218 at 3723.728 kb on + strand, within Pf6N2E2_3218 at 3723.729 kb on - strand, within Pf6N2E2_3218 at 3723.750 kb on + strand, within Pf6N2E2_3218 at 3723.751 kb on - strand, within Pf6N2E2_3218 at 3723.751 kb on - strand, within Pf6N2E2_3218 at 3724.187 kb on + strand, within Pf6N2E2_3218 at 3724.187 kb on + strand, within Pf6N2E2_3218 at 3724.187 kb on + strand, within Pf6N2E2_3218 at 3724.187 kb on + strand, within Pf6N2E2_3218 at 3724.187 kb on + strand, within Pf6N2E2_3218 at 3724.187 kb on + strand, within Pf6N2E2_3218 at 3724.188 kb on - strand, within Pf6N2E2_3218 at 3724.188 kb on - strand, within Pf6N2E2_3218 at 3724.188 kb on - strand, within Pf6N2E2_3218 at 3724.188 kb on - strand, within Pf6N2E2_3218 at 3724.188 kb on - strand, within Pf6N2E2_3218 at 3724.581 kb on + strand at 3724.710 kb on - strand at 3724.710 kb on - strand at 3724.768 kb on + strand at 3724.861 kb on + strand at 3724.861 kb on + strand at 3724.862 kb on - strand at 3724.971 kb on + strand at 3725.096 kb on - strand at 3725.122 kb on - strand at 3725.164 kb on + strand at 3725.165 kb on - strand at 3725.165 kb on - strand at 3725.235 kb on + strand at 3725.235 kb on + strand at 3725.238 kb on + strand at 3725.238 kb on + strand at 3725.238 kb on + strand at 3725.238 kb on + strand at 3725.476 kb on + strand, within Pf6N2E2_3219 at 3725.476 kb on + strand, within Pf6N2E2_3219 at 3725.477 kb on - strand, within Pf6N2E2_3219 at 3725.477 kb on - strand, within Pf6N2E2_3219 at 3725.477 kb on - strand, within Pf6N2E2_3219 at 3725.673 kb on + strand at 3725.791 kb on + strand at 3725.791 kb on + strand at 3725.792 kb on - strand at 3725.795 kb on - strand at 3725.795 kb on - strand at 3725.795 kb on - strand at 3725.911 kb on + strand, within Pf6N2E2_3220 at 3726.127 kb on + strand, within Pf6N2E2_3220 at 3726.128 kb on - strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.154 kb on + strand, within Pf6N2E2_3220 at 3726.154 kb on + strand, within Pf6N2E2_3220 at 3726.154 kb on + strand, within Pf6N2E2_3220 at 3726.155 kb on - strand, within Pf6N2E2_3220 at 3726.155 kb on - strand, within Pf6N2E2_3220 at 3726.160 kb on + strand, within Pf6N2E2_3220
