Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_318 and Pf6N2E2_319 are separated by 206 nucleotides Pf6N2E2_319 and Pf6N2E2_320 overlap by 4 nucleotides
Pf6N2E2_318 - Gluconolactonase (EC 3.1.1.17), at 385,447 to 386,538
_318
Pf6N2E2_319 - DUF1850 domain-containing protein, at 386,745 to 387,131
_319
Pf6N2E2_320 - TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1, at 387,128 to 389,155
_320
Position (kb) 386
387
388 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 385.911 kb on + strand, within Pf6N2E2_318 at 386.030 kb on - strand, within Pf6N2E2_318 at 386.071 kb on + strand, within Pf6N2E2_318 at 386.167 kb on + strand, within Pf6N2E2_318 at 386.167 kb on + strand, within Pf6N2E2_318 at 386.168 kb on - strand, within Pf6N2E2_318 at 386.497 kb on + strand at 386.497 kb on + strand at 386.497 kb on + strand at 386.498 kb on - strand at 386.498 kb on - strand at 386.498 kb on - strand at 386.605 kb on - strand at 386.614 kb on - strand at 386.841 kb on + strand, within Pf6N2E2_319 at 386.842 kb on - strand, within Pf6N2E2_319 at 386.842 kb on - strand, within Pf6N2E2_319 at 386.912 kb on - strand, within Pf6N2E2_319 at 387.351 kb on + strand, within Pf6N2E2_320 at 387.352 kb on - strand, within Pf6N2E2_320 at 387.400 kb on - strand, within Pf6N2E2_320 at 387.400 kb on - strand, within Pf6N2E2_320 at 387.400 kb on - strand, within Pf6N2E2_320 at 387.546 kb on + strand, within Pf6N2E2_320 at 387.546 kb on + strand, within Pf6N2E2_320 at 387.627 kb on + strand, within Pf6N2E2_320 at 387.627 kb on + strand, within Pf6N2E2_320 at 387.627 kb on + strand, within Pf6N2E2_320 at 387.628 kb on - strand, within Pf6N2E2_320 at 387.646 kb on + strand, within Pf6N2E2_320 at 387.726 kb on + strand, within Pf6N2E2_320 at 387.830 kb on + strand, within Pf6N2E2_320 at 387.830 kb on + strand, within Pf6N2E2_320 at 387.831 kb on - strand, within Pf6N2E2_320 at 387.993 kb on + strand, within Pf6N2E2_320 at 387.994 kb on - strand, within Pf6N2E2_320 at 388.099 kb on - strand, within Pf6N2E2_320 at 388.099 kb on - strand, within Pf6N2E2_320
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 385,911 + Pf6N2E2_318 0.42 -1.5 386,030 - Pf6N2E2_318 0.53 -2.5 386,071 + Pf6N2E2_318 0.57 +1.4 386,167 + Pf6N2E2_318 0.66 +2.1 386,167 + Pf6N2E2_318 0.66 +1.3 386,168 - Pf6N2E2_318 0.66 +0.9 386,497 + +1.5 386,497 + -3.1 386,497 + -1.8 386,498 - +0.7 386,498 - -1.1 386,498 - -1.4 386,605 - -0.9 386,614 - +1.2 386,841 + Pf6N2E2_319 0.25 +0.5 386,842 - Pf6N2E2_319 0.25 +2.0 386,842 - Pf6N2E2_319 0.25 -0.4 386,912 - Pf6N2E2_319 0.43 +0.8 387,351 + Pf6N2E2_320 0.11 +2.5 387,352 - Pf6N2E2_320 0.11 -0.2 387,400 - Pf6N2E2_320 0.13 +0.6 387,400 - Pf6N2E2_320 0.13 -1.5 387,400 - Pf6N2E2_320 0.13 -0.2 387,546 + Pf6N2E2_320 0.21 +2.1 387,546 + Pf6N2E2_320 0.21 +1.1 387,627 + Pf6N2E2_320 0.25 +1.5 387,627 + Pf6N2E2_320 0.25 +1.9 387,627 + Pf6N2E2_320 0.25 -0.3 387,628 - Pf6N2E2_320 0.25 -1.3 387,646 + Pf6N2E2_320 0.26 +1.8 387,726 + Pf6N2E2_320 0.29 -0.3 387,830 + Pf6N2E2_320 0.35 +0.2 387,830 + Pf6N2E2_320 0.35 +0.1 387,831 - Pf6N2E2_320 0.35 -1.3 387,993 + Pf6N2E2_320 0.43 -0.9 387,994 - Pf6N2E2_320 0.43 -0.8 388,099 - Pf6N2E2_320 0.48 +1.2 388,099 - Pf6N2E2_320 0.48 -2.7
Or see this region's nucleotide sequence