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 3,716,592 - Pf6N2E2_3217 0.48 +2.1 3,716,654 + Pf6N2E2_3217 0.52 -2.7 3,716,654 + Pf6N2E2_3217 0.52 -2.3 3,716,654 + Pf6N2E2_3217 0.52 -1.8 3,716,655 - Pf6N2E2_3217 0.52 +0.1 3,716,655 - Pf6N2E2_3217 0.52 -1.6 3,716,732 - Pf6N2E2_3217 0.56 +0.9 3,716,732 - Pf6N2E2_3217 0.56 +0.7 3,716,732 - Pf6N2E2_3217 0.56 +0.0 3,716,732 - Pf6N2E2_3217 0.56 +1.6 3,716,902 + Pf6N2E2_3217 0.67 -0.7 3,716,902 + Pf6N2E2_3217 0.67 -0.6 3,716,902 + Pf6N2E2_3217 0.67 +1.4 3,716,902 + Pf6N2E2_3217 0.67 +0.6 3,716,903 - Pf6N2E2_3217 0.67 -0.3 3,716,903 - Pf6N2E2_3217 0.67 +1.2 3,717,078 - Pf6N2E2_3217 0.77 -0.5 3,717,110 + Pf6N2E2_3217 0.79 -1.1 3,717,292 + +0.8 3,717,292 + -2.1 3,717,292 + -2.3 3,717,292 + -1.8 3,717,292 + +0.0 3,717,292 + +1.1 3,717,293 - -2.3 3,717,293 - +0.0 3,717,295 - -1.5 3,717,450 + +0.1 3,717,450 + +0.9 3,717,451 - -1.4 3,718,061 + -0.6 3,718,062 - -0.2 3,718,118 + -2.1 3,720,969 + Pf6N2E2_3218 0.45 -1.4 3,721,148 + Pf6N2E2_3218 0.47 +1.2 3,721,149 - Pf6N2E2_3218 0.47 +0.2 3,721,310 - Pf6N2E2_3218 0.49 +0.1 3,721,795 - Pf6N2E2_3218 0.56 +1.7 3,722,056 + Pf6N2E2_3218 0.59 +1.6 3,722,644 - Pf6N2E2_3218 0.67 +0.2 3,722,644 - Pf6N2E2_3218 0.67 -1.9 3,722,703 - Pf6N2E2_3218 0.68 +1.1 3,722,882 + Pf6N2E2_3218 0.70 -0.7 3,722,897 + Pf6N2E2_3218 0.70 +1.0 3,722,897 + Pf6N2E2_3218 0.70 +2.6 3,722,897 + Pf6N2E2_3218 0.70 -2.8 3,722,897 + Pf6N2E2_3218 0.70 -0.2 3,722,897 + Pf6N2E2_3218 0.70 +1.6 3,722,898 - Pf6N2E2_3218 0.70 -0.3 3,722,898 - Pf6N2E2_3218 0.70 +2.1 3,722,898 - Pf6N2E2_3218 0.70 +1.6 3,722,898 - Pf6N2E2_3218 0.70 +0.3 3,722,898 - Pf6N2E2_3218 0.70 +0.5 3,722,901 + Pf6N2E2_3218 0.70 +1.8 3,723,030 + Pf6N2E2_3218 0.72 -2.0 3,723,030 + Pf6N2E2_3218 0.72 +2.9 3,723,107 + Pf6N2E2_3218 0.73 +1.0 3,723,108 - Pf6N2E2_3218 0.73 +2.7 3,723,108 - Pf6N2E2_3218 0.73 -0.1 3,723,108 - Pf6N2E2_3218 0.73 -2.7 3,723,108 - Pf6N2E2_3218 0.73 +1.5 3,723,321 - Pf6N2E2_3218 0.76 -0.6 3,723,321 - Pf6N2E2_3218 0.76 +0.1 3,723,360 + Pf6N2E2_3218 0.76 +2.2 3,723,392 + Pf6N2E2_3218 0.77 +1.5 3,723,393 - Pf6N2E2_3218 0.77 -0.4 3,723,393 - Pf6N2E2_3218 0.77 +1.4 3,723,393 - Pf6N2E2_3218 0.77 -0.3 3,723,393 - Pf6N2E2_3218 0.77 +1.3 3,723,393 - Pf6N2E2_3218 0.77 +1.0 3,723,514 + Pf6N2E2_3218 0.78 -1.4 3,723,515 - Pf6N2E2_3218 0.78 +0.1 3,723,515 - Pf6N2E2_3218 0.78 -0.4 3,723,639 + Pf6N2E2_3218 0.80 -0.3 3,723,639 + Pf6N2E2_3218 0.80 -1.7 3,723,639 + Pf6N2E2_3218 0.80 +0.6 3,723,640 - Pf6N2E2_3218 0.80 -1.1 3,723,640 - Pf6N2E2_3218 0.80 -2.6 3,723,653 + Pf6N2E2_3218 0.80 -0.7 3,723,654 - Pf6N2E2_3218 0.80 +1.0 3,723,654 - Pf6N2E2_3218 0.80 -1.6 3,723,654 - Pf6N2E2_3218 0.80 +1.6 3,723,654 - Pf6N2E2_3218 0.80 +0.5 3,723,728 + Pf6N2E2_3218 0.81 -2.5 3,723,729 - Pf6N2E2_3218 0.81 +1.2 3,723,750 + Pf6N2E2_3218 0.81 +1.3 3,723,751 - Pf6N2E2_3218 0.81 -0.9 3,723,751 - Pf6N2E2_3218 0.81 +1.7 3,724,187 + Pf6N2E2_3218 0.87 -1.7 3,724,187 + Pf6N2E2_3218 0.87 +0.4 3,724,187 + Pf6N2E2_3218 0.87 -2.7 3,724,187 + Pf6N2E2_3218 0.87 +1.7 3,724,187 + Pf6N2E2_3218 0.87 -2.6 3,724,187 + Pf6N2E2_3218 0.87 +2.0 3,724,188 - Pf6N2E2_3218 0.87 +1.6 3,724,188 - Pf6N2E2_3218 0.87 +0.6 3,724,188 - Pf6N2E2_3218 0.87 -3.1 3,724,188 - Pf6N2E2_3218 0.87 -0.7 3,724,188 - Pf6N2E2_3218 0.87 +0.4 3,724,581 + -2.7 3,724,710 - -0.1 3,724,710 - +0.1 3,724,768 + +2.2 3,724,861 + +0.7 3,724,861 + +0.1 3,724,862 - +0.3 3,724,971 + -1.3 3,725,096 - +0.8 3,725,122 - +0.6 3,725,164 + +0.3 3,725,165 - -1.8 3,725,165 - -2.3 3,725,235 + -1.1 3,725,235 + -0.9 3,725,238 + -0.7 3,725,238 + +0.7 3,725,238 + -0.2 3,725,238 + -0.3 3,725,476 + Pf6N2E2_3219 0.51 -0.7 3,725,476 + Pf6N2E2_3219 0.51 +2.3 3,725,477 - Pf6N2E2_3219 0.51 -1.8 3,725,477 - Pf6N2E2_3219 0.51 -0.5 3,725,477 - Pf6N2E2_3219 0.51 +2.2 3,725,673 + -0.1 3,725,791 + +1.1 3,725,791 + -2.2 3,725,792 - +0.5 3,725,795 - +1.1 3,725,795 - -0.1 3,725,795 - -1.0 3,725,911 + Pf6N2E2_3220 0.13 -0.5 3,726,127 + Pf6N2E2_3220 0.27 +2.0 3,726,128 - Pf6N2E2_3220 0.28 -1.1 3,726,142 + Pf6N2E2_3220 0.28 +0.8 3,726,142 + Pf6N2E2_3220 0.28 +1.6 3,726,142 + Pf6N2E2_3220 0.28 +0.4 3,726,142 + Pf6N2E2_3220 0.28 +1.0 3,726,142 + Pf6N2E2_3220 0.28 -2.1 3,726,143 - Pf6N2E2_3220 0.28 +0.7 3,726,143 - Pf6N2E2_3220 0.28 -2.8 3,726,143 - Pf6N2E2_3220 0.28 -2.3 3,726,143 - Pf6N2E2_3220 0.28 +1.0 3,726,143 - Pf6N2E2_3220 0.28 -0.7 3,726,143 - Pf6N2E2_3220 0.28 +0.5 3,726,143 - Pf6N2E2_3220 0.28 +0.4 3,726,154 + Pf6N2E2_3220 0.29 -0.7 3,726,154 + Pf6N2E2_3220 0.29 +0.4 3,726,154 + Pf6N2E2_3220 0.29 +0.8 3,726,155 - Pf6N2E2_3220 0.29 -0.2 3,726,155 - Pf6N2E2_3220 0.29 -0.7 3,726,160 + Pf6N2E2_3220 0.30 -0.7
Or see this region's nucleotide sequence